Technical note: Application of immobilized cells of Kluyveromyces marxianus for continuous hydrolysis to fructose of fructans in Jerusalem artichoke extracts

2007 ◽  
Vol 21 (4) ◽  
pp. 509-515 ◽  
Author(s):  
S. R. PAREKH ◽  
A. MARGARITIS
Keyword(s):  
Kluyveromyces Marxianus ◽  
Immobilized Cells ◽  
Jerusalem Artichoke ◽  
Technical Note

scholarly journals Studies on neomycin production using immobilized cells ofS marinensis NUV-5 in various reactor configurations: A technical note

2003 ◽  
Vol 4 (3) ◽  
pp. 136-141 ◽  
Author(s):  
Bandi Srinivasulu ◽  
Poluri Ellaiah ◽  
Kunamneni Adinarayana
Keyword(s):  
Immobilized Cells ◽  
Technical Note

scholarly journals “Deceived” Concentrated Immobilized Cells as Biocatalyst for Intensive Bacterial Cellulose Production from Various Sources

Catalysts ◽  
2018 ◽  
Vol 8 (1) ◽  
pp. 33 ◽  
Author(s):  
◽  
Keyword(s):  
Bacterial Cellulose ◽  
Vinyl Alcohol ◽  
Immobilized Cells ◽  
Jerusalem Artichoke ◽  
Poly Vinyl Alcohol ◽  
Cellulose Synthesis ◽  
Cellulose Production ◽  
Bacterial Cellulose Production ◽  
Renewable Biomass ◽  
Repeated Use

A new biocatalyst in the form of Komagataeibacter xylinum B-12429 cells immobilized in poly(vinyl alcohol) cryogel for production of bacterial cellulose was demonstrated. Normally, the increased bacteria concentration causes an enlarged bacterial cellulose synthesis while cells push the polysaccharide out to pack themselves into this polymer and go into a stasis. Immobilization of cells into the poly(vinyl alcohol) cryogel allowed “deceiving” them: bacteria producing cellulose pushed it out, which further passed through the pores of cryogel matrix and was accumulated in the medium while not covering the cells; hence, the latter were deprived of a possible transition to inactivity and worked on the synthesis of bacterial cellulose even more actively. The repeated use of immobilized cells retaining 100% of their metabolic activity for at least 10 working cycles (60 days) was performed. The immobilized cells produce bacterial cellulose with crystallinity and porosity similar to polysaccharide of free cells, but having improved stiffness and tensile strength. Various media containing sugars and glycerol, based on hydrolysates of renewable biomass sources (aspen, Jerusalem artichoke, rice straw, microalgae) were successfully applied for bacterial cellulose production by immobilized cells, and the level of polysaccharide accumulation was 1.3–1.8-times greater than suspended cells could produce.

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