scholarly journals Notas sobre la biología y ecología de Anastrepha obliqua (Diptera: Tephritidae), plaga de plantas anarcardiaceas en América tropical. I. Formas inmaduras.

2016 ◽  
Vol 8 (2) ◽  
pp. 116 ◽  
Author(s):  
Julia Soto-Manitiu ◽  
Luis G. Chaverri ◽  
Luis Fernando Jirón
Keyword(s):  
Small Groups ◽  
Mass Rearing ◽  
Biological Cycle ◽  
Larval Population ◽  
Minute Period ◽  
Anastrepha Obliqua ◽  
Oviposition Substrate ◽  
Fallen Fruit

Some details on the biology, behavior and laboratory mass rearing of Anastrepha obliqua are offered. Information on larvaI diets and oviposition substrates are discussed. Eggs of A. obliqua are, very succeptible to dehydratation and they collapse just few minutes after oviposition, if substrate for oviposition is not near 100% R.H. When using fruits as oviposition substrate, smallerfruit species, Spanish plums (Spondias spp.) offer higher yields. Bigger fruits (mango) loose large amounts of water and it accumulates in the sand substrate drowning mature larvae. After analyzing the biological cycle, the weaker part seems to be the 20 minute period in which mature larvae abandon the fallen fruit substrate and crawl few a centimeters on the floor seeking for an appropiate place for ovipositing. In this period large amounts of predators easily diminish larval population. Once pupation takes place, adult forms hatch in small groups, after eleven days, when atmosferic relativehumidity reaches 70%. Another observations are also included.

New larval agar-based diet for laboratory rearing of Mediterranean fruit fly Ceratitis capitata (Diptera, Tephritidae)

Biologia ◽  
2007 ◽  
Vol 62 (4) ◽  
Author(s):  
Martina Pašková
Keyword(s):  
Ceratitis Capitata ◽  
Potato Starch ◽  
Fruit Fly ◽  
Mass Rearing ◽  
Pupal Weight ◽  
Larval Population ◽  
Laboratory Rearing ◽  
Heat Treated ◽  
New Formulation ◽  
The Cost

AbstractFive larval diets for laboratory rearing of Ceratitis capitata were tested. These diets were based on wheat bran, microcellulose, potato starch and agar. To evaluate the quality of diet, pupal rearing efficacy and pupal weight were checked. The best results were obtained with an agar based diet used for Manduca sexta laboratory rearing. To simplify the preparation and to reduce the cost of this diet, a new formulation was developed. Larvae reared on the new agar-based diet achieved higher pupal rearing efficacy than larvae reared on bran diet recently used in medfly mass rearing facilities. Heat treated medflies reared on the new agar-based diet achieved similar pupal rearing efficacy with heat treated medflies reared on bran diet. When testing population density, higher pupal rearing efficacy was again achieved on new diet. The highest pupal rearing efficacy was achieved with 100 eggs per 25 g of diet, lowest with 500 eggs per 25 g of diet. Concerning pupal weight, there was no difference in results achieved on Petri dishes with different larval population densities. Larvae reared on new agar-based diet reached better results than larvae reared on bran diet. The preparation of the new diet is simple and the cost is low, so it is good for laboratory tests and rearing.

scholarly journals Evaluation of a pelleted diet for larval mass‐rearing of Anastrepha ludens and Anastrepha obliqua

2020 ◽  
Vol 168 (6-7) ◽  
pp. 502-512 ◽  
Author(s):  
Marysol Aceituno‐Medina ◽  
José Pedro Rivera‐Ciprian ◽  
Emilio Hernández
Keyword(s):  
Mass Rearing ◽  
Anastrepha Ludens ◽  
Anastrepha Obliqua ◽  
Pelleted Diet

scholarly journals Facilitated harvesting of eggs from laboratory-reared Chrysoperla externa (Neuroptera: Chrysopidae)

2016 ◽  
Vol 42 (2) ◽  
pp. 133 ◽  
Author(s):  
Ana Luiza Viana Sousa ◽  
Brígida Souza ◽  
Carlos Eduardo Souza Bezerra ◽  
Bruno Barbosa Amaral
Keyword(s):  
Complete Removal ◽  
Mass Rearing ◽  
Growth Chamber ◽  
Embryonic Stage ◽  
Control Programs ◽  
Microtiter Plates ◽  
Oviposition Substrate ◽  
Stage Development ◽  
Chemical Techniques ◽  
Egg Destruction

