scholarly journals Development of simple and rapid PCR-fingerprinting methods forVibrio choleraeon the basis of genetic diversity of the superintegron

2010 ◽  
Author(s):  
N. Chowdhury ◽  
M. Asakura ◽  
S.B. Neogi ◽  
A. Hinenoya ◽  
S. Haldar ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Pcr Fingerprinting ◽  
Rapid Pcr ◽  
Fingerprinting Methods

scholarly journals Development of simple and rapid PCR-fingerprinting methods for Vibrio cholerae on the basis of genetic diversity of the superintegron

2009 ◽  
Author(s):  
Nityananda Chowdhury ◽  
Masahiro Asakura ◽  
Sucharit Basu Neogi ◽  
Atsushi Hinenoya ◽  
Soumya Haldar ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Vibrio Cholerae ◽  
Pcr Fingerprinting ◽  
Rapid Pcr ◽  
Fingerprinting Methods

Differentiation of Vibrio alginolyticus Strains Isolated from Sardinian Waters by Ribotyping and a New Rapid PCR Fingerprinting Method

1999 ◽  
Vol 65 (5) ◽  
pp. 1871-1875 ◽  
Author(s):  
Stefania Zanetti ◽  
Antonella Deriu ◽  
Ilaria Duprè ◽  
Maurizio Sanguinetti ◽  
Giovanni Fadda ◽  
...  
Keyword(s):  
Genetic Relationships ◽  
Restriction Enzymes ◽  
Epidemiological Studies ◽  
Vibrio Alginolyticus ◽  
Discriminative Power ◽  
Pcr Fingerprinting ◽  
Rapid Pcr ◽  
Specific Amplification ◽  
Genomic Regions ◽  
Potential Use

ABSTRACT We investigated the usefulness of a novel PCR fingerprinting technique, based on the specific amplification of genomic regions, to differentiate 30 Vibrio alginolyticus strains isolated in Sardinian waters. The different profiles obtained were scanned and analyzed by a computer program in order to determine genetic relationships. The results were then compared with the patterns obtained by ribotyping with HindIII, KpnI, andXbaI restriction enzymes. PCR fingerprinting could differentiate the strains analyzed into 12 different patterns, whereas ribotyping with XbaI, which produced the highest number of patterns, generated only 7 different profiles. This study revealed the superior discriminative power of the proposed technique for the differentiation of related V. alginolyticus strains and the potential use of PCR fingerprinting in epidemiological studies.

scholarly journals Fluorescent Amplified Fragment Length Polymorphism and Repetitive Extragenic Palindrome-PCR Fingerprinting Reveal Host-Specific Genetic Diversity of Vibrio halioticoli-Like Strains Isolated from the Gut of Japanese Abalone

2002 ◽  
Vol 68 (8) ◽  
pp. 4140-4144 ◽  
Author(s):  
Tomoo Sawabe ◽  
Fabiano L. Thompson ◽  
Jeroen Heyrman ◽  
Margo Cnockaert ◽  
Karin Hayashi ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Dna Hybridization ◽  
Fragment Length Polymorphism ◽  
Amplified Fragment Length Polymorphism ◽  
Separate Species ◽  
Pcr Fingerprinting ◽  
Abalone Species ◽  
Repetitive Extragenic Palindrome

ABSTRACT When analyzed by fluorescent amplified fragment length polymorphism and repetitive extragenic palindrome-PCR fingerprinting, a total of 47 Vibrio halioticoli strains isolated from four Japanese abalone species and one turban shell species formed three clusters that roughly reflect the different species of host abalone from which they were isolated. The V. halioticoli isolates from turban shells were distributed evenly among the clusters. Representative isolates from two clusters were deemed separate species or subspecies by DNA-DNA hybridization.

Genetic diversity and classification of cyanobacteria in different Azolla species by the use of PCR fingerprinting

1999 ◽  
Vol 99 (7-8) ◽  
pp. 1187-1193 ◽  
Author(s):  
W. W. Zheng ◽  
M. Nilsson ◽  
B. Bergman ◽  
U. Rasmussen
Keyword(s):  
Genetic Diversity ◽  
Pcr Fingerprinting

Analysis of genetic variation within clonal lineages of grape phylloxera (Daktulosphaira vitifoliae Fitch) using AFLP fingerprinting and DNA sequencing

Genome ◽  
2007 ◽  
Vol 50 (7) ◽  
pp. 660-667 ◽  
Author(s):  
S. Vorwerk ◽  
A. Forneck
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Chloroplast Dna ◽  
Aflp Fingerprinting ◽  
Daktulosphaira Vitifoliae ◽  
Banding Patterns ◽  
Grape Phylloxera ◽  
Noncoding Regions ◽  
Polymorphic Bands ◽  
Fingerprinting Methods

Two AFLP fingerprinting methods were employed to estimate the potential of AFLP fingerprints for the detection of genetic diversity within single founder lineages of grape phylloxera ( Daktulosphaira vitifoliae Fitch). Eight clonal lineages, reared under controlled conditions in a greenhouse and reproducing asexually throughout a minimum of 15 generations, were monitored and mutations were scored as polymorphisms between the founder individual and individuals of succeeding generations. Genetic variation was detected within all lineages, from early generations on. Six to 15 polymorphic loci (from a total of 141 loci) were detected within the lineages, making up 4.3% of the total amount of genetic variation. The presence of contaminating extra-genomic sequences (e.g., viral material, bacteria, or ingested chloroplast DNA) was excluded as a source of intraclonal variation. Sequencing of 37 selected polymorphic bands confirmed their origin in mostly noncoding regions of the grape phylloxera genome. AFLP techniques were revealed to be powerful for the identification of reproducible banding patterns within clonal lineages.

scholarly journals Genetic Diversity within the Population of Xanthomonas oryzae pv. oryzae in India

1997 ◽  
Vol 87 (7) ◽  
pp. 760-765 ◽  
Author(s):  
J. Yashitola ◽  
D. Krishnaveni ◽  
A. P. K. Reddy ◽  
R. V. Sonti
Keyword(s):  
Genetic Diversity ◽  
Dna Fingerprinting ◽  
Repeat Element ◽  
Xanthomonas Oryzae ◽  
Serious Disease ◽  
Fingerprinting Methods

Xanthomonas oryzae pathovar oryzae causes a serious disease of rice in India and is endemic in all of the major rice-growing areas of the country. Sixty-seven X. oryzae pv. oryzae strains, collected mostly in 1994 and 1995, from 18 locations in India were analyzed by DNA fingerprinting methods using two separate repeat element probes from the X. oryzae pv. oryzae genome. These results show that strains belonging to a single pathogen lineage can be isolated from 16 of the 18 locations sampled; many of these locations are separated from each other by hundreds of kilometers and represent ecologically diverse rice-growing areas. Pathotyping analysis indicated that the strains in this lineage belong to pathotype 1b of X. oryzae pv. oryzae.

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