scholarly journals Transforming Growth Factor-β1 Regulates the Expression of the High-Affinity Receptor for IgE on CD34+ Stem Cell-Derived CD1a+ Dendritic Cells In Vitro

2004 ◽  
Vol 123 (4) ◽  
pp. 676-682 ◽  
Author(s):  
Jean-Pierre Allam ◽  
Elisabeth Klein ◽  
Thomas Bieber ◽  
Natalija Novak
Keyword(s):  
Dendritic Cells ◽  
Stem Cell ◽  
Growth Factor ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Β1 ◽  
High Affinity ◽  
High Affinity Receptor ◽  
Affinity Receptor

IL-4 induces the intracellular expression of the α chain of the high-affinity receptor for IgE in in vitro–generated dendritic cells

2000 ◽  
Vol 105 (1) ◽  
pp. 150-156 ◽  
Author(s):  
Elisabeth Geiger ◽  
Roland Magerstaedt ◽  
Jörg H.M. Weßendorf ◽  
Stefan Kraft ◽  
Daniel Hanau ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
High Affinity ◽  
Intracellular Expression ◽  
High Affinity Receptor ◽  
Affinity Receptor ◽  
Α Chain

scholarly journals Expression of nerve growth factor and its high-affinity receptor Trk-A in the rat pancreas during embryonic and fetal life

1998 ◽  
Vol 156 (3) ◽  
pp. 431-439 ◽  
Author(s):  
F Miralles ◽  
P Philippe ◽  
P Czernichow ◽  
R Scharfmann
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Endocrine Cells ◽  
Fetal Life ◽  
Rat Pancreas ◽  
High Affinity ◽  
High Affinity Receptor ◽  
Affinity Receptor ◽  
Trk A

The expression of functional receptors for nerve growth factor in insulin-producing cell lines grown in vitro has recently been demonstrated. The possible importance of signals transduced via these receptors in the control of islet maturation has been proposed based on data obtained using an in vitro culture system. To further support this hypothesis, we have studied the expression of Trk-A, the high-affinity receptor for NGF, in vivo during the embryonic and fetal development of the rat pancreas. We have also examined the expression of NGF during the same period. Immunohistological analysis shows that at embryonic day 11 (E11), Trk-A is expressed by the epithelial cells of the presumptive pancreas. The few pancreatic endocrine cells present at that stage express Trk-A. At E12 and E16, Trk-A expression was detected in the developing ductal network. The endocrine cells located in the ducts express Trk-A while those that have migrated into the surrounding mesenchyme now stain negative for Trk-A. By E20, Trk-A expression by ductal cells has considerably decreased and can be detected only in small ducts closely associated with islet-like structures. These islet-like structures stain negative for Trk-A. After birth, insulin-positive cells arranged into islets re-express Trk-A. During the same period, NGF mRNA is found to be expressed in the developing pancreas. The expression of Trk-A and its ligand NGF in the pancreas during embryonic and fetal life suggests that NGF and its receptor could play an important role in the development of the pancreas.

Parathyroid Hormone and Transforming Growth Factor-β1 Coregulate Chondrocyte Differentiation In Vitro

Endocrine ◽  
2000 ◽  
Vol 13 (3) ◽  
pp. 305-313 ◽  
Author(s):  
E. Nasatzky ◽  
E. Azran ◽  
D. D. Dean ◽  
Barbara D. Boyan ◽  
Z. Schwartz
Keyword(s):  
Parathyroid Hormone ◽  
Growth Factor ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Β1 ◽  
Chondrocyte Differentiation

scholarly journals Transforming Growth Factor β1 (TGF-β1) Promotes Endothelial Cell Survival during In Vitro Angiogenesis via an Autocrine Mechanism Implicating TGF-α Signaling

2001 ◽  
Vol 21 (21) ◽  
pp. 7218-7230 ◽  
Author(s):  
Francesc Viñals ◽  
Jacques Pouysségur
Keyword(s):  
Endothelial Cells ◽  
Growth Factor ◽  
Cell Survival ◽  
Network Formation ◽  
Egf Receptor ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Β1 ◽  
In Vitro Angiogenesis ◽  
Tgf Β1

