scholarly journals Cross Reaction of Recombinant Equine Infectious Anemia Virus Antigen to Heterologous Strains and Application for Serological Survey among Horses in the Field

2001 ◽  
Vol 45 (1) ◽  
pp. 45-50 ◽  
Author(s):  
Hiroshi Sentsui ◽  
Yasuo Inoshima ◽  
Kenji Murakami ◽  
Hiroomi Akashi ◽  
Byamba Purevtseren ◽  
...  
Keyword(s):  
Equine Infectious Anemia Virus ◽  
Virus Antigen ◽  
Cross Reaction ◽  
Serological Survey ◽  
Equine Infectious Anemia ◽  
Anemia Virus

scholarly journals Prevalence of equine infectious anemia in stud farms in Minas Gerais, Brazil

2017 ◽  
Vol 38 (3) ◽  
pp. 1335
Author(s):  
Valéria Maria Andrade Almeida ◽  
Cairo Henrique Sousa de Oliveira ◽  
Karina Silva Fiorillo ◽  
Marilda Ferreira Martins ◽  
Rômulo Cerqueira Leite ◽  
...  
Keyword(s):  
Equine Infectious Anemia Virus ◽  
Minas Gerais ◽  
The State ◽  
Similar Distribution ◽  
Serological Survey ◽  
Incurable Disease ◽  
Potential Risk Factors ◽  
Equine Infectious Anemia ◽  
Horse Industry ◽  
Anemia Virus

Horse breeding is expanding in Brazil. Nevertheless, equine infectious anemia (EIA) a transmissible, incurable disease is an obstacle to the development of the horse industry. Therefore, to determine the incidence of EIA at stud farms in the state of Minas Gerais, Brazil, a serological survey was conducted to estimate the prevalence and identify potential risk factors for equine infectious anemia virus (EIAV) transmission. This was the second stage of an ongoing study on the epidemiology of the disease, which was first observed in draft horses. A sample of 7,742 equids from 717 stud farms in seven regions within the State was tested between May 2004 and January 2006. Laboratory tests including enzyme-linked immunosorbent assays and agar gel immunodiffusion were conducted for screening and confirmation, respectively. The prevalence of EIA was estimated to be 0.44% (95% confidence interval [CI]=0.00-0.871) at the farm level and 0.07% (95% CI=0.00-0.251) at the animal level. The low prevalence of EIA in stud farms might be explained by the higher zootechnical value of stable-bred animals, which leads to periodical serological control and compliance with the slaughter of test-positive animals in order to keep the farm EIA-free. Moreover, stable-bred horses usually travel more and are subjected to more official controls than are draft horses. The highest prevalence of EIA was observed in regions 1 (North/Northwest of Minas Gerais) and 2 (Vale do Mucuri/Jequitinhonha), with rates of 0.34% and 0.72%, respectively. These results show that the prevalence of EIA at stud farms in Minas Gerais is generally low, with the prevalence being higher in the northern part of the State (regions 1 and 2). A previous serological survey on EIA among draft horses showed a very similar distribution of EIA infection in Minas Gerais. These results suggest a very similar pattern of EIA distribution across the State and irrespective of zootechnical value, but the northern/northwestern regions have a higher prevalence than do the southern regions.

scholarly journals Purification of equine infectious anemia virus antigen by affinity chromatography

1977 ◽  
Vol 5 (6) ◽  
pp. 635-639
Author(s):  
T Sugiura ◽  
H Nakajima
Keyword(s):  
Affinity Chromatography ◽  
Equine Infectious Anemia Virus ◽  
High Sensitivity ◽  
Virus Antigen ◽  
Passive Hemagglutination ◽  
Equine Infectious Anemia ◽  
Purified Antigen ◽  
Polyethylene Glycol Precipitation ◽  
Anemia Virus ◽  
Sepharose 4B

Affinity chromatography was performed to obtain highly purified antigen from equine infectious anemia (EIA) virus. After crude antigen was concentrated by polyethylene glycol precipitation of culture fluids from equine dermal cells persistently infected with EIA virus, and after the virus was disrupted with ether, it was added to a column of cyanogen bromide-activated Sepharose 4B to which EIA-specific antibody had been conjugated. The antigen was effectively released from the column with 5M MgCl2 and proved to be highly purified. Passive hemagglutination tests on sera from EAI infections were carried out, using the purified antigen. Results indicated that the passive hemagglutination test with the antigen was a specific laboratory test with high sensitivity for EIA infection.

Preparation of equine infectious anemia virus antigen for immunodiffusion test

1973 ◽  
Vol 42 (4) ◽  
pp. 339-345 ◽  
Author(s):  
H. Nakajima ◽  
C. Ushimi ◽  
Y. Fukunaga ◽  
K. Hirasawa
Keyword(s):  
Equine Infectious Anemia Virus ◽  
Virus Antigen ◽  
Immunodiffusion Test ◽  
Equine Infectious Anemia ◽  
Anemia Virus

scholarly journals Equine infectious anemia on Marajo Island at the mouth of the Amazon river

2015 ◽  
Vol 35 (12) ◽  
pp. 947-950 ◽  
Author(s):  
Nayra F.Q.R. Freitas ◽  
Carlos M.C. Oliveira ◽  
Rômulo C. Leite ◽  
Jenner K.P. Reis ◽  
Fernanda G. Oliveira ◽  
...  
Keyword(s):  
Equine Infectious Anemia Virus ◽  
Serological Survey ◽  
Santa Cruz ◽  
Agar Gel ◽  
Incurable Disease ◽  
Island State ◽  
Equine Infectious Anemia ◽  
Marajó Island ◽  
Males And Females ◽  
Anemia Virus

