Lactate-transport activity in RBCs of trained and untrained individuals from four racing species

2001 ◽  
Vol 281 (1) ◽  
pp. R19-R24 ◽  
Author(s):  
Leena K. Väihkönen ◽  
Olli J. Heinonen ◽  
Seppo Hyyppä ◽  
Mauri Nieminen ◽  
A. Reeta Pösö
Keyword(s):  
Blood Cells ◽  
Band 3 ◽  
Monocarboxylate Transporter ◽  
Band 3 Protein ◽  
Transport Activity ◽  
Lactate Transport ◽  
Inorganic Anion ◽  
Blood Samples ◽  
Sled Dogs ◽  
And Performance

In red blood cells (RBC) of horses, both lactate-transport activity and lactate accumulation during races vary interindividually. To study whether similar variation in lactate transport is apparent also in RBCs of other racing species, blood samples were collected from 21 reindeer, 40 horses, 31 humans, and 38 dogs. Total lactate-transport activity was measured at 10 and 30 mM concentrations, and the roles of the monocarboxylate-transporter (MCT) and the inorganic anion-exchange transporter (band-3 protein) were studied with inhibitors. In the reindeer and in one-third of the horses, lactate transport was low and mediated mainly by band-3 protein and nonionic diffusion. In the humans, dogs, and the remaining two-thirds of the horses, lactate transport was high and MCT was the main transporter. No correlation existed between MCT activity and the athleticism of the species. In the horses and humans, training had no effect on lactate transport, but in the reindeer and sled dogs, training increased total lactate transport. These results show that among the racing species studied, only in horses was the distribution of lactate-transport activity bimodal, and the possible connection between RBC lactate and performance capacity, especially in this species, warrants further studies.

Lactate influx into red blood cells of athletic and nonathletic species

1995 ◽  
Vol 268 (5) ◽  
pp. R1121-R1128 ◽  
Author(s):  
M. S. Skelton ◽  
D. E. Kremer ◽  
E. W. Smith ◽  
L. B. Gladden
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Bos Taurus ◽  
Canis Familiaris ◽  
Band 3 ◽  
Capra Hircus ◽  
Disulfonic Acid ◽  
Inorganic Anion ◽  
Blood Samples ◽  
Carrier Mechanism

Transport of lactate across the erythrocyte membrane proceeds by three distinct pathways: 1) nonionic diffusion of lactic acid, 2) inorganic anion exchange (band 3), and 3) a monocarboxylate-specific (MC) carrier mechanism. This study determined the contributions of these three pathways in the red blood cells (RBCs) of “athletic” and “nonathletic” species. Blood samples were obtained from four male animals of each species: 1) Canis familiaris (dogs), 2) Capra hircus (goats), 3) Equus caballus (horses), and 4) Bos taurus (cattle). Contribution of each pathway to total lactate influx was determined by measuring L-[14C]lactate influx into lactate-depleted control RBCs, p-chloromercuribenzenesulfonic acid (PCMBS)-treated (1 mM) RBCs, and 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS)-treated (0.2 mM) RBCs at three lactate concentrations ([La] values 1.6, 8.1, and 41 mM). PCMBS blocked MC transport and DIDS blocked the band 3 pathway. Lactate influx into the RBCs of the athletic species was 4-160 times faster (P < 0.05) than influx into the RBCs of the nonathletic species at 8.1 and 41 mM [La] values. Nonionic diffusion was greater in the RBCs of nonathletic animals (approximately 7-25%) than in the RBCs of athletic animals (approximately 4%). A significantly higher percentage of the total lactate influx occurred via the band 3 system in the RBCs from the nonathletic animals (approximately 56-83%) vs. the RBCs from the athletic animals (approximately 6-7%) at all [La] values.(ABSTRACT TRUNCATED AT 250 WORDS)

Faster lactate transport across red blood cell membrane in sickle cell trait carriers

2006 ◽  
Vol 100 (2) ◽  
pp. 427-432 ◽  
Author(s):  
Sara Fagnété ◽  
Connes Philippe ◽  
Hue Olivier ◽  
Montout-Hedreville Mona ◽  
Etienne-Julan Maryse ◽  
...  
Keyword(s):  
Oxygen Consumption ◽  
Sickle Cell ◽  
Sickle Cell Trait ◽  
Maximal Oxygen Consumption ◽  
Monocarboxylate Transporter ◽  
Transport Capacity ◽  
Transport Activity ◽  
Lactate Transport ◽  
Severe Injuries ◽  
High Concentrations

