Sustained eosinophil cationic protein release into tears after a single high-dose conjunctival allergen challenge

Background Exposure to high ambient levels of nitrogen dioxide (NO2) enhances the bronchial inflammatory reaction to allergen in humans. We tested whether this NO2 effect occurs also in the upper airways. Methods Sixteen allergic subjects with rhinitis and mild asthma were exposed at rest to either purified air or 500 μg/m3 NO2 for 30 minutes, followed 4 hours later by a nasal allergen challenge. Nasal lavage was performed before air/NO2 exposure, before allergen challenge, and 1, 4 and 18 hours after allergen challenge. Symptoms were recorded. Results The percentage of eosinophils and neutrophils, eosinophil cationic protein, and myeloperoxidase were similar after exposure to air + allergen and to NO2 + allergen. We noticed a tendency to increased sneezing the day after exposure to NO2 + allergen. Conclusion The priming effect of an ambient brief NO2 exposure on subsequent allergic response was not noticeable in activation of inflammatory cells and mediators in the upper airways.

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Effect of specific immunotherapy in eosinophil cationic protein release after specific nasal provocation

Clinical and Applied Immunology Reviews ◽

10.1016/s1529-1049(01)00014-9 ◽

2001 ◽

Vol 1 (3-4) ◽

pp. 243-248 ◽

Cited By ~ 1

Author(s):

M.Branco Ferreira ◽

S Silva ◽

M.C.Pereira dos Santos ◽

M.L.Palma Carlos ◽

A.G.Palma Carlos

Keyword(s):

Specific Immunotherapy ◽

Eosinophil Cationic Protein ◽

Protein Release ◽

Cationic Protein ◽

Nasal Provocation

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The Effect of Cetirizine on Nasal Allergic Response

American Journal of Rhinology ◽

10.2500/105065895781808739 ◽

1995 ◽

Vol 9 (6) ◽

pp. 361-366 ◽

Cited By ~ 1

Author(s):

P. Small ◽

Doreen Barrett

Keyword(s):

Late Phase ◽

Eosinophil Cationic Protein ◽

Allergen Challenge ◽

Early Response ◽

Significant Rise ◽

Phase Changes ◽

Allergic Response ◽

Response Threshold ◽

Cationic Protein ◽

Nasal Allergen Challenge

The effect of cetirizine on both early and late nasal allergic response was assessed in 15 ragweed sensitive patients. All patients were challenged before treatment and after 7 days of cetirizine 10 mg qd. Clinical assessments and measurements of mediators were performed at baseline, after positive challenge, 1 and 2 hours later, and repeated 7 and 8 hours later. There was a significant change (P < 0.0001) in the early response threshold to ragweed challenges after cetirizine. There were early phase elevations of prostaglandin D2, leukotriene C4, and eosinophil cationic protein both pre and post-treatment. Late phase changes were difficult to detect, but a statistically significant rise of both prostaglandin D2 and histamine was diminished after cetirizine treatment. Cetirizine clinically inhibited the early response to nasal allergen challenge, but early mediators were not affected. An effect on the late phase could not be clearly demonstrated.

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The kinetics of allergen-induced transcription of messenger RNA for monocyte chemotactic protein-3 and RANTES in the skin of human atopic subjects: relationship to eosinophil, T cell, and macrophage recruitment.

Journal of Experimental Medicine ◽

10.1084/jem.181.6.2153 ◽

1995 ◽

Vol 181 (6) ◽

pp. 2153-2159 ◽

Cited By ~ 98

Author(s):

S Ying ◽

L Taborda-Barata ◽

Q Meng ◽

M Humbert ◽

A B Kay

Keyword(s):

