Pre- and Post-Copulatory Competitiveness of the Genetic Sexing Strain Tapachula-7 of Anastrepha ludens (Diptera: Tephritidae)

2020 ◽  
Vol 113 (5) ◽  
pp. 2163-2170
Author(s):  
Yair Contreras-Navarro ◽  
Diana Pérez-Staples ◽  
Dina Orozco-Dávila ◽  
Francisco Díaz-Fleischer
Keyword(s):  
Sterile Insect Technique ◽  
Copulatory Behavior ◽  
Mass Rearing ◽  
Economic Importance ◽  
Anastrepha Ludens ◽  
Sexual Performance ◽  
Field Cage ◽  
Wild Female ◽  
Genetic Sexing ◽  
Genetic Sexing Strain

Abstract Anastrepha ludens (Loew) (Diptera: Tephritidae) is an endemic pest of Mexico, attacking several fruits of economic importance. The Sterile Insect Technique (SIT), consisting of mass-rearing, irradiation and release of adults, is used to control this pest in affected areas. Currently, a genetic sexing strain (Tapachula 7, Tap-7) consisting only of A. ludens males is being released, yet we lack information on its sexual performance at the early ages when they are released and on its post copulatory behavior in terms of sperm transfer and mating inhibition. Here, sexual competitiveness at young ages and ability to inhibit female re-mating was compared between sterile Tap-7, Standard Bisexual (SB), or wild males both in laboratory and field cage conditions. Sperm stored by females mating with wild, Tap-7, or SB strain males was also compared. Six-day-old sterile Tap-7 males had low mating rates, however, by 7 d of age 80% of males had mated. Tap-7 males were just as likely as wild males to inhibit wild female re-mating. In field cages, sterile Tap-7 males mated faster than wild and sterile males from the SB strain and had comparable mating success to wild males. Females mated to sterile Tap-7 males stored more sperm than those mated to sterile SB males. Females mated to wild males stored more sperm than females mated to any other type of male. Mass-rearing had a greater effect on decreasing sperm stored by females than irradiation. We recommend continuing the release of the GSS strain of A. ludens.

Selection by mating competitiveness improves the performance of Anastrepha ludens males of the genetic sexing strain Tapachula-7

2016 ◽  
Vol 106 (5) ◽  
pp. 624-632 ◽  
Author(s):  
L. Quintero-Fong ◽  
J. Toledo ◽  
L. Ruiz ◽  
P. Rendón ◽  
D. Orozco-Dávila ◽  
...  
Keyword(s):  
Development Time ◽  
Mass Rearing ◽  
Quality Parameters ◽  
Anastrepha Ludens ◽  
Sexual Performance ◽  
Genetic Sexing ◽  
Mating Competitiveness ◽  
Colony Management ◽  
Management Method ◽  
Genetic Sexing Strain

AbstractThe sexual performance of Anastrepha ludens males of the Tapachula-7 genetic sexing strain, produced via selection based on mating success, was compared with that of males produced without selection in competition with wild males. Mating competition, development time, survival, mass-rearing quality parameters and pheromone production were compared. The results showed that selection based on mating competitiveness significantly improved the sexual performance of offspring. Development time, survival of larvae, pupae and adults, and weights of larvae and pupae increased with each selection cycle. Differences in the relative quantity of the pheromone compounds (Z)-3-nonenol and anastrephin were observed when comparing the parental males with the F4 and wild males. The implications of this colony management method on the sterile insect technique are discussed.

scholarly journals A Novel Genetic Sexing Strain of Anastrepha Ludens for Cost-Effective Sterile Insect Technique Applications: Improved Genetic Stability and Rearing Efficiency

Insects ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 499
Author(s):  
Edwin Ramírez-Santos ◽  
Pedro Rendon ◽  
Georgia Gouvi ◽  
Antigone Zacharopoulou ◽  
Kostas Bourtzis ◽  
...  
Keyword(s):  
Genetic Stability ◽  
Sterile Insect Technique ◽  
Production Efficiency ◽  
Small Scale ◽  
Management Approach ◽  
Anastrepha Ludens ◽  
Male Mating ◽  
Pest Species ◽  
Genetic Sexing ◽  
Genetic Sexing Strain

Anastrepha ludens (Loew) is one of the most destructive insect pests damaging several fruits of economic importance. The sterile insect technique (SIT) is used under an area-wide integrated pest management approach, to suppress these pest populations. Mass rearing facilities were initially established to produce sterile males of bi-sexual strains in support of SIT. The first genetic sexing strain (GSS) for A. ludens, Tapachula-7, based on pupal color dimorphism, was a key development since the release of males-only significantly increases the SIT efficiency. In this study, we document the development of a novel pupal color-based GSS. Twelve radiation-induced translocation lines were assessed as potential GSS in terms of recombination rates and rearing efficiency at a small scale. The best one, GUA10, was cytogenetically characterized: it was shown to carry a single translocation between the Y chromosome and chromosome 2, which is known to carry the black pupae marker. This GSS was further evaluated at medium and large scales regarding its genetic stability, productivity and quality versus Tapachula-7. GUA10 presented better genetic stability, fecundity, fertility, production efficiency, flying ability, and male mating, clear indicators that GUA10 GSS can significantly improve the efficacy and cost-effectiveness of SIT applications against this pest species.

scholarly journals Pupation Substrate Type and Volume Affect Pupation, Quality Parameters and Production Costs of a Reproductive Colony of Ceratitis capitata (Diptera: Tephritidae) VIENNA 8 Genetic Sexing Strain