ABSTRACT Mouse capillary endothelial cells (1G11 cell line) embedded in type I collagen gels undergo in vitro angiogenesis. Cells rapidly reorganize and form capillary-like structures when stimulated with serum. Transforming growth factor β1 (TGF-β1) alone can substitute for serum and induce cell survival and tubular network formation. This TGF-β1-mediated angiogenic activity depends on phosphatidylinositol 3-kinase (PI3K) and p42/p44 mitogen-activated protein kinase (MAPK) signaling. We showed that specific inhibitors of either pathway (wortmannin, LY-294002, and PD-98059) all suppressed TGF-β1-induced angiogenesis mainly by compromising cell survival. We established that TGF-β1 stimulated the expression of TGF-α mRNA and protein, the tyrosine phosphorylation of a 170-kDa membrane protein representing the epidermal growth factor (EGF) receptor, and the delayed activation of PI3K/Akt and p42/p44 MAPK. Moreover, we showed that all these TGF-β1-mediated signaling events, including tubular network formation, were suppressed by incubating TGF-β1-stimulated endothelial cells with a soluble form of an EGF receptor (ErbB-1) or tyrphostin AG1478, a specific blocker of EGF receptor tyrosine kinase. Finally, addition of TGF-α alone poorly stimulated angiogenesis; however, by reducing cell death, it strongly potentiated the action of TGF-β1. We therefore propose that TGF-β1 promotes angiogenesis at least in part via the autocrine secretion of TGF-α, a cell survival growth factor, activating PI3K/Akt and p42/p44 MAPK.

Growth factor expression pattern of homologous feeder layer for culturing buffalo embryonic stem cell-like cells

2012 ◽  
Vol 24 (8) ◽  
pp. 1098 ◽  
Author(s):  
Ruchi Sharma ◽  
Aman George ◽  
Nitin M. Kamble ◽  
Manmohan S. Chauhan ◽  
Suresh Singla ◽  
...  
Keyword(s):  
Stem Cell ◽  
Growth Factor ◽  
Transforming Growth Factor ◽  
Embryonic Stem ◽  
Transforming Growth Factor Β1 ◽  
Activin A ◽  
Feeder Layer ◽  
Es Cell ◽  
Fetal Fibroblasts ◽  
Tgf Β1

The present study examined the expression profile of buffalo fetal fibroblasts (BFF) used as a feeder layer for embryonic stem (ES) cell-like cells. The expression of important growth factors was detected in cells at different passages. Mitomycin-C inactivation increased relative expression levels of ACTIVIN-A, TGF-β1, BMP-4 and GREMLIN but not of fibroblast growth factor-2 (FGF-2). The expression level of ACTIVIN-A, transforming growth factor-β1 (TGF-β1), bone morphogenetic protein-4 (BMP-4) and FGF-2 was similar in buffalo fetal fibroblast (BFF) cultured in stem cell medium (SCM), SCM + 1000 IU mL–1 leukemia inhibitory factor (LIF), SCM + 5 ng mL–1 FGF-2 or SCM + LIF + FGF-2 for 24 h whereas GREMLIN expression was higher in FGF-2-supplemented groups. In spent medium, the concentration of ACTIVIN-A was higher in FGF-2-supplemented groups whereas that of TGF-β1 was similar in SCM and LIF + FGF-2, which was higher than when either LIF or FGF-2 was used alone. Following culture of ES cell-like cells on a feeder layer for 24 h, the TGF-β1 concentration was higher with LIF+FGF-2 than with LIF or FGF-2 alone which, in turn, was higher than that in SCM. In the LIF + FGF-2 group, the concentration of TGF-β1 was lower and that of ACTIVIN-A was higher in spent medium at 24 h than at 48 h of culture. These results suggest that BFF produce signalling molecules that may help in self-renewal of buffalo ES cell-like cells.

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