scholarly journals Detection of Escherichia coli serogroup O103 by real-time polymerase chain reaction

2005 ◽  
Vol 98 (5) ◽  
pp. 1162-1168 ◽  
Author(s):  
S. Perelle ◽  
F. Dilasser ◽  
J. Grout ◽  
P. Fach
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Chain Reaction ◽  
Polymerase Chain

Detection of the Escherichia coli pathogenic geneeaewith three real-time polymerase chain reaction methods

2007 ◽  
Vol 53 (3) ◽  
pp. 398-403 ◽  
Author(s):  
Joanne  Karen McCrea ◽  
Chenyi Liu ◽  
Lai-King Ng ◽  
Gehua Wang
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction ◽  
Detection Limit ◽  
Real Time ◽  
Turnaround Time ◽  
Sybr Green ◽  
Sybr Green I ◽  
Chain Reaction ◽  
Culture Techniques ◽  
Polymerase Chain

Several real-time polymerase chain reaction (PCR) methods are currently available to rapidly detect the presence of a specific DNA sequence. When used for detection of pathogenic organisms, the turnaround time for PCR-based methods is much lower than for traditional culture techniques. This study compared the sensitivity of three real-time PCR methods when detecting the Escherichia coli pathogenic gene eae to determine which method is most effective in identifying very low levels of the organism. The three methods were used to detect the eae gene over a range of DNA concentrations. The differences in sensitivity were statistically significant (p < 0.05), and SYBR Green I PCR was found to have the lowest detection limit of the three; LUX primers had the highest detection limit. Therefore, using a defined DNA concentration for detecting the eae gene, SYBR Green I is the best alternative.

A Real-Time Polymerase Chain Reaction Based Assay for the Detection of Escherichia coli in Patients with Urinary Tract Infection in the Sudan

2009 ◽  
Vol 4 (4) ◽  
pp. 173-177 ◽  
Author(s):  
Humodi A. Saeed ◽  
Zahra K. Yousif ◽  
Mugahid M. El Hassan ◽  
Misk El Yamen A. Atti ◽  
Mansour M. Mansour
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Real Time ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Application of multiplex real-time polymerase chain reaction assay for simultaneous quantification of Escherichia coli virulence genes in oysters

2018 ◽  
Vol 55 (7) ◽  
pp. 2765-2773
Author(s):  
Bianca Mendes Maciel ◽  
Fernanda Tavares Bandeira de Mello ◽  
Amanda Teixeira Sampaio Lopes ◽  
Guisla Boehs ◽  
George Rêgo Albuquerque
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Polymerase Chain Reaction Assay ◽  
Virulence Genes ◽  
Chain Reaction ◽  
Simultaneous Quantification ◽  
Polymerase Chain
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