Real-Time PCR Analysis of Peripheral Blood: A Non-Invasive Technology That Can Be Used to Assess Tumor Burden in Breast Cancer Patients

Purpose of StudyOver 240,000 individuals are diagnosed with breast cancer (BrCa) of which 12,000 individuals carry BRCA germline mutations. MicroRNA dysregulation is common in malignancy and may correlate with germline mutations.Aims:1. Analyze microRNAs in patients with breast cancer with or without BRCA germ line mutations, with and without cancer.2. Identify molecular BRCA mutant patients to deduct reasons for accelerated malignancy.Methods UsedWe analyzed plasma miR expression from 94 br cancer patients (41 BRCA positive) relative to 24 normal controls. All samples were collected between 2010 and 2014 and survival data was known for all cancer patients. TaqMan Open Array panel was used to simultaneously run hundreds of microRNA assays in the Applied Biosystem Open array real time PCR. Using AB open array real time PCR, 756 miRNA species were detected. Two-sample t-test was used for all 2-sample comparison and ANOVA followed by Tukey HSD post-hoc test to compare the miRs mean differences. All tests were 2-tailed and results with a p<0.05 were considered statistically significant.Summary of ResultsBRCA+underexpressed hsa-mir-10a and hsa-mir-376c and over-expressed Hsa- mir- 326 and Hsa-mir-143 relative to BRCA-; p<0.05.Using Coremine data mining linking genes and diseases differentially expressed circulating miRs are linked to tumor suppressor TGFbeta/SMAD3.ConclusionsThe early onset of breast cancer in BRCA mutant patients may recapitulate the pro-oncogenic effects of TGF-β. The context dependent SMAD3 binding & tumor suppression TGF-β effects are abrogated in BRCA mutant patients. TGF-β/Smad3 tumor-suppressor signature suppresses local inflammation in the tumor microenvironment.

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HER-2/neu gene amplification assessment in breast cancer patients in Isfahan province by real time PCR, differential PCR and immunohistochemistry

Gene ◽

10.1016/j.gene.2012.01.025 ◽

2012 ◽

Vol 497 (2) ◽

pp. 237-242 ◽

Cited By ~ 6

Author(s):

Zohreh Hojati ◽

Elham Orangi

Keyword(s):

Breast Cancer ◽

Real Time ◽

Cancer Patients ◽

Gene Amplification ◽

Real Time Pcr ◽

Breast Cancer Patients ◽

Isfahan Province ◽

Her 2

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Methylomics of breast cancer: Seeking epimarkers in peripheral blood of young subjects

Tumor Biology ◽

10.1177/1010428317695040 ◽

2017 ◽

Vol 39 (3) ◽

pp. 101042831769504 ◽

Cited By ~ 8

Author(s):

Golnaz Khakpour ◽

Mehrdad Noruzinia ◽

Pantea Izadi ◽

Fatemeh Karami ◽

Mohammad Ahmadvand ◽

...

Keyword(s):

Breast Cancer ◽

Cancer Patients ◽

Peripheral Blood ◽

Blood Cells ◽

White Blood Cells ◽

Young Patients ◽

Breast Cancer Patients ◽

Tissue Samples ◽

Non Invasive ◽

Cancer Pathogenesis

Critical roles of epigenomic alterations in the pathogenesis of breast cancer have recently seized great attentions toward finding epimarkers in either non-invasive or semi-non-invasive samples as well as peripheral blood. In this way, methylated DNA immunoprecipitation microarray (MeDIP-chip) was performed on DNA samples isolated from white blood cells of 30 breast cancer patients compared to 30 healthy controls. A total of 1799 differentially methylated regions were identified including SLC6A3, Rab40C, ZNF584, and FOXD3 whose significant methylation differences were confirmed in breast cancer patients through quantitative real-time polymerase chain reaction. Hypermethylation of APC, HDAC1, and GSK1 genes has been previously reported in more than one study on tissue samples of breast cancer. Methylation of those aforementioned genes in white blood cells of our young patients not only relies on their importance in breast cancer pathogenesis but also may highlight their potential as early epimarkers that makes further assessments necessary in large cohort studies.

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