scholarly journals HSP70/DNAJ Family of Genes in the Brown Planthopper, Nilaparvata lugens: Diversity and Function

Genes ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 394
Author(s):  
Xuan Chen ◽  
Ze-Dong Li ◽  
Dan-Ting Li ◽  
Ming-Xing Jiang ◽  
Chuan-Xi Zhang
Keyword(s):  
Phylogenetic Analyses ◽  
Brown Planthopper ◽  
Gene Families ◽  
Growth Conditions ◽  
Nilaparvata Lugens ◽  
Biological Processes ◽  
Temporal Expression ◽  
Rice Pest ◽  
Spatio Temporal ◽  
And Function

Heat shock 70kDa proteins (HSP70s) and their cochaperones DNAJs are ubiquitous molecular chaperones, which function as the “HSP70/DNAJ machinery” in a myriad of biological processes. At present, a number of HSP70s have been classified in many species, but studies on DNAJs, especially in insects, are lacking. Here, we first systematically identified and characterized the HSP70 and DNAJ family members in the brown planthopper (BPH), Nilaparvata lugens, a destructive rice pest in Asia. A total of nine HSP70 and 31 DNAJ genes were identified in the BPH genome. Sequence and phylogenetic analyses revealed the high diversity of the NlDNAJ family. Additionally, spatio-temporal expression analysis showed that most NlHSP70 and NlDNAJ genes were highly expressed in the adult stage and gonads. Furthermore, RNA interference (RNAi) revealed that seven NlHSP70s and 10 NlDNAJs play indispensable roles in the nymphal development, oogenesis, and female fertility of N. lugens under physiological growth conditions; in addition, one HSP70 (NlHSP68) was found to be important in the thermal tolerance of eggs. Together, our results in this study shed more light on the biological roles of HSP70/DNAJ in regulating life cycle, coping with environmental stresses, and mediating the interactions within, or between, the two gene families in insects.

scholarly journals Spatio-temporal expression of Prospero is finely tuned to allow the correct development and function of the nervous system in Drosophila melanogaster

2007 ◽  
Vol 304 (1) ◽  
pp. 62-74 ◽  
Author(s):  
Laure Guenin ◽  
Yaël Grosjean ◽  
Stéphane Fraichard ◽  
Angel Acebes ◽  
Fawzia Baba-Aissa ◽  
...  
Keyword(s):  
Nervous System ◽  
Drosophila Melanogaster ◽  
Temporal Expression ◽  
Spatio Temporal ◽  
And Function

scholarly journals Identification and Characterization of Three Heat Shock Protein 90 (Hsp90) Homologs in the Brown Planthopper

Genes ◽  
2020 ◽  
Vol 11 (9) ◽  
pp. 1074
Author(s):  
Xuan Chen ◽  
Ze-Dong Li ◽  
Yi-Ting Dai ◽  
Ming-Xing Jiang ◽  
Chuan-Xi Zhang
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Developmental Stage ◽  
Brown Planthopper ◽  
Evolutionary Relationship ◽  
Heat Shock Protein 90 ◽  
Transcript Level ◽  
Growth Conditions ◽  
Nilaparvata Lugens ◽  
Early Developmental Stage

Hsp90 (heat shock protein 90) chaperone machinery is considered to be a key regulator of proteostasis under both physiological and stress growth conditions in eukaryotic cells. The high conservation of both the sequence and function of Hsp90 allows for the utilization of various species to explore new phenotypes and mechanisms. In this study, three Hsp90 homologs were identified in the brown planthopper (BPH), Nilaparvata lugens: cytosolic NlHsp90, endoplasmic reticulum (ER) NlGRP94 and mitochondrial NlTRAP1. Sequence analysis and phylogenetic construction showed that these proteins belonged to distinct classes consistent with the predicted localization and suggested an evolutionary relationship between NlTRAP1 and bacterial HtpG (high-temperature protein G). Temporospatial expression analyses showed that NlHsp90 was inducible under heat stress throughout the developmental stage, while NlGRP94 was only induced at the egg stage. All three genes had a significantly high transcript level in the ovary. The RNA interference-mediated knockdown of NlHsp90 its essential role in nymph development and oogenesis under physiological conditions. NlGRP94 was also required during the early developmental stage and played a crucial role in oogenesis, fecundity and late embryogenesis. Notably, we first found that NlHsp90 and NlGRP94 were likely involved in the cuticle structure of female BPH. Together, our research revealed multifunctional roles of Hsp90s in the BPH.