Insects ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 337
Author(s):  
Carlos Pascacio-Villafán ◽  
Luis Quintero-Fong ◽  
Larissa Guillén ◽  
José Pedro Rivera-Ciprian ◽  
Reynaldo Aguilar ◽  
...  
Keyword(s):  
Wheat Bran ◽  
Ceratitis Capitata ◽  
Mass Rearing ◽  
Physicochemical Characteristics ◽  
Production Costs ◽  
Cellulose I ◽  
Coconut Fiber ◽  
Genetic Sexing ◽  
Recycled Paper ◽  
Genetic Sexing Strain

Adequate pupation substrates and substrate volume are critical factors in the mass-rearing of insects for Sterile Insect Technique (SIT) applications. To identify an ideal pupation substrate for a reproductive colony of Ceratitis capitata (Wiedemann) VIENNA 8 genetic sexing strain, we first examined pupation in cellulose from recycled paper (cellulose I), sawdust, fine wheat bran, vermiculite and coconut fiber using a volume of 2.5–12.5 mL of substrate for each 5 mL volume of fly larvae. We found a positive relationship between substrate volume and pupation, with cellulose I generating the highest proportions of pupation and coconut fiber the lowest. Higher proportions of female flies (white pupae) pupated in sawdust. The proportion of female fliers increased as substrate volume rose in sawdust and coconut fiber, whereas it decreased in vermiculite and cellulose. In a second experiment, we tested three types of cellulose differing in physicochemical characteristics (celluloses I, II and III), sawdust, and fine wheat bran using a substrate:larvae ratio of 1:1. The three types of cellulose produced the highest pupation levels. The highest proportions of female fliers were observed in sawdust, and cellulose types III and II. Cellulose III and sawdust at relatively low volumes were more cost-effective to produce one million pupae than other substrates, including fine wheat bran used in a mass-rearing facility in Mexico.

Female pupae of the genetic sexing strain “Tap-7” of Anastrepha ludens as hosts of Coptera haywardi

BioControl ◽  
2013 ◽  
Vol 59 (2) ◽  
pp. 149-157 ◽  
Author(s):  
Florida López-Arriaga ◽  
Pablo Montoya ◽  
Jorge Cancino ◽  
Jorge Toledo ◽  
Pablo Liedo
Keyword(s):  
Anastrepha Ludens ◽  
Genetic Sexing ◽  
Genetic Sexing Strain

scholarly journals Quantification and Impact of Cold Storage and Heat Exposure on Mass Rearing Program of Bactrocera dorsalis (Diptera:Tephritidae) Genetic Sexing Strain

Insects ◽  
2020 ◽  
Vol 11 (11) ◽  
pp. 821
Author(s):  
Jia Lin ◽  
Hanano Yamada ◽  
Ningfeng Lu ◽  
Guofu Ao ◽  
Weiwei Yuan ◽  
...  
Keyword(s):  
Cold Storage ◽  
Heat Exposure ◽  
Thermal Exposure ◽  
Mass Rearing ◽  
Bactrocera Dorsalis ◽  
Short Term ◽  
Genetic Sexing ◽  
Emergence Rate ◽  
Genetic Sexing Strain ◽  
The Impact

Cold storage and heat exposure are crucial components of tephritid fruit fly mass-rearing programs, as they influence the development and fitness traits of produced flies. This work investigated the effects of cold storage on the pupal developmental parameters and quality of Bactrocera dorsalis (Hendel) genetic sexing strain (GSS) adults. Furthermore, the impact of short-term thermal exposure on the fecundity of B. dorsalis (GSS) that also underwent pupal cold storage was examined. Our results show that pupal development time, emergence rate, partial emergence rate, flight ability and fecundity were significantly affected by low temperature and pupal age and their interaction. Pupal cold storage did not pose negative impacts on the mating competition and response to methyl eugenol (ME) in the males. In addition, compared with the adults that were subjected to the same pupal storage protocol (five-day-old pupae stored at 13 °C), adult exposure to 41 °C for 1 h showed significant reparative effects on fecundity. In summary, the cold storage procedure of B. dorsalis (GSS) pupae has the potential to improve the flexibility and efficiency of mass-rearing schedules. Furthermore, short-term thermal exposure showed reparative effects on the fecundity costs induced by pupal cold storage in B. dorsalis (GSS).

When Less Is More: Sex Ratios for the Mass-Rearing of Anastrepha ludens (Diptera: Tephritidae)

2019 ◽  
Author(s):  
G I González-López ◽  
E Solís-Echeverría ◽  
F Díaz-Fleischer ◽  
D Pérez-Staples
Keyword(s):  
Sex Ratio ◽  
Egg Production ◽  
Sex Ratios ◽  
Pupal Stage ◽  
Mass Rearing ◽  
Anastrepha Ludens ◽  
Adult Density ◽  
Genetic Sexing ◽  
Male Harassment ◽  
Less Is More

Abstract Anastrepha ludens (Loew) is one of the most important pests of citrus and mango crops in Mexico. A method used to control this pest is the sterile insect technique, which consists in the mass production, irradiation, and release of insects in affected areas. The production of insects begins with the establishment of colonies to produce eggs, which must be highly fertile to ensure an adequate production of larvae. However, female fecundity and fertility can be affected by adult density and sex ratio, thus an optimal sex ratio in mass-rearing cages must be used. The genetic sexing strain of A. ludens (Tapachula-7) allows the identification of the sex at the pupal stage, making it possible to establish rearing cages with different sex ratios. We determined if different sex ratios have an effect on egg production. Two sex ratios (4♀: 1♂ and 1♀: 1♂) were compared. Fecundity, fertility and survival at different ages were also determined. Higher fertility and fecundity per female were observed at a ratio of 4:1. However, females with higher fecundity had reduced survival probabilities. In conclusion, maintaining colonies with a lower proportion of males in cages ensures a greater fecundity and fertility. Further research is necessary to understand whether results can be attributed to lower male harassment in cages.

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