scholarly journals Implications of Using the ND1 Gene as a Control Region for Real-Time PCR Analysis of Mitochondrial DNA Deletions in Human Skin

2004 ◽  
Vol 122 (6) ◽  
pp. 1518-1521 ◽  
Author(s):  
Andrew Harbottle ◽  
Kim J. Krishnan ◽  
Mark A. Birch-Machin
Keyword(s):  
Mitochondrial Dna ◽  
Real Time ◽  
Human Skin ◽  
Control Region ◽  
Real Time Pcr ◽  
Pcr Analysis ◽  
Dna Deletions

scholarly journals Triplex real-time PCR–an improved method to detect a wide spectrum of mitochondrial DNA deletions in single cells

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Karolina A. Rygiel ◽  
John P. Grady ◽  
Robert W. Taylor ◽  
Helen A. L. Tuppen ◽  
Doug M. Turnbull
Keyword(s):  
Mitochondrial Dna ◽  
Real Time ◽  
Real Time Pcr ◽  
Wide Spectrum ◽  
Single Cells ◽  
Improved Method ◽  
Dna Deletions

Application of mitochondrial DNA analysis for microbial source tracking purposes in shellfish harvesting waters

2010 ◽  
Vol 61 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Craig Baker-Austin ◽  
Rachel Rangdale ◽  
James Lowther ◽  
David N. Lees
Keyword(s):  
Mitochondrial Dna ◽  
Real Time ◽  
Real Time Pcr ◽  
Dna Analysis ◽  
Pcr Amplification ◽  
Source Tracking ◽  
Pcr Analysis ◽  
Taqman Probes ◽  
Faecal Matter ◽  
Species Specific

We present a method for the reliable detection and source characterisation of faecal pollution in water and shellfish matrices, utilising real-time PCR analysis of mitochondrial DNA targets. In this study we designed real-time PCR (TaqMan) probes to target human, bovine, ovine and swine mtDNA. PCR amplification using species-specific TaqMan probes on faecal matter and mixed effluent slurries revealed no cross-reactions between species of interest and other vertebrate faecal matter. Performed as a single blind experiment we were able to correctly identify faecal material in 17/20 effluents (85% correct). mtDNA degrades relatively quickly in faecally-spiked water samples (∼2 weeks), a similar timeframe of environmental persistence to several bacterial faecal indictors, highlighting its applicability. The procedure described here is specific, rapid (<5 hours) and sensitive. These results confirm the suitability of using species-specific mtDNA as an indicator in source tracking studies in surface waters, shellfish harvesting areas and shellfish matrices.

A multiplex real-time PCR method to detect and quantify mitochondrial DNA deletions in individual cells

2007 ◽  
Vol 370 (1) ◽  
pp. 127-129 ◽  
Author(s):  
Kim J. Krishnan ◽  
Andreas Bender ◽  
Robert W. Taylor ◽  
Douglass M. Turnbull
Keyword(s):  
Mitochondrial Dna ◽  
Real Time ◽  
Real Time Pcr ◽  
Dna Deletions ◽  
Pcr Method
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