Chrysoperla externa (Neuroptera: Chrysopidae) eggs are attached to the oviposition substrate by long silk stalks. The complete removal of these stalks is crucial for efficient egg release in biological control programs. The present study aimed at establishing an appropriate oviposition substrate and determining the best embryonic stage for submission of C. externa eggs to manual destalking and harvesting. Eggs oviposited on bond or chamois paper substrates were transferred from rearing cages and incubated in a growth chamber under controlled conditions for 24, 48, 72 or 96 hours according to the embryonic stage development required. Substrates were positioned in an inclined tray and softly brushed with a folded rectangle of soft muslin cloth. Destalked eggs were placed individually in microtiter plates and incubated in a growth chamber until hatching. Egg destruction at all embryonic stages and oviposition on the chamois substrate were considerably higher as compared to those from bond paper. Young eggs harvested from chamois paper were particularly susceptible and exhibited 88 % destruction, whereas eggs aged 48, 72 or 96 hours showed < 10 % destruction on both substrates. Viability of eggs collected at 24 hours for both substrates was significantly different from the observed for the other embryonic stages. The method described will contribute to improve the efficiency of manual harvesting of C. externa eggs and can be employed as an alternative to chemical techniques of destalking in mass rearing.

Anastrepha obliqua (Diptera: Tephritidae) mass-rearing: effect of relaxed colony management

2014 ◽  
Vol 34 (S1) ◽  
pp. S19-S27 ◽  
Author(s):  
Dina Orozco-Dávila ◽  
Trinidad Artiaga-López ◽  
Ma. Del Refugio Hernández ◽  
Julio Domínguez ◽  
Emilio Hernández
Keyword(s):  
Mass Rearing ◽  
Colony Management ◽  
Anastrepha Obliqua

scholarly journals The fruit fly Anastrepha obliqua harbors three kingdoms of life in its intestinal tract.

2021 ◽  
Author(s):  
Gerardo Ruiz Amores ◽  
Gloria Zepeda-Ramos ◽  
Luz Veronica Garcia-Fajardo ◽  
Emilio Hernandez ◽  
Karina Guillen-Navarro
Keyword(s):  
Denaturing Gradient Gel Electrophoresis ◽  
Fruit Fly ◽  
Mass Rearing ◽  
16S Sequencing ◽  
Microbial Species ◽  
Anastrepha Obliqua ◽  
Important Pest ◽  
Important Player ◽  
In The Wild ◽  
Gradient Gel Electrophoresis

The fruit fly Anastrepha obliqua is an economically important pest for mango fruits in Mexico. The sterile insect technique is used to control this pest; it involves mass production and release of sterile flies to reduce the reproduction of the wild population. As noted in different tephritidae, the performance of sterile males may be affected by the assimilation of nutrients under mass-rearing conditions. In the wild, the fly's life cycle suggests the acquisition of different organisms that could modulate the fitness and physiology of the fly. Therefore, the microorganisms lodged in the gut may be determinative. For A. obliqua, there is no information regarding microorganisms other than bacteria. This study analyzed bacteria, fungi, and archaea communities in the A. obliqua gut through denaturing gradient gel electrophoresis (DGGE) profiles of 16S and 18S ribosomal DNA markers. Besides, 16S sequencing and phylogenetic analysis provided a better description of bacteria and archaea communities. We found that wild flies presented higher microbial diversity than laboratory samples. Phylogeny analyses of wild samples suggest the presence of microbial species related to fructose assimilation while laboratory microbial species suggest the presence of microorganisms leading to a specialized metabolism to process yeast as a result of the consumption of an artificial diet. Here, the archaea kingdom is suggested as an important player in fly metabolism. This is the first report of the intestinal microbial (bacteria, archaea, and fungi) composition of A. obliqua, which will aid in our understanding of the role of microorganisms in the development and physiology of the flies.

scholarly journals Notas sobre la biología y ecología de Anastrepha obliqua (diptera: tephritidae), plaga de plantas anacardiáceas en América tropical. II. formas adultas.

2006 ◽  
Vol 10 (2) ◽  
pp. 99 ◽  
Author(s):  
Luis G. Chaverri ◽  
Julia Soto-Manitiu ◽  
Luis Fernando Jirón
Keyword(s):  
Adult Emergence ◽  
Mass Rearing ◽  
Adult Survival ◽  
Mortality Factor ◽  
Laboratory Conditions ◽  
Anastrepha Obliqua ◽  
Similar Survival ◽  
Gravid Females ◽  
High Degree ◽  
Hymenopteran Parasitoids