The physical and physiological behavior of sickle cell trait carriers (AS) is somewhat equivocal under strenuous conditions, although this genetic abnormality is generally considered to be a benign disorder. The occurrence of incidents and severe injuries in AS during exercise might be explained, in part, by the lactic acidosis due to a greater lactate influx into AS red blood cells (RBCs). In the present study, the RBC lactate transport activity via the different pathways was compared between AS and individuals with normal hemoglobin (AA). Sixteen Caribbean students, nine AS and seven AA, performed a progressive and maximal exercise test to determine maximal oxygen consumption. Blood samples were obtained at rest to assess haematological parameters and RBC lactate transport activity. Lactate influxes [total lactate influx and monocarboxylate transporter (MCT-1)-mediated lactate influx] into erythrocytes were measured at four external [14C]-labeled lactate concentrations (1.6, 8.1, 41, and 81.1 mM). The two groups had similar maximal oxygen consumption. Total lactate influx and lactate influx via the MCT-1 pathway were significantly higher in AS compared with AA at 1.6, 41, and 81.1 mM. The maximal lactate transport capacity for MCT-1 was higher in AS than in AA. Although AS and AA had the same maximal aerobic physical fitness, the RBCs from the sickle cell trait carriers took up more lactate at low and high concentrations than the RBCs from AA individuals. The higher MCT-1 maximal lactate transport capacity found in AS suggests greater content or greater activity of MCT-1 in AS RBC membranes.

Interindividual variation in total and carrier-mediated lactate influx into red blood cells

1998 ◽  
Vol 274 (4) ◽  
pp. R1025-R1030 ◽  
Author(s):  
Leena K. Väihkönen ◽  
A. Reeta Pösö
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Venous Blood ◽  
Age Groups ◽  
Lactate Concentration ◽  
Bimodal Distribution ◽  
Monocarboxylate Transporter ◽  
Interindividual Variation ◽  
Transport Activity ◽  
Major Carrier

To study in standardbred horses interindividual variation in the influx of lactate into red blood cells, venous blood samples were collected from 89 horses from 2 wk to 9 yr of age. For 62 horses, the rate of influx was normally distributed with a mean rate of 4.09 nmol ⋅ mg protein−1 ⋅ min−1at a lactate concentration of 10 mM, and the respective value for the other 27 horses was 0.58 nmol ⋅ mg protein−1 ⋅ min−1. At 30 mM of lactate, the rates were 8.71 and 1.97 nmol ⋅ mg protein−1 ⋅ min−1, respectively. This bimodal distribution was independent of age. In horses with high transport activity, the monocarboxylate transporter (MCT) appears to be the major carrier, whereas, in those with low transport activity, no activity of the MCT could be detected. The band 3 protein may account for 18–39% of transport activity. With all age groups combined, the transport activity tended to be higher in mares than in stallions. Lactate transport into red blood cells seems thus to be an inherent property in which participation of various transporters varies interindividually.

Characterization of anion transport system in trout red blood cell

1984 ◽  
Vol 246 (3) ◽  
pp. C330-C338 ◽  
Author(s):  
L. Romano ◽  
H. Passow
Keyword(s):  
Blood Cell ◽  
Red Blood Cells ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Sodium Dodecyl ◽  
Band 3 ◽  
Anion Transport ◽  
Disulfonic Acid ◽  
Band 3 Protein ◽  
Proton Uptake

Anion transport in the trout red blood cell is mediated by a membrane protein that selectively binds dihydro-4,4'-dithiocyanostilbene-2,2'-disulfonic acid (3H2DIDS) and that forms on sodium dodecyl sulfate (SDS)-polyacrylamide gel electropherograms a band with the same diffuse structure at the same location as the band 3 protein of the mammalian red blood cells. There exists a linear relationship between binding of H2DIDS to this protein and the inhibition of anion equilibrium exchange. At maximal inhibition about 8 X 10(6) molecules/cell are bound to the protein. The kinetics of anion transport in the trout red blood cell differ from those of mammalian red blood cells. In addition to a H2DIDS-sensitive component of sulfate transport there exists a considerable H2DIDS-insensitive component with a relative magnitude that decreases with increasing temperature. At 23 degrees C, it amounts to about 25%. The temperature dependence of the H2DIDS-sensitive component is about 15 kcal/mol instead of 32 as in human red blood cells. Cl- transport increases with increasing pH. Above pH 7.4, the rate of transport becomes too fast to be measurable with either inhibitor stop or filtration technique. SO2-4 transport is nearly pH independent over the pH range 6.5 to 7.8 and the net entry of SO2-4 in exchange against intracellular Cl-, as followed in the absence of CO2, is accompanied by little if any proton uptake. Net proton uptake becomes measurable only at temperatures above 40 degrees C. Possibly at lower and more physiological temperatures, the band 3 protein in the red blood cell of the trout accomplishes part of the SO2-4 movements without cotransporting protons.

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