scholarly journals Differential Regulation by IL-1β and EGF of Expression of Three Different Hyaluronan Synthases in Oral Mucosal Epithelial Cells and Fibroblasts and Dermal Fibroblasts: Quantitative Analysis Using Real-Time RT-PCR

2004 ◽  
Vol 122 (3) ◽  
pp. 631-639 ◽  
Author(s):  
Yoichi Yamada ◽  
Naoki Itano ◽  
Ken-ichiro Hata ◽  
Minoru Ueda ◽  
Koji Kimata
Keyword(s):  
Quantitative Analysis ◽  
Epithelial Cells ◽  
Real Time ◽  
Differential Regulation ◽  
Dermal Fibroblasts ◽  
Rt Pcr ◽  
Hyaluronan Synthases

scholarly journals Quantitative Analysis of Multidrug-resistancemdr1Gene Expression in Head and Neck Cancer by Real-time RT-PCR

2002 ◽  
Vol 93 (11) ◽  
pp. 1230-1236 ◽  
Author(s):  
Ching-Ping Tseng ◽  
Ann-Joy Cheng ◽  
Joseph Tung-Chieh Chang ◽  
Chin-Hsiao Tseng ◽  
Hung-Ming Wang ◽  
...  
Keyword(s):  
Head And Neck Cancer ◽  
Quantitative Analysis ◽  
Head And Neck ◽  
Real Time ◽  
Neck Cancer ◽  
Rt Pcr

scholarly journals Quantitative Analysis of the Relative Transcript Levels of ABC Transporter Atr Genes in Aspergillus nidulans by Real-Time Reverse Transcription-PCR Assay

2002 ◽  
Vol 68 (3) ◽  
pp. 1351-1357 ◽  
Author(s):  
Camile Pizeta Semighini ◽  
Mozart Marins ◽  
Maria Helena S. Goldman ◽  
Gustavo Henrique Goldman
Keyword(s):  
Quantitative Analysis ◽  
Real Time ◽  
Aspergillus Nidulans ◽  
Abc Transporter ◽  
Reverse Transcription ◽  
Wild Type ◽  
Rt Pcr ◽  
Transcript Levels ◽  
Reverse Transcription Pcr ◽  
Nitroquinoline Oxide

ABSTRACT The development of assays for quantitative analysis of the relative transcript levels of ABC transporter genes by real-time reverse transcription-PCR (RT-PCR) might provide important information about multidrug resistance in filamentous fungi. Here, we evaluate the potential of real-time RT-PCR to quantify the relative transcript levels of ABC transporter Atr genes from Aspergillus nidulans. The AtrA to AtrD genes showed different and higher levels in the presence of structurally unrelated drugs, such as camptothecin, imazalil, itraconazole, hygromycin, and 4-nitroquinoline oxide. We also verified the relative transcript levels of the Atr genes in the A. nidulans imazalil-resistant mutants. These genes displayed a very complex pattern in different ima genetic backgrounds. The imaB mutant has higher basal transcript levels of AtrB and -D than those of the wild-type strain. The levels of these two genes are comparable when the imaB mutant is grown in the presence and absence of imazalil. The imaC, -D, and -H mutants have higher basal levels of AtrA than that of the wild type. The same behavior is observed for the relative transcript levels of AtrB in the imaG mutant background.

Real-time RT-PCR for the detection and quantitative analysis of equine rhinitis viruses

2010 ◽  
Vol 42 (2) ◽  
pp. 98-104 ◽  
Author(s):  
M. QUINLIVAN ◽  
G. MAXWELL ◽  
P. LYONS ◽  
S. ARKINS ◽  
A. CULLINANE
Keyword(s):  
Quantitative Analysis ◽  
Real Time ◽  
Rt Pcr

Quantitative analysis of PPARδ mRNA by real-time RT-PCR in 86 rectal cancer tissues

2006 ◽  
Vol 32 (2) ◽  
pp. 181-185 ◽  
Author(s):  
L. Yang ◽  
Z.-G. Zhou ◽  
H.-Z. Luo ◽  
B. Zhou ◽  
Q.-J. Xia ◽  
...  
Keyword(s):  
Rectal Cancer ◽  
Quantitative Analysis ◽  
Real Time ◽  
Rt Pcr ◽  
Cancer Tissues
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