Effects of Acholeplasma laidlawii and an unidentified mycoplasma on selected fish cell cultures and the replication of fish viruses

Cell lines from apparently normal gill, gonad, and kidney tissue of adult walking catfish (Clarias batrachus) were established and have been subcultured 75, 80, and 95 times, respectively. The cells were propagated in a modified Ham's F-12 medium at 25 °C but are capable of growth at 37 °C. The gill and kidney cells are the first to have been established from these tissues in fish. The kidney cell line is fibroblastic, the gonad is a mixed epithelioid–fibroblastic type, and the gill is pleomorphic. All three cell lines are susceptible to channel catfish virus (CCV), exhibiting cell fusion and other marked cytopathic effects. A live attenuated vaccine strain of CCV was produced by repeated passage of a virulent strain of CCV in kidney cell cultures. The vaccine strain is less virulent than the wild-type CCV and protects fingerling catfish against challenge with wild-type virus.Key words: channel catfish virus, Clarias batrachus, fish cell cultures, vaccine

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(Eco)toxicological effects of 2,4,7,9-tetramethyl-5-decyne-4,7-diol (TMDD) in zebrafish (Danio rerio) and permanent fish cell cultures

Environmental Science and Pollution Research ◽

10.1007/s11356-014-2806-y ◽

2014 ◽

Vol 21 (13) ◽

pp. 8233-8241 ◽

Cited By ~ 10

Author(s):

Krisztina Vincze ◽

Martin Gehring ◽

Thomas Braunbeck

Keyword(s):

Cell Cultures ◽

Danio Rerio ◽

Fish Cell ◽

Toxicological Effects

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Frequent biphasic cellular responses of permanent fish cell cultures to deoxynivalenol (DON)

Toxicology and Applied Pharmacology ◽

10.1016/j.taap.2011.07.004 ◽

2011 ◽

Vol 256 (1) ◽

pp. 24-34 ◽

Cited By ~ 20

Author(s):

Constanze Pietsch ◽

Thomas D. Bucheli ◽

Felix E. Wettstein ◽

Patricia Burkhardt-Holm

Keyword(s):

Cell Cultures ◽

Cellular Responses ◽

Fish Cell

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Infectious Pancreatic Necrosis: Selection of Virus-Free Stock from a Population of Carrier Trout

Journal of the Fisheries Research Board of Canada ◽

10.1139/f68-030 ◽

1968 ◽

Vol 25 (2) ◽

pp. 383-391 ◽

Cited By ~ 20

Author(s):

Ken Wolf ◽

M. C. Quimby ◽

C. P. Carlson ◽

G. L. Bullock

Keyword(s):

Cell Cultures ◽

Pancreatic Necrosis ◽

Control Measure ◽

Effective Control ◽

Fish Cell ◽

Specific Pathogen Free ◽

Trout Population ◽

Specific Pathogen ◽

Infectious Pancreatic Necrosis ◽

Selection Of

Infectious pancreatic necrosis (IPN) is a virulent disease of young trouts and is easily transmitted from infected animals through water and with eggs. At present, the most effective control measure consists of propagation of specific pathogen-free stock. Methods are described for using fish cell cultures to detect IPN virus in peritoneal washes, feces, and gonadal fluids and thereby to identifiy carrier and non-carrier fish. The procedures were used to derive virus-free progeny from a hatchery trout population containing about one-third carrier fish. The progeny were reared to maturity, spawned for 3 successive years, and at each spawning they proved free of virus. Recommendations and precautions are given for those who may wish to apply the procedures.

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