Validation of the Sysmex CS5100 coagulation analyzer and comparison to the Stago STA-R analyzer for routine coagulation parameters

Background: polytrauma is one of the emergency surgeries with a high mortality rate. One of the leading causes of death is coagulopathy that is not detected early and treated promptly. Thromboelastometry (ROTEM) is a whole blood assay that evaluates the viscoelastic properties during clot formation and clot lysis. This method can detect coagulopathy rapidly and accurately, thereby improving the management of bleeding after trauma. Objectives: describing and evaluating the correlation between ROTEM parameters and routine coagulation tests in polytrauma patients at admission. Method: 110 patients admitted to the Emergency Department, Viet Duc University Hospital from May 2021 to July 2021 were diagnosed with polytrauma. All patients underwent routine coagulation testing and ROTEM parameters at admission. Result: the average age of patients is 41.4±14.7 years old, men accounts for 77.3%, average ISS score is 24.5±6.3. The proportion of the polytrauma patients with coagulopathy by routine coagulation testing was 50.9%. A significant correlation was found between routine coagulation parameters and ROTEM: between APTT with CFT-INTEM (r=0.65; p<0.01); between PT and CFT-EXTEM (r=0.64; p<0.01); between platelet count and MCF-INTEM (r=0.56; p=0.00) and MCF-EXTEM (r=0.57; p=0.00); between fibrinogen level and MCF-INTEM (r=0.71; p=0.00), MCF-EXTEM (r=0.71; p=0.00), and MCF-FIBTEM (r=0.91; p=0.00). Conclusion: proportion of the polytrauma patients with coagulopathy by routine coagulation testing was 50.9%. A significant correlation was found between routine coagulation parameters and ROTEM

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Assessment of light transmission aggregometry on the routine coagulation analyzer Sysmex CS-2500 using CE-marked agonists from Hyphen Biomed

Platelets ◽

10.1080/09537104.2018.1528346 ◽

2018 ◽

Vol 30 (4) ◽

pp. 540-542 ◽

Cited By ~ 2

Author(s):

V.-E. Bret ◽

B. Pougault ◽

A. Guy ◽

S. Castet ◽

Y. Huguenin ◽

...

Keyword(s):

Light Transmission ◽

Light Transmission Aggregometry ◽

Coagulation Analyzer ◽

Routine Coagulation

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Fibrinogen Level Deteriorates before Other Routine Coagulation Parameters and Massive Transfusion in the Early Phase of Severe Trauma: A Retrospective Observational Study

Seminars in Thrombosis and Hemostasis ◽

10.1055/s-0034-1398379 ◽

2015 ◽

Vol 41 (01) ◽

pp. 035-042 ◽

Cited By ~ 30

Author(s):

Mineji Hayakawa ◽

Satoshi Gando ◽

Yuichi Ono ◽

Takeshi Wada ◽

Yuichiro Yanagida ◽

...

Keyword(s):

Platelet Count ◽

Early Phase ◽

Injury Severity ◽

Massive Transfusion ◽

Severe Trauma ◽

Massive Bleeding ◽

Trauma Patients ◽

Critical Levels ◽

Coagulation Parameters ◽

Routine Coagulation

In trauma, hemostatic functions should be maintained appropriately to prevent massive bleeding. This study elucidated the time-dependent changes in platelet count and coagulation variables, and the effects of disseminated intravascular coagulation (DIC) on these changes during the early phase of trauma. Trauma patients with an injury severity score ≥16 were enrolled. The critical levels of platelet count and coagulation variables were defined according to recent trauma guidelines. Massive transfusion was defined as >10 units red cell concentrate. The time from arrival at the emergency department to reaching the critical levels and meeting the criteria for massive transfusion were evaluated. Eighty trauma patients were enrolled; 35 were diagnosed with DIC on arrival. Among all patients, fibrinogen levels reached the critical level earliest among routine coagulation parameters; other routine coagulation parameters deteriorated after the patients met the criteria for massive transfusion. Routine coagulation parameters reached their critical levels earlier in DIC patients than patients without DIC. Massive transfusion was performed more frequently in DIC patients, who met the criteria earlier. During the early phase of trauma, fibrinogen levels deteriorate earlier than other routine coagulation parameters, especially in DIC patients.

