scholarly journals Real-time PCR analysis of a 3895 bp mitochondrial DNA deletion in nonmelanoma skin cancer and its use as a quantitative marker for sunlight exposure in human skin

2006 ◽  
Vol 94 (12) ◽  
pp. 1887-1893 ◽  
Author(s):  
A Harbottle ◽  
M A Birch-Machin
Keyword(s):  
Mitochondrial Dna ◽  
Skin Cancer ◽  
Real Time ◽  
Human Skin ◽  
Real Time Pcr ◽  
Nonmelanoma Skin Cancer ◽  
Sunlight Exposure ◽  
Pcr Analysis ◽  
Mitochondrial Dna Deletion ◽  
Dna Deletion

Photosensitivity

2010 ◽  
pp. 4665-4671
Author(s):  
Jane McGregor
Keyword(s):  
Skin Cancer ◽  
Human Skin ◽  
Sun Exposure ◽  
Nonmelanoma Skin Cancer ◽  
Sunlight Exposure ◽  
Normal Human Skin ◽  
Normal Individuals ◽  
Normal Human ◽  
Fair Skin

Normal human skin is photosensitive in that it reddens following acute sunlight exposure and tans and thickens following chronic sunlight exposure. Skin cancer, particularly nonmelanoma skin cancer, is also a consequence of high cumulative sun exposure in genetically predisposed normal individuals (predominantly those with fair skin)....

Application of mitochondrial DNA analysis for microbial source tracking purposes in shellfish harvesting waters

2010 ◽  
Vol 61 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Craig Baker-Austin ◽  
Rachel Rangdale ◽  
James Lowther ◽  
David N. Lees
Keyword(s):  
Mitochondrial Dna ◽  
Real Time ◽  
Real Time Pcr ◽  
Dna Analysis ◽  
Pcr Amplification ◽  
Source Tracking ◽  
Pcr Analysis ◽  
Taqman Probes ◽  
Faecal Matter ◽  
Species Specific

We present a method for the reliable detection and source characterisation of faecal pollution in water and shellfish matrices, utilising real-time PCR analysis of mitochondrial DNA targets. In this study we designed real-time PCR (TaqMan) probes to target human, bovine, ovine and swine mtDNA. PCR amplification using species-specific TaqMan probes on faecal matter and mixed effluent slurries revealed no cross-reactions between species of interest and other vertebrate faecal matter. Performed as a single blind experiment we were able to correctly identify faecal material in 17/20 effluents (85% correct). mtDNA degrades relatively quickly in faecally-spiked water samples (∼2 weeks), a similar timeframe of environmental persistence to several bacterial faecal indictors, highlighting its applicability. The procedure described here is specific, rapid (<5 hours) and sensitive. These results confirm the suitability of using species-specific mtDNA as an indicator in source tracking studies in surface waters, shellfish harvesting areas and shellfish matrices.

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