Scanning-less wide-field single-photon counting device for fluorescence intensity, lifetime and time-resolved anisotropy imaging microscopy

We describe here the development of time-correlated single-photon counting techniques from the early use of spark discharge lamps as light sources through to the use of femtosecond mode-locked lasers through the personal work of the author. We used laser-excited fluorescence in studies on energy migration and rotational relaxation in synthetic polymer solutions, in biological probe molecules and in supersonic jet expansions. Time-correlated single-photon counting was the first method used in early fluorescence lifetime imaging microscopy (FLIM), and we outline the development of this powerful technique, with a comparison of techniques including wide-field microscopy. We employed these modern forms of FLIM to study single biological cells, and applied FLIM also to gain an understanding the distribution in tissue, and fates of photosensitizer molecules used in photodynamic therapy. We also describe the uses and instrumental design of laser systems for the study of ultrafast time-resolved vibrational spectroscopy.

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Single Point PICA Probing with an Avalanche Photo-Diode

ISTFA 2001: Conference Proceedings from the 27th International Symposium for Testing and Failure Analysis ◽

10.31399/asm.cp.istfa2001p0023 ◽

2001 ◽

Author(s):

Mike Bruce ◽

Rama R. Goruganthu ◽

Shawn McBride ◽

David Bethke ◽

J.M. Chin

Keyword(s):

Single Photon ◽

Single Point ◽

Photon Counting ◽

Ring Oscillator ◽

Time Resolved ◽

Single Photon Counting ◽

Photo Diode ◽

Avalanche Photo Diode ◽

Photo Multiplier Tube ◽

Channel Plate

Abstract For time resolved hot carrier emission from the backside, an alternate approach is demonstrated termed single point PICA. The single point approach records time resolved emission from an individual transistor using time-correlated-single-photon counting and an avalanche photo-diode. The avalanche photo-diode has a much higher quantum efficiency than micro-channel plate photo-multiplier tube based imaging cameras typically used in earlier approaches. The basic system is described and demonstrated from the backside on a ring oscillator circuit.

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UVscope and its application aboard the ASTRI-Horn telescope

Experimental Astronomy ◽

10.1007/s10686-021-09728-6 ◽

2021 ◽

Author(s):

Maria Concetta Maccarone ◽

Giovanni La Rosa ◽

Osvaldo Catalano ◽

Salvo Giarrusso ◽

Alberto Segreto ◽

...

Keyword(s):

Gamma Ray ◽

Single Photon ◽

Photon Counting ◽

Light Flux ◽

Cosmic Ray ◽

High Energy ◽

Wide Field ◽

High Energy Astrophysics ◽

Single Photon Counting ◽

Photon Counting Mode

AbstractUVscope is an instrument, based on a multi-pixel photon detector, developed to support experimental activities for high-energy astrophysics and cosmic ray research. The instrument, working in single photon counting mode, is designed to directly measure light flux in the wavelengths range 300-650 nm. The instrument can be used in a wide field of applications where the knowledge of the nocturnal environmental luminosity is required. Currently, one UVscope instrument is allocated onto the external structure of the ASTRI-Horn Cherenkov telescope devoted to the gamma-ray astronomy at very high energies. Being co-aligned with the ASTRI-Horn camera axis, UVscope can measure the diffuse emission of the night sky background simultaneously with the ASTRI-Horn camera, without any interference with the main telescope data taking procedures. UVscope is properly calibrated and it is used as an independent reference instrument for test and diagnostic of the novel ASTRI-Horn telescope.

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