Rationale for the application of thin, continuous metal films in high magnification electron microscopy

1986 ◽  
Vol 142 (1) ◽  
pp. 25-34 ◽  
Author(s):  
Klaus-Ruediger Peters
Keyword(s):  
Electron Microscopy ◽  
Metal Films ◽  
High Magnification ◽  
Continuous Metal

Macromolecular structures of biological specimens are not obscured by controlled osmium impregnation

1983 ◽  
Vol 41 ◽  
pp. 606-607
Author(s):  
Klaus-Ruediger Peters ◽  
Samuel A. Green
Keyword(s):  
Thin Films ◽  
Electrical Conductivity ◽  
Critical Point ◽  
Metal Films ◽  
High Magnification ◽  
Osmium Impregnation ◽  
Instrumental Resolution ◽  
20 Nm ◽  
Continuous Metal

High magnification imaging of macromolecules on metal coated biological specimens is limited only by wet preparation procedures since recently obtained instrumental resolution allows visualization of topographic structures as smal l as 1-2 nm. Details of such dimensions may be visualized if continuous metal films with a thickness of 2 nm or less are applied. Such thin films give sufficient contrast in TEM as well as in SEM (SE-I image mode). The requisite increase in electrical conductivity for SEM of biological specimens is achieved through the use of ligand mediated wet osmiuum impregnation of the specimen before critical point (CP) drying. A commonly used ligand is thiocarbohvdrazide (TCH), first introduced to TEM for en block staining of lipids and glvcomacromolecules with osmium black. Now TCH is also used for SEM. However, after ligand mediated osinification nonspecific osmium black precipitates were often found obscuring surface details with large diffuse aggregates or with dense particular deposits, 2-20 nm in size. Thus, only low magnification work was considered possible after TCH appl ication.

scholarly journals Improving the Resolution of Sputter-coated Films

2000 ◽  
Vol 8 (2) ◽  
pp. 16-17
Author(s):  
Mary Mager
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Fine Structure ◽  
High Resolution ◽  
Carbon Film ◽  
Metal Films ◽  
Pure Gold ◽  
High Magnification ◽  
Conducting Film ◽  
Scanning Electron

After an inquiry from the Microscopy Listserver, I went back to my 1980 copy of Scanning Electron Microscopy, volume I. Several authors had investigated the structure of thin metal films by depositing the films onto carbon-film-covered TEM grids and imaging the films at high magnification. There were several proposals for new devices that have since become standards for high-resolution coaters, but the Listserver inquiry was for a fine conducting film suitabie for high-resolution SEM from an existing sputter coater.There were several factors studied that influenced the fine structure of the films. The first was the materials sputtered: for a given set of conditions of voltage, current and time, platinum gave the finest film, 60% gold-40% palladium (Au/Pd) the next finest and pure gold the least fine.

Deviations from Matthiessen's rule in continuous metal films

1996 ◽  
Vol 277 (1-2) ◽  
pp. 192-195 ◽  
Author(s):  
E. Broitman ◽  
P. Alonso ◽  
R. Zimmerman
Keyword(s):  
Metal Films ◽  
Matthiessen's Rule ◽  
Continuous Metal ◽  
Matthiessen’S Rule

scholarly journals Characterizing Magnetic Anisotropy in Amorphous Metal Films Using Tilted Fluctuation Electron Microscopy

2018 ◽  
Vol 24 (S1) ◽  
pp. 204-205
Author(s):  
Ellis Kennedy ◽  
Alejandro Ceballos ◽  
Frances Hellman ◽  
Colin Ophus ◽  
Mary Scott
Keyword(s):  
Electron Microscopy ◽  
Magnetic Anisotropy ◽  
Metal Films ◽  
Amorphous Metal ◽  
Fluctuation Electron Microscopy

Enhanced transmission of a plasmonic ellipsoid array via combining with double continuous metal films

2013 ◽  
Vol 311 ◽  
pp. 100-106 ◽  
Author(s):  
Yuanhao Chen ◽  
Guiqiang Liu ◽  
Kuan Huang ◽  
Ying Hu ◽  
Xiangnan Zhang ◽  
...  
Keyword(s):  
Metal Films ◽  
Enhanced Transmission ◽  
Continuous Metal
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