Isolation of cDNAs encoding 6-phosphogluconate dehydrogenase and glucose-6-phosphate dehydrogenase from the mediterranean fruit flyCeratitis capitata: correlating genetic and physical maps of chromosome 5

1993 ◽  
Vol 1 (4) ◽  
pp. 213-222 ◽  
Author(s):  
M. J. Scott ◽  
D. Kriticou ◽  
A. S. Robinson
Keyword(s):  
Chromosome 5 ◽  
Phosphogluconate Dehydrogenase ◽  
Physical Maps ◽  
Genetic And Physical Maps ◽  
The Mediterranean ◽  
Glucose 6 Phosphate Dehydrogenase

scholarly journals Molecular Epidemiology of G6PD Genotypes in Different Ethnic Groups Residing in Saharan and Sahelian Zones of Mauritania

Pathogens ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 931
Author(s):  
Oum Kelthoum Mamadou Djigo ◽  
Mohamed Salem Ould Ahmedou Salem ◽  
Sileye Mamadou Diallo ◽  
Mohamed Abdallahi Bollahi ◽  
Boushab Mohamed Boushab ◽  
...  
Keyword(s):  
G6pd Deficiency ◽  
African Ancestry ◽  
Population Based ◽  
Black African ◽  
Plasmodium Vivax Malaria ◽  
The Mediterranean ◽  
Linguistic Groups ◽  
Study Sites ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Genotype Screening

Plasmodium vivax malaria is endemic in Mauritania. Individuals with glucose-6-phosphate dehydrogenase (G6PD) deficiency may develop acute hemolytic anemia when exposed to 8-aminoquinoline antimalarial drugs, which are indispensable for a complete cure. The prevalence of G6PD allelic variants was assessed in different ethno-linguistic groups present in Mauritania. A total of 996 blood samples (447 males and 549 females; 499 white Moors and 497 individuals of black African ancestry) were collected from febrile patients in 6 different study sites: Aleg, Atar, Kiffa, Kobeni, Nouakchott, and Rosso. The presence of the African-type G6PD A- (G202A, A376G, A542T, G680T, and T968C mutations) and the Mediterranean-type G6PD B- (C563T) variants was assessed by PCR followed by restriction fragment length polymorphism and/or DNA sequencing. The prevalence of African-type G6PD A- genotype was 3.6% (36/996), with 6.3% (28/447) of hemizygote (A-) males and 1.5% (8/549) of homozygous (A-A-) females. Forty of 549 (7.3%) women were heterozygous (AA-). The following genotypes were observed among hemizygous men and/or homozygous women: A376G/G202A (22/996; 2.2%), A376G/T968C Betica-Selma (12/996; 1.2%), and A376G/A542T Santamaria (2/996; 0.2%). The Mediterranean-type G6PD B- genotype was not observed. The prevalence rates of G6PD A- genotype in male (10/243; 4.1%) and heterozygous female (6/256; 2.3%) white Moors were lower (p < 0.05) than those of males (18/204; 8.8%) and heterozygous females (34/293; 11.6%) of black African ancestry. There were only a few homozygous women among both white Moors (3/256; 1.2%) and those of black African ancestry (5/293; 1.7%). The prevalence of G6PD deficiency in Mauritania was comparable to that of neighboring countries in the Maghreb. Because of the purportedly close ethnic ties between the Mauritanian white Moors and the peoples in the Maghreb, further investigations on the possible existence of the Mediterranean-type allele are required. Moreover, a surveillance system of G6PD phenotype and/or genotype screening is warranted to establish and monitor a population-based prevalence of G6PD deficiency.

CHANGES IN ENZYMES OF CARBOHYDRATE METABOLISM IN RAT UTERUS DURING EARLY PREGNANCY

1971 ◽  
Vol 68 (4) ◽  
pp. 805-816 ◽  
Author(s):  
M. A. H. Surani ◽  
P. J. Heald
Keyword(s):  
Energy Requirement ◽  
Lipid Synthesis ◽  
Dry Weight ◽  
Glycolytic Enzymes ◽  
Rat Uterus ◽  
Malic Dehydrogenase ◽  
Phosphogluconate Dehydrogenase ◽  
Tissue Changes ◽  
Implantation Sites ◽  
Glucose 6 Phosphate Dehydrogenase

ABSTRACT The enzymes phosphofructokinase (PFK), pyruvate kinase (PK), isocitric dehydrogenase (ICDH), malic dehydrogenase (MDH), glucose-6-phosphate dehydrogenase (G-6PDH) and 6-phosphogluconate dehydrogenase (6PGDH) have been measured in rat uterus during the first 9 days of pregnancy. It was found that after implantation on day 6, the activities of PFK and PK (the key glycolytic enzymes) increased in terms of dry weight — and in terms of protein in the implantation sites, but decreased in non-implanted tissue. The pentose shunt enzymes changed similarly to those of the glycolytic enzymes. ICDH activity increased in the non-implanted tissue and decreased in the implanted tissue. Changes in malic dehydrogenase were extremely variable and did not show a consistent pattern. Administration of Actinomycin D on day 6 of pregnancy abolished the increase in PK and PFK in the implantation sites and indeed led to a major decrease in activity. This implies that the increased PK and PFK in the implantation sites, arise from a DNA dependent RNA directed synthesis of new enzyme protein. The results are discussed in relation to the energy requirement of the decidualising tissue and the need for increased pentose for RNA synthesis. It is suggested that the extra NADPH resulting from the pentose shunt is involved in increased lipid synthesis.

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