A decrease in the molecular weight of hyaluronic acid in synovial fluid from patients with temporomandibular disorders

Introduction. The most common way to maintain the viscoelastic properties of synovial fluid is intra-articular administration of hyaluronic acid solutions. Such forms have several features due to the method of administration, the characteristics of the substance, as well as their composition, technology, and packaging. The aim of the work to analyze the features of hyaluronic acid solutions for intra-articular administration, as well as to consider resent trends to their pharmaceutical development.Text. Currently, in Russia, most of these forms are registered as medical devices. Each drug has its characteristics, including the source of the substance, the main molecular weight and the molecular weight range of hyaluronic acid, the structure of the molecule (linear or cross-linked), the method of its chemical modification, concentration, solution volume, dosage, etc. As excipients most often use sodium chloride, water for injection, and phosphate-buffered saline to maintain pH values close to the synovial fluid. Some prostheses contain mannitol as an antioxidant. Combinations of hyaluronic acid with active chondroprotective substances (chondroitin sulfate, sodium succinate) are known. The main type of primary packaging is glass prefilled syringes. The choice of sterilization methods is determined by the chemical structure of hyaluronic acid, aseptic production is used for most prostheses.Conclusion. Currently, research solutions to create thermostable and enzyme-resistant compositions with hyaluronic acid for intra-articular administration are being successfully applied. Modern developments are aimed at creating polymer complexes of hyaluronic acid with substances that improve the lubricity of solutions, the development of nanosystems (liposomes, nanoparticles, nano micelles, etc.) with chondroprotective, as well as the creation of inert biocompatible prostheses with viscoelastic properties. The creation of forms of hyaluronic acid and alternative drugs that can support the rheological properties of synovial fluid is currently a promising area of research.

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The Role of Different Hyaluronic Acids in the Articular Cartilage of Rabbit

The Open Orthopaedics Journal ◽

10.2174/1874325001004010044 ◽

2010 ◽

Vol 4 (1) ◽

pp. 44-47 ◽

Cited By ~ 5

Author(s):

Jaime Antonio Sánchez Lázaro ◽

Pilar Coronel Granado ◽

Mercedes Gimeno del Sol ◽

Ana González Medina ◽

Luis Díaz Gállego ◽

...

Keyword(s):

Molecular Weight ◽

Hyaluronic Acid ◽

Synovial Fluid ◽

Cruciate Ligament ◽

Rheological Behaviour ◽

Study Groups ◽

Similar Molecular Weight ◽

Physico Chemical ◽

Hyaluronic Acids

Purpose: To elucidate if the differences found in the physico-chemical and rheological behaviour of Hyaluronic Acids result in different in vivo activity. For this purpose two Hyaluronic Acids (HA), HA-1 and HA-2, with similar molecular weight but different percentage of concentration variation, were compared through an osteoarthritis model. Methods and Materials: Osteoarthritis was induced in white New Zealand rabbits by anterior cruciate ligament section. After the induction period, the animals were allocated to receive HA-1 or HA-2 intra-articularly in one knee whereas the contralateral knee was used as Operated Control. An additional group of non-operated animals was used as Healthy Controls. Samples of cartilage were taken for different measures: apoptosis, nitric oxide (nitrites) and hyaluronic acid in synovial fluid. Results: The administration of HA-1 had a significant inhibitor effect on apoptosis of the chondrocytes compared to operated untreated animals (p = 0.0089), whereas this difference was not observed in the HA-2 knees. Levels of nitrites determined by HPLC in the HA-1 knees were similar to those in the Healthy group (p = 0.6551) whereas they were significantly higher in Operated Control and HA-2 groups (p = 0.0001). The comparison between HA-1 and HA-2 also revealed significantly lower levels of nitrites in the HA-1 knees (p = 0.0001). Values of hyaluronic acid in synovial fluid did not show statistical differences between the different study groups. Conclusions: HA-1 and HA-2 showed different physico-chemical characteristics and these differences have resulted in different in vivo behaviour. As a consequence, not all the HA with similar molecular weight can be considered as equivalent.

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Modern Tendencies of Synovial Fluid Substituting Drugs Based on Bound Hyaluronic Acid Use in Treatment of Patients with Loco-Motor System Pathology and Injuries

N.N. Priorov Journal of Traumatology and Orthopedics ◽

10.17816/vto20130485-91 ◽

2013 ◽

Vol 20 (4) ◽

pp. 85-91

Author(s):

M. A Strakhov ◽

A. V Skoroglyadov

Keyword(s):

Molecular Weight ◽

Hyaluronic Acid ◽

Synovial Fluid ◽

Production Technology ◽

Motor System ◽

Pain Syndrome ◽

Complex Treatment ◽

High Efficacy

Analysis of literature on hyaluronic acid role and properties as well as on the use of synovial fluid substituting drugs based on bound hyaluronic acid (HA) shows the significance of this type of therapy for the arrest of articular and periarticular pain syndrome, and high efficacy in complex treatment of osteoarthritis. Recent data enabled to understand better the mechanisms of HA metabolism and to determine the advantages HA drugs use depending on the production technology, molecular weight and other pharmacodynamics properties.

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On the interaction of hyaluronic acid with synovial fluid lipid membranes

Physical Chemistry Chemical Physics ◽

10.1039/c9cp01532a ◽

2019 ◽

Vol 21 (19) ◽

pp. 9845-9857 ◽

Cited By ~ 8

Author(s):

Paul Smith ◽

Robert M. Ziolek ◽

Elena Gazzarrini ◽

Dylan M. Owen ◽

Christian D. Lorenz

Keyword(s):

Molecular Dynamics ◽

Molecular Weight ◽

Hyaluronic Acid ◽

Synovial Fluid ◽

Molecular Dynamics Simulations ◽

Lipid Membranes ◽

Low Molecular Weight ◽

Dynamics Simulations

All-atom molecular dynamics simulations have been used to investigate the adsorption of low molecular weight hyaluronic acid to lipid membranes.

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Preparation and Characterization of the High Molecular Weight [3H]Hyaluronic Acid

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19922151 ◽

1992 ◽

Vol 57 (10) ◽

pp. 2151-2156 ◽

Cited By ~ 2

Author(s):

Peter Chabreček ◽

Ladislav Šoltés ◽

Hynek Hradec ◽

Jiří Filip ◽

Eduard Orviský

Keyword(s):

Molecular Weight ◽

Hyaluronic Acid ◽

High Molecular Weight ◽

Methyl Bromide ◽

High Performance ◽

Hydrogen Atoms ◽

Isolation And Characterization ◽

Labelling Procedure ◽

Enzymatic Depolymerization

Two methods for the preparation of high molecular weight [3H]hyaluronic acid were investigated. In the first one, hydrogen atoms in the molecule were replaced by tritium. This isotopic substitution was performed in aqueous solution using Pd/CaCO3 as the catalyst. In the second method, the high molecular weight hyaluronic acid was alkylated with [3H]methyl bromide in liquid ammonia at a temperature of -33.5 °C. High-performance gel permeation chromatographic separation method was used for the isolation and characterization of the high molecular weight [3H]hyaluronic acid. Molecular weight parameters for the labelled biopolymers were Mw = 128 kDa, Mw/Mn = 1.88 (first method) and Mw = 268 kDa, Mw/Mn = 1.55 (second method). The high molecular weight [3H]hyaluronic acid having Mw = 268 kDa was degraded further by specific hyaluronidase. Products of the enzymatic depolymerization were observed to be identical for both, labelled and cold biopolymer. This finding indicates that the described labelling procedure using [3H]methyl bromide does not induce any major structural rearrangements in the molecule.

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