Lactate Transport in Red Blood Cells by Monocarboxylate Transporter MCT1 and Its Accessory Protein CD147 In Brazilian Sport Horses of Different Performance Levels

The distribution of monocarboxylate transporter (MCT) isoforms 1 and 4, which mediate the plasmalemmal transport of l-lactic and pyruvic acids, has been identified in the placentae of rats and rabbits at different ages of gestation. Groups of three pregnant Sprague-Dawley rats and New Zealand White rabbits were sacrificed on gestation days (GD) 11, 14, 18, or 20 and on GD 13, 18, or 28, respectively. Placentae were removed and processed for immunohistochemical detection of MCT1 and MCT4. In the rat, staining for MCT1 was associated with lakes and blood vessels containing enucleated red blood cells (maternal vessels) while staining for MCT4 was associated with vessels containing nucleated red blood cells (embryofoetal vessels). In the rabbit, staining for MCT1 was associated with blood vessels containing nucleated red blood cells while staining for MCT4 was associated with vessels containing enucleated red blood cells. Strength of staining for MCT1 decreased during gestation in both species, but that for MCT4 was stronger than that for MCT1 and was consistent between gestation days. The results imply an opposite polarity of MCT1 and MCT4 across the trophoblast between rat and rabbit.

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A mathematical model for lactate transport to red blood cells

The Journal of Physiological Sciences ◽

10.1007/s12576-010-0125-8 ◽

2010 ◽

Vol 61 (2) ◽

pp. 93-102 ◽

Cited By ~ 4

Author(s):

Patrick Wahl ◽

Zengyuan Yue ◽

Christoph Zinner ◽

Wilhelm Bloch ◽

Joachim Mester

Keyword(s):

Mathematical Model ◽

Red Blood Cells ◽

Blood Cells ◽

Lactate Transport

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Lactate transport in red blood cells by monocarboxylate transporters

Equine Veterinary Journal ◽

10.1111/j.2042-3306.2002.tb05482.x ◽

2002 ◽

Vol 34 (S34) ◽

pp. 555-559 ◽

Cited By ~ 26

Author(s):

N. M. KOHO ◽

L. K. VÄIHKÖNEN ◽

A. R. PÖSÖ

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

Monocarboxylate Transporters ◽

Lactate Transport

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Interindividual variation in total and carrier-mediated lactate influx into red blood cells

AJP Regulatory Integrative and Comparative Physiology ◽

10.1152/ajpregu.1998.274.4.r1025 ◽

1998 ◽

Vol 274 (4) ◽

pp. R1025-R1030 ◽

Cited By ~ 8

Author(s):

Leena K. Väihkönen ◽

A. Reeta Pösö

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

Venous Blood ◽

Age Groups ◽

Lactate Concentration ◽

Bimodal Distribution ◽

Monocarboxylate Transporter ◽

Interindividual Variation ◽

Transport Activity ◽

Major Carrier

To study in standardbred horses interindividual variation in the influx of lactate into red blood cells, venous blood samples were collected from 89 horses from 2 wk to 9 yr of age. For 62 horses, the rate of influx was normally distributed with a mean rate of 4.09 nmol ⋅ mg protein−1 ⋅ min−1at a lactate concentration of 10 mM, and the respective value for the other 27 horses was 0.58 nmol ⋅ mg protein−1 ⋅ min−1. At 30 mM of lactate, the rates were 8.71 and 1.97 nmol ⋅ mg protein−1 ⋅ min−1, respectively. This bimodal distribution was independent of age. In horses with high transport activity, the monocarboxylate transporter (MCT) appears to be the major carrier, whereas, in those with low transport activity, no activity of the MCT could be detected. The band 3 protein may account for 18–39% of transport activity. With all age groups combined, the transport activity tended to be higher in mares than in stallions. Lactate transport into red blood cells seems thus to be an inherent property in which participation of various transporters varies interindividually.

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Lactate-transport activity in RBCs of trained and untrained individuals from four racing species

AJP Regulatory Integrative and Comparative Physiology ◽

10.1152/ajpregu.2001.281.1.r19 ◽

2001 ◽

Vol 281 (1) ◽

pp. R19-R24 ◽

Cited By ~ 12

Author(s):

Leena K. Väihkönen ◽

Olli J. Heinonen ◽

Seppo Hyyppä ◽

Mauri Nieminen ◽

A. Reeta Pösö

Keyword(s):

Blood Cells ◽

Band 3 ◽

Monocarboxylate Transporter ◽

Band 3 Protein ◽

Transport Activity ◽

Lactate Transport ◽

Inorganic Anion ◽

Blood Samples ◽

Sled Dogs ◽

And Performance

In red blood cells (RBC) of horses, both lactate-transport activity and lactate accumulation during races vary interindividually. To study whether similar variation in lactate transport is apparent also in RBCs of other racing species, blood samples were collected from 21 reindeer, 40 horses, 31 humans, and 38 dogs. Total lactate-transport activity was measured at 10 and 30 mM concentrations, and the roles of the monocarboxylate-transporter (MCT) and the inorganic anion-exchange transporter (band-3 protein) were studied with inhibitors. In the reindeer and in one-third of the horses, lactate transport was low and mediated mainly by band-3 protein and nonionic diffusion. In the humans, dogs, and the remaining two-thirds of the horses, lactate transport was high and MCT was the main transporter. No correlation existed between MCT activity and the athleticism of the species. In the horses and humans, training had no effect on lactate transport, but in the reindeer and sled dogs, training increased total lactate transport. These results show that among the racing species studied, only in horses was the distribution of lactate-transport activity bimodal, and the possible connection between RBC lactate and performance capacity, especially in this species, warrants further studies.

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