T Cells ◽

Messenger Rna ◽

Eosinophil Cationic Protein ◽

Allergen Challenge ◽

Eosinophil Infiltration ◽

Cationic Protein ◽

Monocyte Chemotactic Protein ◽

Chemotactic Protein ◽

Kinetics Of

The C-C chemokines RANTES and monocyte chemotactic protein-3 (MCP-3) are potent chemoattractants in vitro for eosinophils and other cell types associated with allergic reactions. We tested the hypothesis that the allergen-induced infiltration of eosinophils, T cells, and macrophages in the skin of atopic subjects is accompanied by the appearance of mRNA+ cells for RANTES and MCP-3. Cryostat sections were obtained from skin biopsies from six subjects 6, 24, and 48 h after allergen challenge. Tissue was processed for immunocytochemistry (ICC) and for in situ hybridization using 35S-labeled riboprobes for RANTES and MCP-3. In contrast to diluent controls, allergen provoked a significant increase in mRNA+ cells for MCP-3, which peaked at 6 h and progressively declined at 24 and 48 h. This paralleled the kinetics of total (major basic protein positive [MBP]+) and activated (cleaved form of eosinophil cationic protein [EG2]+) eosinophil infiltration. The allergen-induced expression of cells mRNA+ for RANTES was also clearly demonstrable at 6 h. However, the numbers were maximal at 24 h and declined slightly at the 48-h time point. The number of mRNA+ cells for RANTES paralleled the kinetics of infiltration of CD3+, CD4+, and CD8+ T cells whereas the number of CD68+ macrophages was still increasing at 48 h. These data support the view that MCP-3 is involved in the regulation of the early eosinophil response to specific allergen, whereas RANTES may have more relevance to the later accumulation of T cells and macrophages.

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Mechanisms of Eosinophil Cationic Protein Release in the Serum: Role of Adhesion Molecules

International Archives of Allergy and Immunology ◽

10.1159/000053597 ◽

1999 ◽

Vol 120 (1) ◽

pp. 60-64 ◽

Cited By ~ 3

Author(s):

Yoshiko Kato ◽

Takao Fujisawa ◽

Akihiko Terada ◽

Kosei Iguchi ◽

Hitoshi Kamiya

Keyword(s):

Adhesion Molecules ◽

Eosinophil Cationic Protein ◽

Protein Release ◽

Cationic Protein

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Optic Perineuritis as a Presenting Sign of Sarcoidosis

Journal of the Korean Ophthalmological Society ◽

10.3341/jkos.2021.62.7.1008 ◽

2021 ◽

Vol 62 (7) ◽

pp. 1008-1013

Author(s):

Yu Jin Roh ◽

Dong Hyun Kim ◽

Hee Kyung Yang ◽

Jeong-Min Hwang

Keyword(s):

Visual Acuity ◽

Eosinophil Cationic Protein ◽

Oral Prednisolone ◽

Serum Levels ◽

Endobronchial Ultrasound ◽

High Dose ◽

Transbronchial Needle Aspiration ◽

Cationic Protein ◽

Hilar Lymph Node ◽

Inflammatory Reactions

Purpose: To report a rare case of optic perineuritis as the presenting sign of sarcoidosis. Case summary: A 57-year-old man presented with decreased visual acuity and pain with extraocular movement in his left eye starting 2 weeks earlier. He had a history of asymptomatic optic perineuritis in that eye 18 months previously, which had improved after 1 month on oral prednisolone. His best corrected visual acuity had decreased to 20/50 and he also had relative afferent pupillary defect and color vision defects in the left eye. Slit lamp examination results were normal. There were no inflammatory reactions in the anterior chamber or vitreous. Fundoscopy showed optic disc edema. Orbital magnetic resonance imaging showed diffuse enhancement surrounding the left optic nerve. The serum levels of eosinophil cationic protein and angiotensin- converting enzyme were markedly increased. A chest radiograph showed bilateral hilar lymph node enlargement. A biopsy via endobronchial ultrasound-guided transbronchial needle aspiration revealed multifocal non-caseating granulomas. Acid fast bacilli stain showed no evidence of tuberculosis. Based on these findings, he was diagnosed with sarcoidosis. After treatment with high-dose intravenous methylprednisolone and subsequent tapering with oral prednisolone for 6 months, the visual acuity improved. Conclusions: Optic perineuritis can be the initial sign of sarcoidosis. Therefore, sarcoidosis should be considered in the differential diagnosis of optic perineuritis.

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