An assay for parvovirus B19 neutralizing antibodies based on human hepatocarcinoma cell lines

Transfusion ◽  
2004 ◽  
Vol 44 (9) ◽  
pp. 1340-1343 ◽  
Author(s):  
Perrine Caillet-Fauquet ◽  
Mario Di Giambattista ◽  
Marie-Louise Draps ◽  
Vincent Hougardy ◽  
Yvan De Launoit ◽  
...  
Keyword(s):  
Cell Lines ◽  
Neutralizing Antibodies ◽  
Parvovirus B19 ◽  
Hepatocarcinoma Cell

scholarly journals Yunnan orbivirus, a new orbivirus species isolated from Culex tritaeniorhynchus mosquitoes in China

2005 ◽  
Vol 86 (12) ◽  
pp. 3409-3417 ◽  
Author(s):  
Houssam Attoui ◽  
Fauziah Mohd Jaafar ◽  
Mourad Belhouchet ◽  
Nicolas Aldrovandi ◽  
Sanju Tao ◽  
...  
Keyword(s):  
Cell Lines ◽  
Neutralizing Antibodies ◽  
Yunnan Province ◽  
Coat Proteins ◽  
Outer Coat ◽  
Culex Tritaeniorhynchus ◽  
Specific Pcr ◽  
Mammalian Cell Lines ◽  
Mosquito Cell Lines ◽  
A New Species

An orbivirus designated Yunnan orbivirus (YUOV) was isolated from Culex tritaeniorhynchus mosquitoes collected in the Yunnan province of China. Electron microscopy showed particles with typical orbivirus morphology. The YUOV genome was sequenced completely and compared with previously characterized orbivirus genomes. Significant identity scores were detected between proteins encoded by the segments (Seg-1 to Seg-10) of YUOV and those encoded by their homologues in insect-borne and tick-borne orbiviruses. Analysis of VP1 (Pol) and VP2 (T2, which correlates with the virus serogroup) indicated that YUOV is a new species of the genus Orbivirus that is unrelated to the other insect-borne orbiviruses. The replication of YUOV in mosquito cell lines was restricted to Aedes albopictus cells and the virus failed to replicate in mammalian cell lines. However, intraperitoneal injection of virus into naïve mice resulted in productive, non-lethal virus replication and viraemia. Infected mice developed serum neutralizing antibodies and were protected against a new infection challenge. Sequence analysis of clones from the segments encoding outer coat proteins (Seg-3 and Seg-6) of YUOV recovered from mouse blood did not show significant changes in the sequences. The availability of the complete genome sequence will facilitate the development of sequence-specific PCR assays for the study of YUOV epidemiology in the field.

scholarly journals Antiestrogen-resistant human breast cancer cells require activated Protein Kinase B/Akt for growth

2005 ◽  
Vol 12 (3) ◽  
pp. 599-614 ◽  
Author(s):  
T Frogne ◽  
J S Jepsen ◽  
S S Larsen ◽  
C K Fog ◽  
B L Brockdorff ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Patients ◽  
Cell Lines ◽  
Human Breast Cancer ◽  
Neutralizing Antibodies ◽  
Breast Cancer Cells ◽  
Resistant Cell ◽  
Breast Cancer Patients ◽  
Human Breast ◽  
Mcf 7

Development of acquired resistance to antiestrogens is a major clinical problem in endocrine treatment of breast cancer patients. The IGF system plays a profound role in many cancer types, including breast cancer. Thus, overexpression and/or constitutive activation of the IGF-I receptor (IGF-IR) or different components of the IGF-IR signaling pathway have been reported to render breast cancer cells less estrogen dependent and capable of sustaining cell proliferation in the presence of antiestrogens. In this study, growth of the antiestrogen-sensitive human breast cancer cell line MCF-7 was inhibited by treatment with IGF-IR-neutralizing antibodies. In contrast, IGF-IR-neutralizing antibodies had no effect on growth of two different antiestrogen-resistant MCF-7 sublines. A panel of antiestrogen-resistant cell lines was investigated for expression of IGF-IR and either undetectable or severely reduced IGF-IR levels were observed. No increase in insulin receptor substrate 1 (IRS-1) or total PKB/Akt (Akt) was detected in the resistant cell lines. However, a significant increase in phosphorylated Akt (pAkt) was found in four of six antiestrogen-resistant cell lines. Overexpression of pAkt was associated with increased Akt kinase activity in both a tamoxifen- and an ICI 182,780-resistant cell line. Inhibition of Akt phosphorylation by the phosphatidylinositol 3-kinase (PI3-K) inhibitor wortmannin or the Akt inhibitor SH-6 (structurally modified phosphatidyl inositol ether liquid analog PIA 6) resulted in a more pronounced growth inhibitory effect on the antiestrogen-resistant cells compared with the parental cells, suggesting that signaling via Akt is required for antiestrogen-resistant cell growth in at least a subset of our antiestrogen-resistant cell lines. PTEN expression and activity was not decreased in cell lines overexpressing pAkt. Our data demonstrate that Akt is a target for treatment of antiestrogen-resistant breast cancer cell lines and we suggest that antiestrogen-resistant breast cancer patients may benefit from treatment targeted to inhibit Akt signaling.

