Targeted inhibition of YAP/TAZ alters the biological behaviors of keloid fibroblasts

2021 ◽  
Author(s):  
Na Gao ◽  
Lulu Lu ◽  
Xiaolei Ma ◽  
Zhengyi Liu ◽  
Shuxia Yang ◽  
...  
Keyword(s):  
Targeted Inhibition ◽  
Keloid Fibroblasts

Melanoma Researchers Turn to Targeted Inhibition

2008 ◽  
Vol 39 (5) ◽  
pp. 24
Author(s):  
BRUCE JANCIN
Keyword(s):  
Targeted Inhibition

LINC01116 regulates proliferation, migration, and apoptosis of keloid fibroblasts by the TGF-β1/SMAD3 signaling via targeting miR-3141

2021 ◽  
pp. 114249
Author(s):  
Dan Wu ◽  
JinJie Zhou ◽  
Ming Tan ◽  
Yanshijing Zhou
Keyword(s):  
Tgf Β1 ◽  
Keloid Fibroblasts

scholarly journals Targeted inhibition of FGF19/FGFR cascade improves antitumor immunity and response rate in hepatocellular carcinoma

2021 ◽  
Author(s):  
David Wai Meng Tai ◽  
Thi Bich Uyen Le ◽  
Aldo Prawira ◽  
Rebecca Zhi Wen Ho ◽  
Hung Huynh
Keyword(s):  
Hepatocellular Carcinoma ◽  
Antitumor Immunity ◽  
Response Rate ◽  
Targeted Inhibition

scholarly journals Single-cell RNA-seq reveals fibroblast heterogeneity and increased mesenchymal fibroblasts in human fibrotic skin diseases

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Cheng-Cheng Deng ◽  
Yong-Fei Hu ◽  
Ding-Heng Zhu ◽  
Qing Cheng ◽  
Jing-Jing Gu ◽  
...  
Keyword(s):  
Single Cell ◽  
Skin Disease ◽  
Skin Diseases ◽  
Rna Seq ◽  
Fibrotic Disease ◽  
Functional Studies ◽  
Fibrotic Diseases ◽  
Fibroblast Heterogeneity ◽  
Keloid Fibroblasts ◽  
Excessive Accumulation

AbstractFibrotic skin disease represents a major global healthcare burden, characterized by fibroblast hyperproliferation and excessive accumulation of extracellular matrix. Fibroblasts are found to be heterogeneous in multiple fibrotic diseases, but fibroblast heterogeneity in fibrotic skin diseases is not well characterized. In this study, we explore fibroblast heterogeneity in keloid, a paradigm of fibrotic skin diseases, by using single-cell RNA-seq. Our results indicate that keloid fibroblasts can be divided into 4 subpopulations: secretory-papillary, secretory-reticular, mesenchymal and pro-inflammatory. Interestingly, the percentage of mesenchymal fibroblast subpopulation is significantly increased in keloid compared to normal scar. Functional studies indicate that mesenchymal fibroblasts are crucial for collagen overexpression in keloid. Increased mesenchymal fibroblast subpopulation is also found in another fibrotic skin disease, scleroderma, suggesting this is a broad mechanism for skin fibrosis. These findings will help us better understand skin fibrotic pathogenesis, and provide potential targets for fibrotic disease therapies.

scholarly journals Targeted inhibition of GRP78 by HA15 promotes apoptosis of lung cancer cells accompanied by ER stress and autophagy

Biology Open ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. bio053298
Author(s):  
Jingjing Wu ◽  
Youqile Wu ◽  
Xuemei Lian
Keyword(s):  
Lung Cancer ◽  
Protein Expression ◽  
Cell Viability ◽  
Er Stress ◽  
Cancer Cells ◽  
Lung Tumor ◽  
Lung Cancer Cells ◽  
Dependent Manner ◽  
Mice Model ◽  
Targeted Inhibition

ABSTRACTThis study investigated the pathophysiological role of GRP78 in the survival of lung cancer cells. Lung cancer patient data from public databases were used to analyze the expression of GRP78 and its influence on prognoses. In vivo, GRP78 protein expression was analyzed in an established urethane-induced lung tumor mouse model. In vitro, the effects of targeted inhibition of GRP78 by HA15 in lung cancer cells were assessed, with cell viability analyzed using a CCK-8 assay, cell proliferation using an EdU assay, apoptosis and cell cycle using flow cytometry, subcellular structure using electron microscopy, and relative mRNA and protein expression using RT-PCR, western blotting or immunofluorescence assays. The results showed that GRP78 was highly expressed in the lung tissue of lung cancer mice model or patients, and was associated with a poor prognosis. After inhibition of GRP78 in lung cancer cells by HA15, cell viability was decreased in a dose- and time-dependent manner, proliferation was suppressed and apoptosis promoted. Unfolded protein response signaling pathway proteins were activated, and the autophagy-related proteins and mRNAs were upregulated. Therefore, targeted inhibition of GRP78 by HA15 promotes apoptosis of lung cancer cells accompanied by ER stress and autophagy.

Ultrastructural Characteristics of Keloid Fibroblasts

1988 ◽  
Vol 10 (6) ◽  
pp. 505-508 ◽  
Author(s):  
Lois Y. Matsuoka ◽  
Jouni Uitto ◽  
Jacobo Wortsman ◽  
R. Patrick Abergel ◽  
John Dietrich
Keyword(s):  
Ultrastructural Characteristics ◽  
Keloid Fibroblasts

Deep and Superficial Keloid Fibroblasts Contribute Differentially to Tissue Phenotype in a Novel In Vivo Model of Keloid Scar

2012 ◽  
Vol 129 (6) ◽  
pp. 1259-1271 ◽  
Author(s):  
Dorothy M. Supp ◽  
Jennifer M. Hahn ◽  
Kathryn Glaser ◽  
Kevin L. McFarland ◽  
Steven T. Boyce
Keyword(s):  
In Vivo Model ◽  
Keloid Scar ◽  
Keloid Fibroblasts

scholarly journals Synergistic targeted inhibition of MEK and dual PI 3K/ mTOR diminishes viability and inhibits tumor growth of canine melanoma underscoring its utility as a preclinical model for human mucosal melanoma

2016 ◽  
Vol 29 (6) ◽  
pp. 643-655 ◽  
Author(s):  
Bih‐Rong Wei ◽  
Helen T. Michael ◽  
Charles H.C. Halsey ◽  
Cody J. Peer ◽  
Amit Adhikari ◽  
...  
Keyword(s):  
Tumor Growth ◽  
Mucosal Melanoma ◽  
Preclinical Model ◽  
Targeted Inhibition ◽  
Canine Melanoma
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