Poor association between allergen-specific serum immunoglobulin E levels, skin sensitivity and basophil degranulation: a study with recombinant birch pollen allergen Bet v 1 and an immunoglobulin E detection system measuring immunoglobulin E capable of binding to FceRI

The human type I allergic response is characterized by the presence of allergen-specific serum immunoglobulin E (IgE). Allergen-mediated cross-linking of receptor-bound IgE on the surface of mast cells and circulating basophils triggers the release of mediators, resulting in the development of the clinical symptoms of allergy. In order to study the structural basis of allergen–antibody interaction, a complex between the major birch-pollen allergen Bet v 1 and a Fab′ fragment isolated from the murine monoclonal Bet v 1 antibody BV16 has been crystallized. Complex crystals belong to space group P1, with unit-cell parameters a = 91.65, b = 99.14, c = 108.90 Å, α = 105.7, β = 98.32, γ = 97.62°, and diffract to 2.9 Å resolution when analyzed at 100 K using synchrotron-generated X-rays.

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Caveolar transport through nasal epithelium of birch pollen allergen Bet v 1 in allergic patients

Journal of Allergy and Clinical Immunology ◽

10.1016/j.jaci.2008.11.048 ◽

2009 ◽

Vol 124 (1) ◽

pp. 135-142.e21 ◽

Cited By ~ 33

Author(s):

Sakari Joenväärä ◽

Pirkko Mattila ◽

Jutta Renkonen ◽

Antti Mäkitie ◽

Sanna Toppila-Salmi ◽

...

Keyword(s):

Birch Pollen ◽

Pollen Allergen ◽

Nasal Epithelium ◽

Bet V 1 ◽

Birch Pollen Allergen

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Genetic Variants in CHIA and CHI3L1 Are Associated with the IgE Response to the Ascaris Resistance Marker ABA-1 and the Birch Pollen Allergen Bet v 1

PLoS ONE ◽

10.1371/journal.pone.0167453 ◽

2016 ◽

Vol 11 (12) ◽

pp. e0167453 ◽

Cited By ~ 6

Author(s):

Nathalie Acevedo ◽

Adriana Bornacelly ◽

Dilia Mercado ◽

Per Unneberg ◽

Irene Mittermann ◽

...

Keyword(s):

Genetic Variants ◽

Birch Pollen ◽

Pollen Allergen ◽

Resistance Marker ◽

Bet V 1 ◽

Birch Pollen Allergen ◽

Ige Response

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Two members of the Bet v 1 gene family encoding birch pathogenesis-related proteins display different patterns of root expression and wound-inducibility

Functional Plant Biology ◽

10.1071/pp97114 ◽

1998 ◽

Vol 25 (4) ◽

pp. 459 ◽

Cited By ~ 16

Author(s):

Pascal Poupard ◽

Désiré-Georges Strullu ◽

Philippe Simoneau

Keyword(s):

Birch Pollen ◽

Polyclonal Antiserum ◽

Pollen Allergen ◽

Pathogenesis Related Proteins ◽

Bet V 1 ◽

New Family ◽

Pathogenesis Related ◽

Birch Pollen Allergen ◽

Root Organogenesis ◽

Related Proteins

Bet v 1-SC genes constitute a subset of a multigene family in birch (Betula pendula Roth) that includes the gene for the major pollen allergen and encode proteins belonging to a new family of intracellular pathogenesis-related proteins. We have investigated the expression in birch roots of the Bet v 1-SC1 and Bet v 1-SC3 genes by northern and reverse-transcription-polymerase chain reaction analyses. Only Bet v 1-SC3 was found to be highly expressed in roots under basal conditions but this gene was not wound-inducible. Conversely the expression of the Bet v 1-SC1 gene was markedly transiently induced 8 to 9 h after the stress. Immunoblotting with polyclonal antiserum raised against the major birch pollen allergen confirmed that Bet v 1-related polypeptides are present in root extracts. This first report of Bet v 1-SC gene expression and wound-inducibility in birch roots suggests a possible function of proteins encoded by these genes in plant defense and/or root organogenesis.

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