Fluorescence analysis of copper(II) and zinc(II) binding behaviour of fulvic acids from pig slurry and amended soils

Abstract. The aim of this work was to study molecular and quantitative aspects of metal ion binding to humic substances (HS). The object of our study was characterization of two standards of humic substances (Elliott Soil standard HA 1S102H and Elliott Soil standard FA 2S102F). All samples of IHSS standards HS were characterized by elemental analysis (EA), ultraviolet-visible spectroscopy (UV/Vis), Fourier transform infrared spectroscopy (FTIR) and steady-state fluorescence spectroscopy. Chemical parameters on the complexation of Cu (II), Pb(II) and Hg(II), including the conditional stability constants and the percentage of fluorophores participating in the complexation, were estimated by the modified Stern–Volmer equation. The stability constants (log Ka) of Me(II)–ESHS complexes range from 3.70 to 5.15 in the order: Hg–ESHA>Cu–ESHA>Pb–ESHA>Cu–ESFA>Pb–ESFA. With respect to the ESHA, ESFA, which showed the smallest contents of O-containing functional groups (e.g. hydroxyl, carbonyl, ester, especially carboxyl groups on the aromatic ring) and the lowest humification degree, the ESFA was characterized by much smaller stability constants. Our findings suggest that soil HS belongs to class of important organic ligands for complexation with heavy metal ions and may significantly affect the chemical forms, mobility, bioavailability and ecotoxicity of heavy metals in the soil environment.

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Fluorescence analysis of humic and fulvic acids from two Brazilian oxisols as affected by biosolid amendment

Analytical and Bioanalytical Chemistry ◽

10.1007/s00216-005-3054-2 ◽

2005 ◽

Vol 381 (6) ◽

pp. 1281-1288 ◽

Cited By ~ 23

Author(s):

E. I. Bertoncini ◽

V. D’Orazio ◽

N. Senesi ◽

M. E. Mattiazzo

Keyword(s):

Fluorescence Analysis ◽

Fulvic Acids ◽

Humic And Fulvic Acids

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Wheat yield and leachability of phosphorus and mineral nitrogen in pig slurry amended Soils

Communications in Soil Science and Plant Analysis ◽

10.1080/00103629909370369 ◽

1999 ◽

Vol 30 (15-16) ◽

pp. 2245-2257 ◽

Cited By ~ 4

Author(s):

E. Vasconcelos ◽

F. Cabrai ◽

C.M.d.S. Cordovil

Keyword(s):

Wheat Yield ◽

Mineral Nitrogen ◽

Pig Slurry ◽

Amended Soils

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Accumulation of humic substances in an Oxisol fertilized with pig slurry for 15 years

Revista Brasileira de Engenharia Agrícola e Ambiental ◽

10.1590/1807-1929/agriambi.v25n2p109-115 ◽

2021 ◽

Vol 25 (2) ◽

pp. 109-115

Author(s):

Wagner Sacomori ◽

Paulo C. Cassol ◽

Maria S. H. Mafra ◽

Luiza F. Erdemann ◽

Jaime A. de Almeida

Keyword(s):

Humic Acid ◽

Organic Carbon ◽

Fulvic Acid ◽

Humic Substances ◽

Soil Layer ◽

Mineral Fertilizer ◽

Soil Surface ◽

Fulvic Acids ◽

Pig Slurry ◽

Continuous Application

ABSTRACT The growth of swine production in Brazil has increased the amount of production and use of pig slurry (PS) as soil fertilizer. This product provides nutrients to plants, and a continuous application of this residue usually increases total soil organic carbon (SOC) content. The objective of this study was to determine the SOC content and its distribution into humic substance fractions (humic acids, fulvic acids, and humin) in an Oxisol annually fertilized with PS for 15 years. The experiment was implemented in 2001 with a maize and oat crop rotation under no-tillage system in Campos Novos, SC, Brazil. The treatments used were: PS at rates of 0 (Control), 25, 50, 100, and 200 m3 ha-1 year-1, mineral fertilizer, and PS combined with mineral fertilizer, applied on the soil surface once a year. The 0-2.5, 2.5-5, 5-10, 10-20, 20-40, and 40-60 cm soil layers were evaluated for SOC content in the fractions humic acid, fulvic acid, and humin, and E4/E6 ratio of humic substances. Increases in PS rates up to 100 m3 ha-1 increased the SOC content in the 5-10 cm soil layer; however, decreased the proportion of the soil fulvic acid and humic acid fractions, favoring the accumulation of organic carbon in the humin fraction. The E4/E6 ratio was higher when using the soil mineral fertilizer than that found in treatments with PS.

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