scholarly journals The Role of Different Hyaluronic Acids in the Articular Cartilage of Rabbit

2010 ◽  
Vol 4 (1) ◽  
pp. 44-47 ◽  
Author(s):  
Jaime Antonio Sánchez Lázaro ◽  
Pilar Coronel Granado ◽  
Mercedes Gimeno del Sol ◽  
Ana González Medina ◽  
Luis Díaz Gállego ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Hyaluronic Acid ◽  
Synovial Fluid ◽  
Cruciate Ligament ◽  
Rheological Behaviour ◽  
Study Groups ◽  
Similar Molecular Weight ◽  
Physico Chemical ◽  
Hyaluronic Acids

Purpose: To elucidate if the differences found in the physico-chemical and rheological behaviour of Hyaluronic Acids result in different in vivo activity. For this purpose two Hyaluronic Acids (HA), HA-1 and HA-2, with similar molecular weight but different percentage of concentration variation, were compared through an osteoarthritis model. Methods and Materials: Osteoarthritis was induced in white New Zealand rabbits by anterior cruciate ligament section. After the induction period, the animals were allocated to receive HA-1 or HA-2 intra-articularly in one knee whereas the contralateral knee was used as Operated Control. An additional group of non-operated animals was used as Healthy Controls. Samples of cartilage were taken for different measures: apoptosis, nitric oxide (nitrites) and hyaluronic acid in synovial fluid. Results: The administration of HA-1 had a significant inhibitor effect on apoptosis of the chondrocytes compared to operated untreated animals (p = 0.0089), whereas this difference was not observed in the HA-2 knees. Levels of nitrites determined by HPLC in the HA-1 knees were similar to those in the Healthy group (p = 0.6551) whereas they were significantly higher in Operated Control and HA-2 groups (p = 0.0001). The comparison between HA-1 and HA-2 also revealed significantly lower levels of nitrites in the HA-1 knees (p = 0.0001). Values of hyaluronic acid in synovial fluid did not show statistical differences between the different study groups. Conclusions: HA-1 and HA-2 showed different physico-chemical characteristics and these differences have resulted in different in vivo behaviour. As a consequence, not all the HA with similar molecular weight can be considered as equivalent.

A decrease in the molecular weight of hyaluronic acid in synovial fluid from patients with temporomandibular disorders

2004 ◽  
Vol 33 (4) ◽  
pp. 224-229 ◽  
Author(s):  
Tetsu Takahashi ◽  
Kazuhiro Tominaga ◽  
Hiroshi Takano ◽  
Wataru Ariyoshi ◽  
Manabu Habu ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Hyaluronic Acid ◽  
Synovial Fluid ◽  
Temporomandibular Disorders

scholarly journals Features of Hyaluronic Acid Solutions for Intra-articular Introduction and Recent Trends in Their Development (Review)

2020 ◽  
Vol 9 (2) ◽  
pp. 45-54
Author(s):  
Y. Y. Zagorulko ◽  
E. Y. Zagorulko
Keyword(s):  
Molecular Weight ◽  
Hyaluronic Acid ◽  
Synovial Fluid ◽  
Viscoelastic Properties ◽  
Sodium Succinate ◽  
Acid Solutions ◽  
Molecular Weight Range ◽  
Polymer Complexes ◽  
Promising Area ◽  
The Creation

Introduction. The most common way to maintain the viscoelastic properties of synovial fluid is intra-articular administration of hyaluronic acid solutions. Such forms have several features due to the method of administration, the characteristics of the substance, as well as their composition, technology, and packaging. The aim of the work to analyze the features of hyaluronic acid solutions for intra-articular administration, as well as to consider resent trends to their pharmaceutical development.Text. Currently, in Russia, most of these forms are registered as medical devices. Each drug has its characteristics, including the source of the substance, the main molecular weight and the molecular weight range of hyaluronic acid, the structure of the molecule (linear or cross-linked), the method of its chemical modification, concentration, solution volume, dosage, etc. As excipients most often use sodium chloride, water for injection, and phosphate-buffered saline to maintain pH values close to the synovial fluid. Some prostheses contain mannitol as an antioxidant. Combinations of hyaluronic acid with active chondroprotective substances (chondroitin sulfate, sodium succinate) are known. The main type of primary packaging is glass prefilled syringes. The choice of sterilization methods is determined by the chemical structure of hyaluronic acid, aseptic production is used for most prostheses.Conclusion. Currently, research solutions to create thermostable and enzyme-resistant compositions with hyaluronic acid for intra-articular administration are being successfully applied. Modern developments are aimed at creating polymer complexes of hyaluronic acid with substances that improve the lubricity of solutions, the development of nanosystems (liposomes, nanoparticles, nano micelles, etc.) with chondroprotective, as well as the creation of inert biocompatible prostheses with viscoelastic properties. The creation of forms of hyaluronic acid and alternative drugs that can support the rheological properties of synovial fluid is currently a promising area of research.

