Germ-line transformation of the Mexican fruit fly

The objective of this research was to investigate whether the medium used to transfer heat to a commodity influenced the mortality of Mexican fruit fly larvae. A similar 2-h heat dose was delivered to grapefruit via immersion in a variable temperature water bath or via exposure to a rapidly circulating gas. The concentration of oxygen and carbon dioxide inside the grapefruit was analyzed at 30-min intervals and grapefruit center temperatures recorded every 60 s during heating. The mortality of larvae located inside grapefruit during heating in a controlled atmosphere or in hot water was significantly higher than that of larvae located inside grapefruit heated in air. The internal atmosphere of grapefruit heated in a controlled atmosphere or in hot water contained significantly higher levels of carbon dioxide and lower levels of oxygen than grapefruit heated in air. Larval mortality was compared after larvae were heated in media by rapidly circulating air or by an atmosphere containing 4 kPa of oxygen and 18 kPa of carbon dioxide to evaluate whether the altered atmosphere or a heat-induced fruit metabolite was responsible for enhanced mortality. The significantly higher mortality of larvae heated in media in the presence of an altered atmosphere suggested that the altered atmosphere enhanced larval mortality. Results from this research suggest that reducing oxygen and or increasing the level of carbon dioxide during heating can enhance mortality of the Mexican fruit fly and potentially reduce the heat dose required for quarantine security.

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Economic and Highly Effective Trap–Lure Combination to Monitor the Mexican Fruit Fly (Diptera: Tephritidae) at the Orchard Level

Journal of Economic Entomology ◽

10.1093/jee/tov121 ◽

2015 ◽

Vol 108 (4) ◽

pp. 1637-1645 ◽

Cited By ~ 17

Author(s):

R. Lasa ◽

F. Herrera ◽

E. Miranda ◽

E. Gómez ◽

S. Antonio ◽

...

Keyword(s):

Fruit Fly ◽

Mexican Fruit Fly ◽

Highly Effective

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Germ-line transformation and RNAi of the ladybird beetle, Harmonia axyridis

Insect Molecular Biology ◽

10.1111/j.1365-2583.2006.00665.x ◽

2006 ◽

Vol 15 (4) ◽

pp. 507-512 ◽

Cited By ~ 26

Author(s):

H. Kuwayama ◽

T. Yaginuma ◽

O. Yamashita ◽

T. Niimi

Keyword(s):

Harmonia Axyridis ◽

Germ Line ◽

Ladybird Beetle ◽

Germ Line Transformation

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Analysis of the volatile components of a bacterial fermentation attractive to the mexican fruit fly (Anastrepha ludens).

Journal of Agricultural and Food Chemistry ◽

10.1021/jf00053a041 ◽

1995 ◽

Vol 43 (5) ◽

pp. 1348-1351 ◽

Cited By ~ 25

Author(s):

Chang-Joo Lee ◽

Albert B. DeMilo ◽

Daniel S. Moreno ◽

Adelaido J. Martinez

Keyword(s):

Fruit Fly ◽

Anastrepha Ludens ◽

Volatile Components ◽

Mexican Fruit Fly ◽

Bacterial Fermentation

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Effects of Strain and Access to Males on Female Longevity, Lifetime Oviposition Rate, and Egg Fertility of the Mexican Fruit Fly (Diptera: Tephritidae)

Journal of Economic Entomology ◽

10.1093/jee/90.4.945 ◽

1997 ◽

Vol 90 (4) ◽

pp. 945-954 ◽

Cited By ~ 13

Author(s):

Robert L. Mangan

Keyword(s):

Fruit Fly ◽

Oviposition Rate ◽

Mexican Fruit Fly ◽

Female Longevity ◽

Egg Fertility

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Probing Drosophila gene function by antisense RNA

Genome ◽

10.1139/g89-063 ◽

1989 ◽

Vol 31 (1) ◽

pp. 422-425 ◽

Cited By ~ 5

Author(s):

Reinhard Schuh ◽

Herbert Jäckle

Keyword(s):

Antisense Rna ◽

Germ Line ◽

Conventional Technique ◽

Transcription Unit ◽

The Body ◽

Mutant Phenotype ◽

Drosophila Embryo ◽

Alternative Approach ◽

Pattern Forming ◽

Germ Line Transformation

The conventional technique for assigning a particular genetic function to a cloned transcription unit has relied on the rescue of the mutant phenotype by germ line transformation. An alternative approach is to mimic a mutant phenotype by the use of antisense RNA injections to produce phenocopies. This approach has been successfully used to identify genes involved in early pattern forming processes in the Drosophila embryo. At the time when antisense RNA is injected, the embryo develops as a syncytium composed of about 5000 nuclei which share a common cytoplasm. The gene interactions required to establish the body plan occur before cellularization at the blastoderm stage. Thus the nuclei and their exported transcripts are accessible to the injected antisense RNA. The antisense RNA interferes with the endogenous RNA by an as yet unidentified mechanism. The extent of interference is only partial and produces phenocopies with characteristics of weak mutant alleles. In our lab and others, this approach has been successfully used to identify several genes required for normal Drosophila pattern formation.Key words: Drosophila segmentation, phenocopy, antisense RNA, Krüppel gene.

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