Expression of corneodesmosin in the granular layer and stratum corneum of normal and diseased epidermis

The epidermis, as you know, throughout the life of the organism undergoes constant transformation: from the multiplication of basal cells, through the malpighian layer to the granular layer and, finally, to the stratum corneum, consisting of lamellar cells devoid of nucleus and protoplasm, physiologically always undergoing desquamation ... Moderate thickening of the stratum corneum of the palms and soles, as a result of the actual impact on these areas of various physical and chemical factors, is not always a pathological process.

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Trichohyalin: Presence in the Granular Layer and Stratum Corneum of Normal Human Epidermis

Journal of Investigative Dermatology ◽

10.1111/1523-1747.ep12470590 ◽

1991 ◽

Vol 96 (5) ◽

pp. 666-672 ◽

Cited By ~ 38

Author(s):

Elizabeth H. Hamilton ◽

Richard E. Payne ◽

Edward J. O'keefe

Keyword(s):

Stratum Corneum ◽

Granular Layer ◽

Human Epidermis ◽

Normal Human

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Canine papillomatosis: a retrospective study of 24 cases (2001-2011) and immunohistochemical characterization

Pesquisa Veterinária Brasileira ◽

10.1590/s0100-736x2012000700012 ◽

2012 ◽

Vol 32 (7) ◽

pp. 653-657 ◽

Cited By ~ 3

Author(s):

Matheus V. Bianchi ◽

Renata A. Casagrande ◽

Tatiane T.N. Watanabe ◽

Angelica T.B. Wouters ◽

Flademir Wouters ◽

...

Keyword(s):

Retrospective Study ◽

Stratum Corneum ◽

Granular Layer ◽

Histological Evaluation ◽

Cell Nuclei ◽

Viral Particles ◽

Mixed Breed ◽

Positive Immunostaining ◽

Epithelial Lesions ◽

Spinous Layer

A retrospective study of 24 cases of papillomas in dogs was performed from January 2001 to March 2011. Additionally, immunohistochemistry (IHC) was used to characterize and evaluate the samples. We found that disease was observed more in mixed breed dogs, ages ranging from 6 months to 10 years (mean 3.1 years), and there was no gender predilection. The main lesion sites were the skin (75%), lips (16.7%), and eyelids (8.3%). Upon histological evaluation, we observed papillary exophytic proliferation of squamous epithelium and papillary endophytic proliferation (inverted) in 87.5% and 12.5% of cases, respectively. The tumors were characterized by spinous layer hyperplasia (87.5%) with koilocytes (70.8%) and intranuclear pale basophilic inclusions bodies (8.3%), prominent granular layer with large amounts of keratohyalin granules (95.8%), and hyperkeratosis in the stratum corneum (100%). Positive immunostaining for Papillomavirus was found in 83.3% of cases, which were distributed between the granular layer and the stratum corneum. These findings indicate the following: that papillomas in dogs are caused by Papillomavirus, the viral cytopathic effect induces epithelial lesions, viral particles are found inside the cell nuclei, and inclusions bodies are rare.

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The bacteriology and histopathology of footrot in sheep

Australian Journal of Agricultural Research ◽

10.1071/ar9620725 ◽

1962 ◽

Vol 13 (4) ◽

pp. 725 ◽

Cited By ~ 17

Author(s):

JH Thomas

Keyword(s):

Epithelial Cells ◽

Stratum Corneum ◽

Granular Layer ◽

The Other ◽

Histopathological Changes ◽

Experimental Infections ◽

Sinus Formation ◽

Stratum Lucidum ◽

Pure Cultures

Fusiformis nodosus was shown to cause severe footrot in sheep without the intervention of either, or both, of the other bacteria usually present in natural cases, namely, Treponema penortha and a motile fusiform bacillus. The progress of experimental infections in the sheep's foot with pure cultures of F. nodosus has been related to the histopathological changes observed. The organism was shown to invade the stratum lucidum of the hoof but did not penetrate beyond the granular layer. The epithelial cells of the stratum lucidum became detached and disintegrated. The horn became separated from the underlying tissues in this region. Penetration into the stratum corneum was accompanied by sinus formation and degeneration of the horn.