scholarly journals The Effect of Antibiotic Treatment on the Bacterial Community of the Brown Planthopper and Its Correlation with Rice Virulence

Agronomy ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 2327
Author(s):  
Xiaorong Xu ◽  
Liang Chen ◽  
Hantao Zhou ◽  
Ming Tang
Keyword(s):  
Bacterial Community ◽  
Bacterial Communities ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Biological Processes ◽  
Rna Seq ◽  
Phenylpropanoid Biosynthesis ◽  
And Control ◽  
Selection Of

The prevention and control of planthoppers represent important issues for rice production. Current long-term control methods rely on pesticides, which raise concerns about environmental pollution. Recently, evidence has suggested that bacterial symbionts are important factors influencing the formation of Hemiptera insect biotypes and the selection of host plants for insects, which suggesting that targeting bacterial communities may be an effective alternative method for planthopper control. In this study, we perturbed the bacterial communities of the brown planthopper, Nilaparvata lugens, by feeding antibiotic-treated rice and used RNA-seq to examine the transcriptome of normal rice fed with perturbed BPHs by RNA-seq. Our results showed that the composition of the bacterial communities significantly changed after the perturbation, which was accompanied by changes in distinct biological processes of rice, especially the phenylpropanoid biosynthesis pathway, compared with the effect of the BPH feeding on rice without bacterial communities perturbation. Our work establishes a protocol for bacterial communities perturbation in BPH, demonstrating the link between bacterial community and the responses to BPH feeding and providing new insights into the interaction between BPH and rice.

scholarly journals Migration dynamics of an important rice pest: The brown planthopper ( Nilaparvata lugens ) across Asia—Insights from population genomics

2020 ◽  
Vol 13 (9) ◽  
pp. 2449-2459 ◽  
Author(s):  
James P. Hereward ◽  
Xuhong Cai ◽  
Ambrocio Melvin A. Matias ◽  
Gimme H. Walter ◽  
Chenxi Xu ◽  
...  
Keyword(s):  
Population Genomics ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Migration Dynamics ◽  
Rice Pest

scholarly journals Dissimilar evolutionary histories of two resistance gene families in the genus Solanum

Genome ◽  
2017 ◽  
Vol 60 (1) ◽  
pp. 17-25
Author(s):  
Diana María Segura ◽  
Ricardo Williams Masuelli ◽  
M. Virginia Sanchez-Puerta
Keyword(s):  
Resistance Gene ◽  
Phylogenetic Analyses ◽  
Gene Families ◽  
Tomato Genome ◽  
Potato Genome ◽  
Replication Errors ◽  
Gene Copies ◽  
Genomic Analyses ◽  
Plant Resistance Gene ◽  
And Function

Genomic analyses have shown that most genes in eukaryotic lineages belong to families. Gene families vary in terms of number of members, nucleotide similarity, gene integrity, expression, and function. Often, the members of gene families are arranged in clusters, which contribute to maintaining similarity among gene copies and also to generate duplicates through replication errors. Gene families offer us an opportunity to examine the forces involved in the evolution of the genomes and to study recombination events and genomic rearrangements. In this work, we focused on the evolution of two plant resistance gene families, Sw5 and Mi-1, and analyzed the completely sequenced nuclear genomes of potato and tomato. We first noticed that the potato genome carries larger resistance gene families than tomato, but all gene copies are pseudogenes. Second, phylogenetic analyses indicated that Sw5 and Mi-1 gene families had dissimilar evolutionary histories. In contrast to Sw5, Mi-1 homologues suffered repeated gene conversion events among the gene copies, particularly in the tomato genome.

