Germ-line transformation of the Queensland fruit fly, Bactrocera tryoni, using a piggyBac vector in the presence of endogenous piggyBac elements

Genetica ◽  
2010 ◽  
Vol 139 (1) ◽  
pp. 91-97 ◽  
Author(s):  
K. A. Raphael ◽  
D. C. A. Shearman ◽  
K. Streamer ◽  
J. L. Morrow ◽  
A. M. Handler ◽  
...  
2001 ◽  
Vol 10 (2) ◽  
pp. 155-162 ◽  
Author(s):  
K. Michel ◽  
A. Stamenova ◽  
A. C. Pinkerton ◽  
G. Franz ◽  
A. S. Robinson ◽  
...  

2007 ◽  
Vol 16 (5) ◽  
pp. 573-580 ◽  
Author(s):  
K. C. Condon ◽  
G. C. Condon ◽  
T. H. Dafa’alla ◽  
O. T. Forrester ◽  
C. E. Phillips ◽  
...  

2006 ◽  
Vol 15 (4) ◽  
pp. 507-512 ◽  
Author(s):  
H. Kuwayama ◽  
T. Yaginuma ◽  
O. Yamashita ◽  
T. Niimi

Genome ◽  
1989 ◽  
Vol 31 (1) ◽  
pp. 422-425 ◽  
Author(s):  
Reinhard Schuh ◽  
Herbert Jäckle

The conventional technique for assigning a particular genetic function to a cloned transcription unit has relied on the rescue of the mutant phenotype by germ line transformation. An alternative approach is to mimic a mutant phenotype by the use of antisense RNA injections to produce phenocopies. This approach has been successfully used to identify genes involved in early pattern forming processes in the Drosophila embryo. At the time when antisense RNA is injected, the embryo develops as a syncytium composed of about 5000 nuclei which share a common cytoplasm. The gene interactions required to establish the body plan occur before cellularization at the blastoderm stage. Thus the nuclei and their exported transcripts are accessible to the injected antisense RNA. The antisense RNA interferes with the endogenous RNA by an as yet unidentified mechanism. The extent of interference is only partial and produces phenocopies with characteristics of weak mutant alleles. In our lab and others, this approach has been successfully used to identify several genes required for normal Drosophila pattern formation.Key words: Drosophila segmentation, phenocopy, antisense RNA, Krüppel gene.


Author(s):  

Abstract A new distribution map is provided for Dacus tryoni[Bactrocera tryoni] (Frogg.) (Dipt., Trypetidae) (Queensland Fruit-fly) Hosts: Many deciduous and subtropical fruits. Information is given on the geographical distribution in AUSTRALIA, New South Wales, Queensland, South Australia, Victoria.


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