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Study of apoptosis in normal skin tissues

Medicina ◽

10.3390/medicina43040037 ◽

2007 ◽

Vol 43 (4) ◽

pp. 301 ◽

Cited By ~ 1

Author(s):

Jūratė Grigaitienė ◽

Irena Marčiukaitienė ◽

Audra Blažienė ◽

Anželika Chomičienė

Keyword(s):

Stratum Corneum ◽

Dna Fragmentation ◽

Healthy Subjects ◽

Granular Layer ◽

Normal Skin ◽

Terminal Deoxynucleotidyl Transferase ◽

Normal Human Skin ◽

Epidermal Structure ◽

Normal Human ◽

Skin Epidermis

The aim of the study was to investigate the apoptosis in normal human skin by examination of all epidermal layers. Material and methods. The normal skin epidermis of 15 healthy subjects was investigated by TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP-X nick end labeling) technique. Apoptotic cells were evaluated in the germinative and differential compartments and stratum corneum. Only highly TUNEL-positive cells with typical morphological DNA fragmentation signs were calculated. Results. In vital strata (except stratum corneum) of normal skin epidermis, 37.5% of all TUNEL-positive cells were observed in the germinative compartment and 41.7% in the granular layer of differential compartment. Conclusions. The study showed that apoptosis occurs in all layers of normal skin epidermis. It demonstrates that apoptosis is highly important in the renewal of cells and formation of epidermal structure within all compartments.

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Expression of corneodesmosin in the granular layer and stratum corneum of normal and diseased epidermis

British Journal of Dermatology ◽

10.1046/j.1365-2133.1997.19792087.x ◽

1997 ◽

Vol 137 (6) ◽

pp. 864-873 ◽

Cited By ~ 13

Author(s):

M. HAFTEK ◽

M. SIMON ◽

J. KANITAKIS ◽

S. MARECHAL ◽

A. CLAUDY ◽

...

Keyword(s):

Stratum Corneum ◽

Granular Layer

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Changes in corneocyte element concentrations occur in skin inner stratum corneum

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100134326 ◽

1990 ◽

Vol 48 (2) ◽

pp. 146-147

Author(s):

R. R. Warner

Keyword(s):

Stratum Corneum ◽

Human Skin ◽

Element Concentration ◽

Skin Barrier ◽

Barrier Properties ◽

Brick Wall ◽

Inorganic Elements ◽

Dry Skin ◽

Skin Biopsies ◽

Intercellular Lipid

Keratinocytes undergo maturation during their transit through the viable layers of skin, and then abruptly transform into flattened, anuclear corneocytes that constitute the cellular component of the skin barrier, the stratum corneum (SC). The SC is generally considered to be homogeneous in its structure and barrier properties, and is often shown schematically as a featureless brick wall, the “bricks” being the corneocytes, the “mortar” being intercellular lipid. Previously we showed the outer SC was not homogeneous in its composition, but contained steep gradients of the physiological inorganic elements Na, K and Cl, likely originating from sweat salts. Here we show the innermost corneocytes in human skin are also heterogeneous in composition, undergoing systematic changes in intracellular element concentration during transit into the interior of the SC.Human skin biopsies were taken from the lower leg of individuals with both “good” and “dry” skin and plunge-frozen in a stirred, cooled isopentane/propane mixture.

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Paracrystalline Aggregates of Psoriatic Keratin and Their Relation to Normal Keratin Structure

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100120096 ◽

1985 ◽

Vol 43 ◽

pp. 680-681

Author(s):

S. Trachtenberg ◽

P.M. Steinert ◽

B.L. Trus ◽

A.C. Steven

Keyword(s):

Cell Death ◽

Stratum Corneum ◽

Intermediate Filament ◽

Skin Disease ◽

Amorphous Matrix ◽

Terminal Differentiation ◽

Proteolytic Degradation ◽

Horny Layer ◽

Chronic Skin ◽

Chronic Skin Disease

During terminal differentiation of vertebrate epidermis, certain specific keratin intermediate filament (KIF) proteins are produced. Keratinization of the epidermis involves cell death and disruption of the cytoplasm, leaving a network of KIF embedded in an amorphous matrix which forms the outer horny layer known as the stratum corneum. Eventually these cells are shed (desquamation). Normally, the processes of differentiation, keratinization, and desquamation are regulated in an orderly manner. In psoriasis, a chronic skin disease, a hyperkeratotic stratum corneum is produced, resulting in abnormal desquamation of unusually large scales. In this disease, the normal KIF proteins are diminished in amount or absent, and other proteins more typical of proliferative epidermal cells are present. There is also evidence of proteolytic degradation of the KIF.

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