Divergent expression and roles for caveolin-1 in mouse hepatocarcinoma cell lines with varying invasive ability

2006 ◽  
Vol 345 (1) ◽  
pp. 486-494 ◽  
Author(s):  
Huimin Zhou ◽  
Li Jia ◽  
Shujing Wang ◽  
Hongmei Wang ◽  
Haiying Chu ◽  
...  
Keyword(s):  
Cell Lines ◽  
Invasive Ability ◽  
Caveolin 1 ◽  
Hepatocarcinoma Cell

scholarly journals Human parvovirus B19 infection in HIV-positive patients

2001 ◽  
Vol 34 (3) ◽  
pp. 239-242 ◽  
Author(s):  
Fábio S. Aguiar ◽  
Daniella P. Lopes ◽  
Anna Ricordi Bazin ◽  
Sérgio Setúbal ◽  
Bernard J. Cohen ◽  
...  
Keyword(s):  
Neutralizing Antibodies ◽  
Parvovirus B19 ◽  
Blot Hybridization ◽  
Epidemiological Studies ◽  
Hiv Positive ◽  
Dot Blot ◽  
Cd4 Cell Count ◽  
High Prevalence ◽  
Parvovirus B19 Infection ◽  
Cd4 Cell

Parvovirus B19 infects predominantly erythroid cells, leading to transient inhibition of erythropoiesis. Immunocompromised patients may be unable to produce neutralizing antibodies and may develop severe chronic anemia. Epidemiological studies done on Niterói population showed that B19 infection occurs periodically in late spring and summer. We report a study from 55 HIV infected patients attending an infectious diseases outpatient clinic in this city during a 5-month period in which B19 circulation was well documented. All patients were under anti-retroviral therapy. No anti-B19 IgM was found, but a high prevalence of IgG anti-B19 (91%) was observed. In six patients, B19 DNA was found by dot-blot hybridization techniques, but this was not confirmed by PCR. None of these 6 patients manifested anemia and only one had CD4 cell count below 200 x 10(7)/L. We conclude that persistent infection causing anemia is an infrequent finding in our HIV positive patients under drug therapy.

scholarly journals Inhibition of HSF1 suppresses the growth of hepatocarcinoma cell lines in vitro and AKT-driven hepatocarcinogenesis in mice

Oncotarget ◽  
2017 ◽  
Vol 8 (33) ◽  
pp. 54149-54159 ◽  
Author(s):  
Antonio Cigliano ◽  
Chunmei Wang ◽  
Maria G. Pilo ◽  
Marta Szydlowska ◽  
Stefania Brozzetti ◽  
...  
Keyword(s):  
Cell Lines ◽  
Hepatocarcinoma Cell

scholarly journals Salirasib inhibits the growth of hepatocarcinoma cell lines in vitro and tumor growth in vivo through ras and mTOR inhibition

2010 ◽  
Vol 9 (1) ◽  
pp. 256 ◽  
Author(s):  
Nicolas Charette ◽  
Christine De Saeger ◽  
Valérie Lannoy ◽  
Yves Horsmans ◽  
Isabelle Leclercq ◽  
...  
Keyword(s):  
Tumor Growth ◽  
Cell Lines ◽  
Mtor Inhibition ◽  
Hepatocarcinoma Cell

scholarly journals Ex Vivo-Generated CD36+ Erythroid Progenitors Are Highly Permissive to Human Parvovirus B19 Replication

2007 ◽  
Vol 82 (5) ◽  
pp. 2470-2476 ◽  
Author(s):  
Susan Wong ◽  
Ning Zhi ◽  
Claudia Filippone ◽  
Keyvan Keyvanfar ◽  
Sachiko Kajigaya ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Real Time ◽  
Cell Lines ◽  
Ex Vivo ◽  
Parvovirus B19 ◽  
Erythroid Progenitor ◽  
Hematopoietic Stem ◽  
Human Virus ◽  
Erythroid Progenitor Cells ◽  
Large Numbers

ABSTRACT The pathogenic parvovirus B19 (B19V) has an extreme tropism for human erythroid progenitor cells. In vitro, only a few erythroid leukemic cell lines (JK-1 and KU812Ep6) or megakaryoblastoid cell lines (UT7/Epo and UT7/Epo-S1) with erythroid characteristics support B19V replication, but these cells are only semipermissive. By using recent advances in generating large numbers of human erythroid progenitor cells (EPCs) ex vivo from hematopoietic stem cells (HSCs), we produced a pure population of CD36+ EPCs expanded and differentiated from CD34+ HSCs and assessed the CD36+ EPCs for their permissiveness to B19V infection. Over more than 3 weeks, cells grown in serum-free medium expanded more than 800,000-fold, and 87 to 96% of the CD36+ EPCs were positive for globoside, the cellular receptor for B19V. Immunofluorescence (IF) staining showed that about 77% of the CD36+ EPCs were positive for B19V infection, while about 9% of UT7/Epo-S1 cells were B19V positive. Viral DNA detected by real-time PCR increased by more than 3 logs in CD36+ EPCs; the increase was 1 log in UT7/Epo-S1 cells. Due to the extensive permissivity of CD36+ EPCs, we significantly improved the sensitivity of detection of infectious B19V by real-time reverse transcription-PCR and IF staining 100- and 1,000-fold, respectively, which is greater than the sensitivity of UT7/Epo-S1 cell-based methods. This is the first description of an ex vivo method to produce large numbers of EPCs that are highly permissive to B19V infection and replication, offering a cellular system that mimics in vivo infection with this pathogenic human virus.

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