The fine structure of muscle fibres of roach, Rutilus rutilus (L.), and chub, Leuciscus cephalus (L.), Cyprinidae, Teleostei: interspecific differences and effects of habitat and season

This work represents the first comprehensive report on quantitative metabolomic composition of tissues of pike-perch (Sander lucioperca) and Siberian roach (Rutilus rutilus lacustris). The total of 68 most abundant metabolites are identified and quantified in the fish lenses and gills by the combination of LC-MS and NMR. It is shown that the concentrations of some compounds in the lens are much higher than that in the gills; that indicates the importance of these metabolites for the adaptation to the specific living conditions and maintaining the homeostasis of the fish lens. The lens metabolome undergoes significant seasonal changes due to the variations of dissolved oxygen level and fish feeding activity. The most season-affected metabolites are osmolytes and antioxidants, and the most affected metabolic pathway is the histidine pathway. In late autumn, the major lens osmolytes are N-acetyl-histidine and threonine phosphoethanolamine (Thr-PETA), while in winter the highest concentrations were observed for serine phosphoethanolamine (Ser-PETA) and myo-inositol. The presence of Thr-PETA and Ser-PETA in fish tissues and their role in cell osmotic protection are reported for the first time. The obtained concentrations can be used as baseline levels for studying the influence of environmental factors on fish health.

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Genetic structure and differentiation among populations of two cyprinids, Leuciscus cephalus and Rutilus rutilus, in a large European river

Canadian Journal of Fisheries and Aquatic Sciences ◽

10.1139/f99-100 ◽

1999 ◽

Vol 56 (9) ◽

pp. 1659-1667 ◽

Cited By ~ 10

Author(s):

J Laroche ◽

J D Durand ◽

Y Bouvet ◽

B Guinand ◽

B Brohon

Keyword(s):

Isolation By Distance ◽

Geographic Distance ◽

River System ◽

Rutilus Rutilus ◽

Genetic Distances ◽

Roach Rutilus Rutilus ◽

Variable Environment ◽

Environmental Diversity ◽

Historical Factors ◽

Leuciscus Cephalus

The aim of this investigation was to compare the genetic responses of two common cyprinids, chub (Leuciscus cephalus) and roach (Rutilus rutilus), to various habitat and pollution regimes within the same river basin, the Rhone, using allozyme data. Over the three main portions of the River Rhone, lower genetic polymorphism and lower heterozygosity were detected for the chub in the Saone, which is characterized by lower environmental diversity than in the Lower and Upper Rhone. On a local scale, no detectable decrease in polymorphism or heterozygosity was observed in populations of both species living in heavily polluted sites of the Rhone's Chemical Corridor. Significant differentiation was detected between populations of both chub and roach localized upstream and downstream from the dams of Donzère Mondragon, which were erected 45 years ago. The F statistics and neighbor-joining analysis of genetic distances suggested that the Upper Rhone populations were unique in comparison with the other populations for both species; this situation could be the result of historical factors and (or) a selective pressure in a variable environment. Isolation by distance was observed for chub in the less perturbed river system over a range of 100 km. By contrast, there was no relationship between geographic distance and genetic distance for roach, whatever the subsystem tested.

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