Abstract: Equine infectious anemia (EIA) is a transmissible and incurable disease caused by a lentivirus, the equine infectious anemia virus (EIAV). There are no reports in the literature of this infection in Equidae on Marajo Island. The objective of this study was to diagnose the disease in the municipalities of Cachoeira do Arari, Salvaterra, Santa Cruz do Arari and Soure, on Marajó Island, state of Pará, Brazil. For serological survey samples were collected from 294 horses, over 5-month-old, males and females of puruca and marajoara breeds and from some half-breeds, which were tested by immunodiffusion in Agar gel (AGID). A prevalence of 46.26% (136/294) positive cases was found. EIA is considered endemic in the municipalities studied, due to the ecology of the region with a high numbered population of bloodsucking insect vectors and the absence of official measures for the control of the disease.

scholarly journals Evaluation of equine infectious anemia virus by the indirect ELISA EIA-LAB as screening tools in Mexico

2021 ◽  
pp. 103372
Author(s):  
Maria Carla Rodríguez Domínguez ◽  
Roberto Montes-de-Oca-Jiménez ◽  
Juan Carlos Vázquez Chagoyan ◽  
Alberto Barbabosa Pliego ◽  
Jorge Antonio Varela Guerrero ◽  
...  
Keyword(s):  
Equine Infectious Anemia Virus ◽  
Indirect Elisa ◽  
Screening Tools ◽  
Equine Infectious Anemia ◽  
Anemia Virus

scholarly journals Mapping of Equine Lentivirus Receptor 1 Residues Critical for Equine Infectious Anemia Virus Envelope Binding

2007 ◽  
Vol 82 (3) ◽  
pp. 1204-1213 ◽  
Author(s):  
Baoshan Zhang ◽  
Chengqun Sun ◽  
Sha Jin ◽  
Michael Cascio ◽  
Ronald C. Montelaro
Keyword(s):  
Equine Infectious Anemia Virus ◽  
Tumor Necrosis Factor Receptor ◽  
Binding Domain ◽  
Virus Envelope ◽  
Immunodeficiency Virus ◽  
Equine Infectious Anemia ◽  
Anemia Virus ◽  
Functional Receptor ◽  
Herpesvirus Entry Mediator ◽  
Necrosis Factor

ABSTRACT The equine lentivirus receptor 1 (ELR1), a member of the tumor necrosis factor receptor (TNFR) protein family, has been identified as a functional receptor for equine infectious anemia virus (EIAV). Toward defining the functional interactions between the EIAV SU protein (gp90) and its ELR1 receptor, we mapped the gp90 binding domain of ELR1 by a combination of binding and functional assays using the EIAV SU gp90 protein and various chimeric receptor proteins derived from exchanges between the functional ELR1 and the nonbinding homolog, mouse herpesvirus entry mediator (murine HveA). Complementary exchanges of the respective cysteine-rich domains (CRD) between the ELR1 and murine HveA proteins revealed CRD1 as the predominant determinant of functional gp90 binding to ELR1 and also to a chimeric murine HveA protein expressed on the surface of transfected Cf2Th cells. Mutations of individual amino acids in the CRD1 segment of ELR1 and murine HveA indicated the Leu70 in CRD1 as essential for functional binding of EIAV gp90 and for virus infection of transduced Cf2Th cells. The specificity of the EIAV SU binding domain identified for the ELR1 receptor is fundamentally identical to that reported previously for functional binding of feline immunodeficiency virus SU to its coreceptor CD134, another TNFR protein. These results indicate unexpected common features of the specific mechanisms by which diverse lentiviruses can employ TNFR proteins as functional receptors.

Molecular detection of equine infectious anemia virus in clinically normal, seronegative horses in an endemic area of Mexico

2021 ◽  
pp. 104063872110061
Author(s):  
César I. Romo-Sáenz ◽  
Patricia Tamez-Guerra ◽  
Aymee Olivas-Holguin ◽  
Yareellys Ramos-Zayas ◽  
Nelson Obregón-Macías ◽  
...  
Keyword(s):  
Nested Pcr ◽  
Equine Infectious Anemia Virus ◽  
Antibody Detection ◽  
Economic Losses ◽  
Clinically Normal ◽  
Pcr Product ◽  
Surveillance Programs ◽  
Equine Infectious Anemia ◽  
Anemia Virus ◽  
Equine Industry

Equine infectious anemia (EIA) is a highly infectious disease in members of the Equidae family, caused by equine infectious anemia virus (EIAV). The disease severity ranges from subclinical to acute or chronic, and causes significant economic losses in the equine industry worldwide. Serologic tests for detection of EIAV infection have some concerns given the prolonged seroconversion time. Therefore, molecular methods are needed to improve surveillance programs for this disease. We attempted detection of EIAV in 6 clinical and 42 non-clinical horses in Nuevo Leon State, Mexico, using the agar gel immunodiffusion (AGID) test for antibody detection, and nested and hemi-nested PCR for detection of proviral DNA. We found that 6 of 6, 5 of 6, and 6 of 6 clinical horses were positive by AGID, nested PCR, and hemi-nested PCR, respectively, whereas 0 of 42, 1 of 42, and 9 of 42 non-clinical horses were positive by these tests, respectively. BLAST analysis of the 203-bp 5′-LTR/ tat segment of PCR product revealed 83–93% identity with EIAV isolates in GenBank and reference strains from other countries. By phylogenetic analysis, our Mexican samples were grouped in a different clade than other sequences reported worldwide, indicating that the LRT/ tat region represents an important target for the detection of non-clinical horses.

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