scholarly journals Growth Conditions Influence Expression of Cell Surface Hydrophobicity of Staphylococci and Other Wound Infection Pathogens

1995 ◽  
Vol 39 (10) ◽  
pp. 753-757 ◽  
Author(s):  
Åsa Ljungh ◽  
Torkel Wadström
Keyword(s):  
Cell Surface ◽  
Wound Infection ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Growth Conditions

Differential surface localization and temperature-dependent expression of the Candida albicans CSH1 protein

Microbiology ◽  
2004 ◽  
Vol 150 (2) ◽  
pp. 285-292 ◽  
Author(s):  
David R. Singleton ◽  
Kevin C. Hazen
Keyword(s):  
Candida Albicans ◽  
Cell Surface ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Growth Conditions ◽  
Northern Blotting ◽  
Temperature Dependent ◽  
Cell Surface Biotinylation ◽  
Extracellular Compartment ◽  
Primary Amino

Cell-surface hydrophobicity (CSH) in Candida albicans contributes to virulence and can be conveniently regulated in planktonic cultures by altering growth temperature. The CSH1 gene is the first candidate gene that has been demonstrated to play a role in affecting the CSH phenotype. However, the primary amino acid sequence of the CSH1 gene product suggests that the protein should be restricted to the cytoplasm. A majority of the protein appears to demonstrate that localization. Cell-surface biotinylation and limited glucanase digestion were used to determine and estimate the relative amount of Csh1p in the extracellular compartment in comparison to the cytoplasmic pool. Additionally, Western and Northern blotting were used to assess expression of the CSH1 gene under different growth conditions. Compared with cells grown at 23 °C, the total cellular levels of Csh1p are significantly greater at elevated growth temperatures. Detection of Csh1p on the cell surface correlates with the level of overall protein expression. The temperature-dependent regulation and surface presentation of Csh1p suggests a mechanism for regulating the CSH phenotype.

Effect of Growth Conditions on Flocculation and Cell Surface Hydrophobicity of Brewing Yeast

2015 ◽  
Vol 73 (2) ◽  
pp. 143-150 ◽  
Author(s):  
Jana Kopecká ◽  
Miroslav Němec ◽  
Dagmar Matoulková ◽  
Pavel Čejka ◽  
Markéta Jelínková ◽  
...  
Keyword(s):  
Cell Surface ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Growth Conditions ◽  
Brewing Yeast

Influence of oxygen limitation on the cell surface properties of bacteria from activated sludge

1998 ◽  
Vol 37 (4-5) ◽  
pp. 349-352 ◽  
Author(s):  
R. Palmgren ◽  
F. Jorand ◽  
P. H. Nielsen ◽  
J. C. Block
Keyword(s):  
Cell Surface ◽  
Activated Sludge ◽  
Surface Properties ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Growth Conditions ◽  
Oxygen Limitation ◽  
Cell Surface Properties ◽  
Number Of Factors ◽  
Math Test

Cell surface hydrophobicity is believed to be important to flocculation in activated sludge and biofilm systems. Optimization of these processes includes changes in the growth conditions of the bacteria. A number of factors influence cell surface hydrophobicity. The influence of oxygen on the cell surface hydrophobicity of 4 bacteria isolated from activated sludge was tested. The bacteria were grown in batch cultures with and without oxygen limitation. It was found that oxygen limitation generally caused a lowering of the cell surface hydrophobicity. The study also showed that there are many difficulties in measuring cell surface hydrophobicity since other cell surface properties, such as surface charge, influence the measurement methods. The MATH test was employed to establish how assay conditions influenced the results.

scholarly journals Evaluation of Relative Yeast Cell Surface Hydrophobicity Measured by Flow Cytometry