The Spatio-Temporal Expression Profiles of CD4 + T Cell Differentiation and Function-Related Genes During EAE Pathogenesis

Inflammation ◽  
2016 ◽  
Vol 40 (1) ◽  
pp. 195-204 ◽  
Author(s):  
Yingying Cai ◽  
Hu Shen ◽  
Chaoyan Qin ◽  
Jinfeng Zhou ◽  
Weiming Lai ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
T Cell ◽  
Expression Profiles ◽  
T Cell Differentiation ◽  
Cd4 T Cell ◽  
Temporal Expression ◽  
Spatio Temporal ◽  
And Function

cDNA Cloning, Heterogeneous Expression and Biochemical Characterization of a Novel Trypsin-Like Protease from Nilaparvata lugens

2010 ◽  
Vol 65 (1-2) ◽  
pp. 109-118 ◽  
Author(s):  
Zhifan Yang ◽  
Xunli Xia ◽  
Xiaoxue Wang ◽  
Guangyuan He
Keyword(s):  
Biochemical Characterization ◽  
Brown Planthopper ◽  
Gene Families ◽  
Nilaparvata Lugens ◽  
Maximum Activity ◽  
Protease Gene ◽  
Rice Varieties ◽  
Reading Frame ◽  
Digestive Proteases ◽  
Heterogeneous Expression

A reverse transcription-polymerase chain reaction (RT-PCR) strategy was used to clone diverse trypsin-like protease gene transcripts from midguts of the brown planthopper Nilaparvata lugens Stål (Homoptera: Delphacidae). Six individual trypsin-like protease transcripts were identifi ed. On the basis of one nucleotide sequence of the six clones, a full-length cDNA sequence (1902 bp) was obtained by rapid amplifi cation of cDNA ends (RACE). The cDNA contained an 1128-bp open reading frame encoding a putative protein of 375 amino acids with typical features of the trypsin-like protease. Heterogeneous expression of the coding sequence for the mature peptide in Escherichia coli cells showed that the expressed protease with a molecular weight of 27.0 is active, for its BApNAse activity assayed by using BApNA (N-benzoyl-D,L-arginine-p-nitroanilide) as substrate. The protease had its maximum activity at pH 8.0 and 35 °C. A much better stability was observed at pH values above 4.0 and temperatures below 40 °C. The enzyme was strongly inhibited by serine protease inhibitor. The trypsin-like protease is therefore likely one of the major digestive proteases responsible for protein hydrolysis in N. lugens gut, and multiple gene families encoding digestive proteases may help in adaptation of this sap-sucker to different rice varieties.

scholarly journals Peroxiredoxin alleviates the fitness costs of imidacloprid resistance in an insect pest of rice

PLoS Biology ◽  
2021 ◽  
Vol 19 (4) ◽  
pp. e3001190
Author(s):  
Rui Pang ◽  
Ke Xing ◽  
Longyu Yuan ◽  
Zhikun Liang ◽  
Meng Chen ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Insect Pest ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Fitness Cost ◽  
Ros Scavenging ◽  
Fitness Costs ◽  
Effective Measure ◽  
And Function ◽  
The Cost

Chemical insecticides have been heavily employed as the most effective measure for control of agricultural and medical pests, but evolution of resistance by pests threatens the sustainability of this approach. Resistance-conferring mutations sometimes impose fitness costs, which may drive subsequent evolution of compensatory modifier mutations alleviating the costs of resistance. However, how modifier mutations evolve and function to overcome the fitness cost of resistance still remains unknown. Here we show that overexpression of P450s not only confers imidacloprid resistance in the brown planthopper, Nilaparvata lugens, the most voracious pest of rice, but also leads to elevated production of reactive oxygen species (ROS) through metabolism of imidacloprid and host plant compounds. The inevitable production of ROS incurs a fitness cost to the pest, which drives the increase or fixation of the compensatory modifier allele T65549 within the promoter region of N. lugens peroxiredoxin (NlPrx) in the pest populations. T65549 allele in turn upregulates the expression of NlPrx and thus increases resistant individuals’ ability to clear the cost-incurring ROS of any source. The frequent involvement of P450s in insecticide resistance and their capacity to produce ROS while metabolizing their substrates suggest that peroxiredoxin or other ROS-scavenging genes may be among the common modifier genes for alleviating the fitness cost of insecticide resistance.

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