Aspects on the biology and ecology of Anastrepha obliqua, at both laboratory and field conditions, are given. Adult emergence occurred between 11 to 22 days, with an average of 17 days of pupation. In the field, atmospheric relative humidity seems to be the main factor affecting adult emergence, and is independent of soil humidity and/or existence of available host fruits. Both fungus and hymenopteran parasitoids determine that a percentage of pupae never hatch, but another population fraction hatches in small numbers through the following months. Water consumption is important for adult survival on A. obliqua, but water is also a mortality factor when it reared under laboratory conditions. This causes a good number of adult drawings in rearing cages. Spiders also represent an important mortality factor under mass rearing conditions. A. obliqua adults show a high degree of polyphagism and feed on different kinds of ripe fruits different from those which are infested by oviposition of the gravid females. This behavior explains why it is possible to capture A. obliqua in traps placed in plantations of fruits infested by Anastrepha species other than A. obliqua. Sexual maturity is reached after 17 days, and the maximum longevity recorded under laboratory conditions, varied from 40 to 53 days, with a similar survival rate in both sexes. Life expectancy was found to be 29 days for males and 31,5 for females. Marked adults released in the fields were recaptured after 58 days, suggesting a longer expectancy than in laboratory. Under captivity, much of the mating courtship seems to be ignored, and copulation takes place with no major problem, lasting about 45 minutes.

scholarly journals Basic Studies Aiming at Orius minutus (Hemiptera: Anthocoridae) Mass-Rearing

Insects ◽  
2022 ◽  
Vol 13 (1) ◽  
pp. 77
Author(s):  
Hye-Jeong Jun ◽  
Kyoung-Su Kim ◽  
Eun-Hye Ham
Keyword(s):  
Brine Shrimp ◽  
Biological Control Agent ◽  
Mass Rearing ◽  
Control Agent ◽  
Artificial Substrates ◽  
Ephestia Cautella ◽  
Control Group ◽  
Significant Difference ◽  
Oviposition Substrate ◽  
Artificial Oviposition Substrate

This study presented biological and economic data for the mass-rearing of Orius minutus in Korea. Simplifying the mass-rearing process through an alternative diet and an artificial oviposition substrate is a prerequisite for enhancing the usability of this insect as a biological control agent. We compare the hatch rate of O. minutus eggs deposited on a plant substrate with that of eggs deposited on two artificial substrates, cork sheets and rubber. The results indicate that cork sheet is the most cost-effective artificial oviposition substrate for the mass-rearing of O. minutus. We also examine five feeding treatments that included two types of brine shrimp eggs and eggs of Ephestia cautella to compare the number of eggs laid in the fifth generation. We found no significant difference between the two treatment groups; 61.3 eggs were laid in the treatment group fed iron-coated brine shrimp and moth eggs, and 67.4 eggs were laid in the control group. The plant-free model developed in our study can reduce rearing costs by 70.5% compared to the conventional mass-rearing model.

Temperature-dependent development of Oenopia conglobata (Col.: Coccinellidae) fed on Aphis gossypii (Hem.: Aphididae)

2018 ◽  
Vol 38 (04) ◽  
pp. 410-417 ◽  
Author(s):  
Mohammad Amin Jalali ◽  
Samane Sakaki ◽  
Mahdi Ziaaddini ◽  
Kent M. Daane
Keyword(s):  
Development Time ◽  
Developmental Stages ◽  
Aphis Gossypii ◽  
Mass Rearing ◽  
Classical Biological Control ◽  
Biological Cycle ◽  
Temperature Dependent ◽  
Dependent Development ◽  
Thermal Range ◽  
Temperature Thresholds

AbstractLadybeetles are known beneficial insects, with a long history in augmentative and classical biological control. The ladybeetle Oenopia conglobata (L.) is a natural enemy of many herbivores, particularly aphids. The temperature-dependent development of O. conglobata was studied at six constant temperatures (22.5, 25, 27.5, 30, 32.5 and 35 °C) to understand its development rate and environmental constraints better. Linear and nonlinear (Lactin) models were fitted to the data. In the thermal range from 22.5 to 32.5 °C, the rate of development increased for all stages; 35 °C was lethal for all stages and no eggs hatched. The Tb and K values for the biological cycle (egg–adult) were 8.84 °C and 263.15 DD, respectively. Depending on the model, tmin values for the total development time of the coccinellid ranged from 8.45 to 8.82 °C. The nonlinear model of Lactin estimated the optimum and upper temperature thresholds for the total development time of the ladybeetle to be 33.2 and 35.0 °C, respectively. High R2 values and low residual sum of squares values revealed a good fit to the experimental data for total development and different developmental stages of O. conglobata. The results may contribute to the improvement of practical methods for mass rearing of O. conglobata.

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