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Rivaroxaban differentially influences ex vivo global coagulation assays based on the administration time

Thrombosis and Haemostasis ◽

10.1160/th10-10-0667 ◽

2011 ◽

Vol 106 (07) ◽

pp. 156-164 ◽

Cited By ~ 66

Author(s):

Christian Hesse ◽

Stratmann Gertrud ◽

Edelgard Lindhoff-Last ◽

Helen Mani

Keyword(s):

Drug Administration ◽

Ex Vivo ◽

Factor Xa ◽

Thrombin Time ◽

Thromboembolism Prophylaxis ◽

Coagulation Parameters ◽

Time Points ◽

Coagulation Assays ◽

D 12 ◽

Routine Coagulation

SummaryIt was the objective of this study to quantify the effects of rivaroxaban administration on global coagulation parameters associated with routine clinical procedures, we collected plasma samples from patients undergoing major orthopaedic surgery receiving rivaroxaban at various time points after drug administration. Forty-seven patients received rivaroxaban (10 mg daily) for venous thromboembolism prophylaxis. Blood samples were collected at four different time points: A) before surgery; B) before drug administration at day 4–5 after surgery (steady state of rivaroxaban); C) 2 hours (h) after drug administration and D) 12 h after drug administration. The prothrombin time (PT), activated partial thromboplastin time (aPTT), thrombin time (TT), antithrombin (AT) level, fibrinogen level by Clauss method (FibC), and derived fibrinogen (dFIB) level were assessed with various reagents. At 2 h after rivaroxaban administration, the PT and aPTT clotting times were significantly prolonged to different extents up to 1.4 fold, whereas 12 h after drug administration, no significant effect was observed. Rivaroxaban administration had no influence on the TT or the FibC concentration. The dFIB assay was differentially affected by rivaroxaban when different reagents were tested. The AT assay dependent on thrombin activity was not influenced by rivaroxaban, whereas the AT levels dependent on factor Xa activity were significantly increased by rivaroxaban. Clinicians should be aware of the time-dependent influence of rivaroxaban on factor Xa-dependent routine coagulation assays. Therefore, routine coagulation parameters should be assessed directly before drug administration to keep the interaction of rivaroxaban low.

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Association Between the Change of Coagulation Parameters and Clinical Prognosis in Acute Ischemic Stroke Patients After Intravenous Thrombolysis With rt-PA

Clinical and Applied Thrombosis/Hemostasis ◽

10.1177/10760296211039285 ◽

2021 ◽

Vol 27 ◽

pp. 107602962110392

Author(s):

Yu Wang ◽

Jia Zhang ◽

Zhentang Cao ◽

Qian Zhang ◽

Xingquan Zhao

Keyword(s):

Ischemic Stroke ◽

Acute Stroke ◽

Acute Ischemic Stroke ◽

Thrombin Time ◽

Stroke Patients ◽

Short Term ◽

Distribution Width ◽

Clinical Prognosis ◽

Coagulation Parameters ◽

Routine Coagulation

Acute ischemic stroke patients with intravenous (IV) recombinant tissue plasminogen activator (rt-PA) thrombolysis have different outcomes. The degree of thrombolysis depends largely on the delicate balance of coagulation and fibrinolysis. Thus, our study aimed to investigate the prognostic value of routine coagulation parameters in acute stroke patients treated with rt-PA. From December 2016 to October 2018, consecutive patients treated with standard-dose IV rt-PA within 4.5 h of stroke onset were collected in Beijing Tiantan Hospital. Routine coagulation parameters, including platelet count, mean platelet volume, platelet distribution width, prothrombin time (PT), activated partial thromboplastin time, thrombin time, and fibrinogen, were measured at baseline (h0) and 24 h (h24) after thrombolysis. The change of coagulation parameters was defined as the (h24-h0)/h0 ratio. The prognosis included short-term outcome at 24 h and functional outcome at 3 months. A total of 267 patients were investigated (188 men and 79 women) with a mean age of 60.88 ± 12.31 years. In total, 9 patients had early neurological deterioration within 24 h, and 99 patients had an unfavorable outcome at the 3-month visit. In multivariate logistic regression, the (h24-h0)/h0 of PT was associated with unfavorable functional outcomes at 3 months (odds ratio: 1.42, 95% confidence interval: 1.02-2.28). While the change of other coagulation parameters failed to show any correlation with short-term or long-term prognosis. In conclusion, the prolongation of PT from baseline to 24 h after IV rt-PA increases the risk of 3-month unfavorable outcomes in acute stroke patients.