2005 ◽  
Vol 13 (1) ◽  
pp. 43-48 ◽  
Author(s):  
Lisa Colling ◽  
Richard N. Carter ◽  
Michael Essmann ◽  
Bryan Larsen
Keyword(s):  
Flow Cytometry ◽  
Cell Surface ◽  
Binding Capacity ◽  
Cell Surface Hydrophobicity ◽  
Fungal Growth ◽  
Surface Hydrophobicity ◽  
Growth Conditions ◽  
Yeast Cells ◽  
Appreciable Effect ◽  
Polystyrene Microspheres

Objective:To develop an efficient method for evaluating cell surface hydrophobicity and to apply the method to demonstrate the effects of fungal growth conditions on cell surface properties.Methods:Yeast isolates were suspended in phosphate-buffered saline and mixed with deep blue-dyed polystyrene microspheres. Flow cytometry was used to detect the degree of microsphere binding to yeast cells. Different strains of yeast were compared for intrinsic microsphere binding activity and changes in growth conditions were invoked to modify the relative surface hydrophobicity.Results:Commercially available blue-dyed polystyrene microspheres showed strong fluorescence in the FL3 channel, whereas yeast cells did not show appreciable FL3 fluorescence. Microspheres and yeast were generally distinguishable on the basis of size revealed by forward light scatter. This method showed a wide variation in intrinsic cell surface hydrophobicity amongCandida albicansstrains. Likewise, variation in hydrophobicity of non-albicans yeast species was observed. Growth on solid media, incubation at 25°C, or 250 mg/dl glucose concentration increased hydrophobicity compared with growth in liquid media, incubation at 37°C, or 50 mg/dl glucose, respectively. Growth in1×10−9M estradiol had no appreciable effect on hydrophobicity.Conclusions:Stained latex microspheres fluoresced in the FL3 channel of the flow cytometer and bound to yeast cells to an extent related to the surface hydrophobicity of the yeast. Binding detected by flow cytometry showed that clinical yeast isolates varied in intrinsic binding capacity and this binding ability was altered by different growth conditions. The implications for virulence regulation among yeast isolates are discussed.

scholarly journals Effect of ethanol on yeast film formation

OENO One ◽  
1999 ◽  
Vol 33 (1) ◽  
pp. 25
Author(s):  
Hervé Alexandre ◽  
Fanny Bertrand ◽  
Claudine Charpentier
Keyword(s):  
Cell Surface ◽  
Ethanol Concentration ◽  
Film Formation ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Growth Conditions ◽  
Adaptation Mechanism ◽  
Wine Strain ◽  
Film Development ◽  
Partition Method

<p style="text-align: justify;">In this study, we have investigated the influence of ethanol on yeast film formation and cell surface hydrophobicity (CSH). A yeast strain (P3) previously isolated from film yeast was grown in a medium containing increasing ethanol concentration ranging from 0 to 14 p. cent (v/v). It results from this study that up to 10 p. cent ethanol, the greater was the ethanol concentration, the greater was the growth of film. Using two different techniques (phase partition method, magnobead assay), we have shown that ethanol altered the CSH of the yeast. The measured hydrophobicity (p. cent) of cells grown without ethanol was 65 p. cent compared with 81 p. cent with 14 p. cent (v/v) ethanol. Taking into account the increase in CSH with increasing ethanol concentration which leads to greater film development, it seems likely that CSH alteration constitutes an adaptation mechanism which allows the cell to rise to the surface where growth conditions are favoured i.e oxydative metabolism. The role of CSH on yeast film formation was sustained by using a wine strain (3079) enable to form a film on the liquid surface, thus we have shown that this yeast possess a lower CSH (50 p. cent) compared to P3 strain (80 p. cent). However, CSH is not the only determinant for film formation since a respiratory deficient mutant (P3 <em>rho<sup>-</sup></em>) with high cell surface hydrophobicity (80 p. cent) could not form a film. Treatment of cells with lyticase which dramatically reduced CSH of P3 strain from 80 to 15 p. cent points out the protein or glycoprotein nature of the component responsible for CSH.</p>

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