Migration dynamics of an important rice pest: The brown planthopper (
            Nilaparvata lugens
            ) across Asia—Insights from population genomics

AbstractBrown planthoppers (Nilaparvata lugens) are the most serious insect pests of rice, one of the world’s most important staple crops. They reproduce year-round in the tropical parts of their distribution, but cannot overwinter in the temperate areas where they occur, and invade seasonally from elsewhere. Decades of research has not revealed their source unambiguously. We therefore sequenced the genomes of brown planthopper populations from across temperate and tropical parts of their distribution and show that the Indochinese peninsula is the major source of migration into temperate China. The Philippines, once considered a key source, is not significant, with little evidence for their migration into China. We find support for immigration from the west of China contributing to these regional dynamics. The lack of connectivity between the Philippines and mainland China explains the different evolution of Imidacloprid resistance in these populations. This study highlights the promise of whole genome sequence data to understand migration when gene flow is high – a situation that has been difficult to resolve using traditional genetic markers.

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Genomic and transcriptomic insights into the cytochrome P450 monooxygenase gene repertoire in the rice pest brown planthopper,Nilaparvata lugens

10.1603/ice.2016.108969 ◽

2016 ◽

Author(s):

Yanyuan Bao

Keyword(s):

Cytochrome P450 ◽

Brown Planthopper ◽

Nilaparvata Lugens ◽

Cytochrome P450 Monooxygenase ◽

Gene Repertoire ◽

P450 Monooxygenase ◽

Monooxygenase Gene ◽

Rice Pest

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Genomic and transcriptomic insights into the cytochrome P450 monooxygenase gene repertoire in the rice pest brown planthopper, Nilaparvata lugens

Genomics ◽

10.1016/j.ygeno.2015.07.010 ◽

2015 ◽

Vol 106 (5) ◽

pp. 301-309 ◽

Cited By ~ 30

Author(s):

Shu-Hua Lao ◽

Xiao-Hui Huang ◽

Hai-Jian Huang ◽

Cheng-Wen Liu ◽

Chuan-Xi Zhang ◽

...

Keyword(s):

Cytochrome P450 ◽

Brown Planthopper ◽

Nilaparvata Lugens ◽

Cytochrome P450 Monooxygenase ◽

Gene Repertoire ◽

P450 Monooxygenase ◽

Monooxygenase Gene ◽

Rice Pest

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HSP70/DNAJ Family of Genes in the Brown Planthopper, Nilaparvata lugens: Diversity and Function

Genes ◽

10.3390/genes12030394 ◽

2021 ◽

Vol 12 (3) ◽

pp. 394

Author(s):

Xuan Chen ◽

Ze-Dong Li ◽

Dan-Ting Li ◽

Ming-Xing Jiang ◽

Chuan-Xi Zhang

Keyword(s):

Phylogenetic Analyses ◽

Brown Planthopper ◽

Gene Families ◽

Growth Conditions ◽

Nilaparvata Lugens ◽

Biological Processes ◽

Temporal Expression ◽

Rice Pest ◽

Spatio Temporal ◽

And Function

Heat shock 70kDa proteins (HSP70s) and their cochaperones DNAJs are ubiquitous molecular chaperones, which function as the “HSP70/DNAJ machinery” in a myriad of biological processes. At present, a number of HSP70s have been classified in many species, but studies on DNAJs, especially in insects, are lacking. Here, we first systematically identified and characterized the HSP70 and DNAJ family members in the brown planthopper (BPH), Nilaparvata lugens, a destructive rice pest in Asia. A total of nine HSP70 and 31 DNAJ genes were identified in the BPH genome. Sequence and phylogenetic analyses revealed the high diversity of the NlDNAJ family. Additionally, spatio-temporal expression analysis showed that most NlHSP70 and NlDNAJ genes were highly expressed in the adult stage and gonads. Furthermore, RNA interference (RNAi) revealed that seven NlHSP70s and 10 NlDNAJs play indispensable roles in the nymphal development, oogenesis, and female fertility of N. lugens under physiological growth conditions; in addition, one HSP70 (NlHSP68) was found to be important in the thermal tolerance of eggs. Together, our results in this study shed more light on the biological roles of HSP70/DNAJ in regulating life cycle, coping with environmental stresses, and mediating the interactions within, or between, the two gene families in insects.

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