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Performance Evaluation of the CS-5100 Coagulation Analyzer for Special Coagulation Parameters

Laboratory Medicine Online ◽

10.3343/lmo.2020.10.1.25 ◽

2020 ◽

Vol 10 (1) ◽

pp. 25

Author(s):

Bohyun Kim ◽

Seongsoo Jang ◽

Yu-Jin Lee ◽

Nuree Park ◽

Young-Uk Cho ◽

...

Keyword(s):

Performance Evaluation ◽

Coagulation Parameters ◽

Coagulation Analyzer

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Assessment of platelet function on the routine coagulation analyzer Sysmex CS-2000i

Platelets ◽

10.1080/09537104.2017.1353683 ◽

2017 ◽

Vol 29 (1) ◽

pp. 95-97 ◽

Cited By ~ 8

Author(s):

Corinne Frère ◽

Katsushi Kobayashi ◽

Claire Dunois ◽

Jean Amiral ◽

Pierre-Emmanuel Morange ◽

...

Keyword(s):

Platelet Function ◽

Coagulation Analyzer ◽

Routine Coagulation

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Use of an Automated Coagulation Analyzer to Perform Heparin Neutralization With Polybrene in Blood Samples for Routine Coagulation Testing: Practical, Rapid, and Inexpensive

Archives of Pathology & Laboratory Medicine ◽

10.5858/arpa.2012-0599-oa ◽

2013 ◽

Vol 137 (11) ◽

pp. 1641-1647 ◽

Cited By ~ 4

Author(s):

Panutsaya Tientadakul ◽

Chulalak Kongkan ◽

Wimol Chinswangwatanakul

Keyword(s):

Prothrombin Time ◽

Blood Samples ◽

Coagulation Testing ◽

Coagulation Tests ◽

Clinically Significant ◽

Aptt Reagent ◽

Heparin Neutralization ◽

Coagulation Analyzer ◽

Suitable Sequence ◽

Routine Coagulation

Context.—Heparin contamination in blood samples may cause false prolongation of activated partial thromboplastin time (aPTT) and prothrombin time results. Polybrene can neutralize heparin, but it affects coagulation by itself. Objectives.——To determine the optimal concentration of polybrene to neutralize heparin, to determine the suitable sequence of reagents for the neutralization method performed on the analyzer at the same time as prothrombin time and aPTT testing, and to detect the heparin contamination in blood samples for coagulation tests in our hospital using this method. Design.—Various concentrations of heparin were added to 10 normal and 76 abnormal plasma samples to study the efficacy of polybrene. Two programs of reagent sequencing for aPTT with polybrene performed on the analyzer were tested. Samples suspected of heparin contamination according to our criteria were selected for neutralization during a 3-month period. Results.——The optimal final concentration of polybrene was 25 μg/mL. Polybrene should be added after the aPTT reagent to minimize its interference effect. Even though results of prothrombin time and aPTT after neutralization did not equal those before the spike of heparin, the differences might not be clinically significant. Eighty-one of 4921 samples (1.6%) were selected for aPTT with the neutralization method, and the detection rate of heparin contamination was 84% (68 of 81), giving an overall incidence of 1.4% (68 of 4921). Conclusions.—This method is inexpensive and can be performed rapidly with prothrombin time and aPTT on the automated analyzer, which makes it easy to practice with no need for extra plasma volumes.

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