Modern Tendencies of Synovial Fluid Substituting Drugs Based on Bound Hyaluronic Acid Use in Treatment of Patients with Loco-Motor System Pathology and Injuries

2013 ◽  
Vol 20 (4) ◽  
pp. 85-91
Author(s):  
M. A Strakhov ◽  
A. V Skoroglyadov
Keyword(s):  
Molecular Weight ◽  
Hyaluronic Acid ◽  
Synovial Fluid ◽  
Production Technology ◽  
Motor System ◽  
Pain Syndrome ◽  
Complex Treatment ◽  
High Efficacy

Analysis of literature on hyaluronic acid role and properties as well as on the use of synovial fluid substituting drugs based on bound hyaluronic acid (HA) shows the significance of this type of therapy for the arrest of articular and periarticular pain syndrome, and high efficacy in complex treatment of osteoarthritis. Recent data enabled to understand better the mechanisms of HA metabolism and to determine the advantages HA drugs use depending on the production technology, molecular weight and other pharmacodynamics properties.

scholarly journals Gamma-Irradiation-Prepared Low Molecular Weight Hyaluronic Acid Promotes Skin Wound Healing

Polymers ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 1214 ◽  
Author(s):  
Huang ◽  
Huang ◽  
Lew ◽  
Fan ◽  
Chang ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Wound Healing ◽  
Hyaluronic Acid ◽  
Skin Wound ◽  
Cellular Growth ◽  
Low Molecular Weight ◽  
Γ Irradiation ◽  
Skin Wound Healing

In this study, we prepared low-molecular-weight hyaluronic acid (LMWHA) powder by γ-irradiation. The chemical and physical properties of γ-irradiated LMWHA and the in vitro cellular growth experiments with γ-irradiated LMWHA were analyzed. Then, hyaluronic acid exposed to 20 kGy of γ-irradiation was used to fabricate a carboxymethyl cellulose (CMC)/LMWHA fabric for wound dressing. Our results showed that γ-irradiated LMWHA demonstrated a significant alteration in carbon–oxygen double bonding and can be detected using nuclear magnetic resonance and ultraviolet (UV)-visible (Vis) spectra. The γ-irradiated LMWHA exhibited strain rate-dependent Newton/non-Newton fluid biphasic viscosity. The viability of L929 skin fibroblasts improved upon co-culture with γ-irradiated LMWHA. In the in vivo animal experiments, skin wounds covered with dressings prepared by γ-irradiation revealed acceleration of wound healing after two days of healing. The results suggest that γ-irradiated LMWHA could be a potential source for the promotion of skin wound healing.

On the interaction of hyaluronic acid with synovial fluid lipid membranes

2019 ◽  
Vol 21 (19) ◽  
pp. 9845-9857 ◽  
Author(s):  
Paul Smith ◽  
Robert M. Ziolek ◽  
Elena Gazzarrini ◽  
Dylan M. Owen ◽  
Christian D. Lorenz
Keyword(s):  
Molecular Dynamics ◽  
Molecular Weight ◽  
Hyaluronic Acid ◽  
Synovial Fluid ◽  
Molecular Dynamics Simulations ◽  
Lipid Membranes ◽  
Low Molecular Weight ◽  
Dynamics Simulations

All-atom molecular dynamics simulations have been used to investigate the adsorption of low molecular weight hyaluronic acid to lipid membranes.

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