Genome‐wide DNA‐methylation profiles in human bone marrow mesenchymal stem cells on titanium surfaces

2019 ◽  
Vol 127 (3) ◽  
pp. 196-209
Author(s):  
Mingyue Lyu ◽  
Yunfei Zheng ◽  
Lingfei Jia ◽  
Yan Zheng ◽  
Yanping Liu ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Genome Wide ◽  
Marrow Mesenchymal Stem Cells ◽  
Titanium Surfaces

scholarly journals The Effect of Culture on Human Bone Marrow Mesenchymal Stem Cells: Focus on DNA Methylation Profiles

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Angela Bentivegna ◽  
Gaia Roversi ◽  
Gabriele Riva ◽  
Laura Paoletta ◽  
Serena Redaelli ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Differentiation Potential ◽  
Marrow Mesenchymal Stem Cells

Human bone marrow mesenchymal stem cells (hBM-MSCs) are the best characterized multipotent adult stem cells. Their self-renewal capacity, multilineage differentiation potential, and immunomodulatory properties have indicated that they can be used in many clinical therapies. In a previous work we studied the DNA methylation levels of hBM-MSC genomic DNA in order to delineate a kind of methylation signature specific for early and late passages of culture. In the present work we focused on the modification of the methylation profiles of the X chromosome and imprinted loci, as sites expected to be more stable than whole genome. We propose a model where cultured hBM-MSCs undergo random modifications at the methylation level of most CGIs, nevertheless reflecting the original methylation status. We also pointed out global genome-wide demethylation connected to the long-term culture and senescence. Modification at CGIs promoters of specific genes could be related to the decrease in adipogenic differentiation potential. In conclusion, we showed important changes in CGIs methylation due to long-termin vitroculture that may affect the differentiation potential of hBM-MSCs. Therefore it is necessary to optimize the experimental conditions forin vitroexpansion in order to minimize these epigenetic changes and to standardize safer procedures.

scholarly journals Genome-scale DNA methylation pattern profiling of human bone marrow mesenchymal stem cells in long-term culture

2012 ◽  
Vol 44 (8) ◽  
pp. 503 ◽  
Author(s):  
Mi Ran Choi ◽  
Yong-Ho In ◽  
Jungsun Park ◽  
Taesung Park ◽  
Kyoung Hwa Jung ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Methylation Pattern ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Marrow Mesenchymal Stem Cells ◽  
Genome Scale

Biological response of human bone marrow mesenchymal stem cells to fluoride-modified titanium surfaces

2010 ◽  
Vol 21 (11) ◽  
pp. 1234-1241 ◽  
Author(s):  
Luigi Guida ◽  
Marco Annunziata ◽  
Antonio Rocci ◽  
Maria Contaldo ◽  
Rosario Rullo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Biological Response ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Marrow Mesenchymal Stem Cells ◽  
Titanium Surfaces

Candidate kinases for adipogenesis and osteoblastogenesis from human bone marrow mesenchymal stem cells

2021 ◽  
Author(s):  
Xia Yi ◽  
Ping Wu ◽  
Jianyun Liu ◽  
Shan He ◽  
Ying Gong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Transcription Factors ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Cytoplasmic Proteins ◽  
Marrow Mesenchymal Stem Cells

Adipogenesis and osteoblastogenesis (adipo-osteoblastogenesis) are closely related processes involving with the phosphorylation of numerous cytoplasmic proteins and key transcription factors.

Comparative analysis of human bone marrow mesenchymal stem cells, articular cartilage derived chondroprogenitors and chondrocytes to determine cell superiority for cartilage regeneration

2021 ◽  
Vol 123 (4) ◽  
pp. 151713
Author(s):  
Elizabeth Vinod ◽  
Roshni Parameswaran ◽  
Soosai Manickam Amirtham ◽  
Grace Rebekah ◽  
Upasana Kachroo
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Comparative Analysis ◽  
Articular Cartilage ◽  
Cartilage Regeneration ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Marrow Mesenchymal Stem Cells

The red-light emitting diode irradiation increases proliferation of human bone marrow mesenchymal stem cells preserving their immunophenotype

2021 ◽  
pp. 1-11
Author(s):  
Rafał B. Lewandowski ◽  
Małgorzata Stępińska ◽  
Andrzej Gietka ◽  
Monika Dobrzyńska ◽  
Mariusz P. Łapiński ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Light Emitting Diode ◽  
Red Light ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Light Emitting ◽  
Marrow Mesenchymal Stem Cells

Inner ear progenitor cells can be generated in vitro from human bone marrow mesenchymal stem cells

2012 ◽  
Vol 7 (6) ◽  
pp. 757-767 ◽  
Author(s):  
Sarah L Boddy ◽  
Wei Chen ◽  
Ricardo Romero-Guevara ◽  
Lucksy Kottam ◽  
Illaria Bellantuono ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Inner Ear ◽  
Progenitor Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Marrow Mesenchymal Stem Cells

Shrinkage-free preparation of scaffold-free cartilage-like disk-shaped cell sheet using human bone marrow mesenchymal stem cells

2011 ◽  
Vol 111 (4) ◽  
pp. 489-492 ◽  
Author(s):  
Satoshi Maeda ◽  
Takashi Fujitomo ◽  
Takahiro Okabe ◽  
Shigeyuki Wakitani ◽  
Mutsumi Takagi
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Cell Sheet ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Shaped Cell ◽  
Marrow Mesenchymal Stem Cells
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