Postharvest temperature and light treatments induce anthocyanin accumulation in peel of ‘Akihime’ plum (Prunus salicina Lindl.) via transcription factor PsMYB10.1

Abstract Anthocyanins are preferentially accumulated in certain tissues of particular species of citrus. A R2R3-MYB transcription factor (named Ruby1) has been well documented as an activator of citrus anthocyanin biosynthesis. In this study, we characterized CsMYB3, a transcriptional repressor that regulates anthocyanin biosynthesis in citrus. CsMYB3 was expressed in anthocyanin-pigmented tissues, and the expression was closely associated with that of Ruby1, which is a key anthocyanin activator. Overexpression of CsMYB3 in Arabidopsis resulted in a decrease in anthocyanins under nitrogen stress. Overexpression of CsMYB3 in the background of CsRuby1-overexpressing strawberry and Arabidopsis reduced the anthocyanin accumulation level. Transient promoter activation assays revealed that CsMYB3 could repress the activation capacity of the complex formed by CsRuby1/CsbHLH1 for the anthocyanin biosynthetic genes. Moreover, CsMYB3 could be transcriptionally activated by CsRuby1 via promoter binding, thus forming an ‘activator-and-repressor’ loop to regulate anthocyanin biosynthesis in citrus. This study shows that CsMYB3 plays a repressor role in the regulation of anthocyanin biosynthesis and proposes an ‘activator-and-repressor’ loop model constituted by CsRuby1 and CsMYB3 in the regulation of anthocyanin biosynthesis in citrus.

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Transcription Factor Families Regulate the Anthocyanin Biosynthetic Pathway in Capsicum annuum

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.134.2.244 ◽

2009 ◽

Vol 134 (2) ◽

pp. 244-251 ◽

Cited By ~ 31

Author(s):

John R. Stommel ◽

Gordon J. Lightbourn ◽

Brenda S. Winkel ◽

Robert J. Griesbach

Keyword(s):

Transcription Factor ◽

Differential Expression ◽

Capsicum Annuum ◽

Structural Gene ◽

Cdna Clones ◽

Gene Promoters ◽

Anthocyanin Accumulation ◽

Transcript Levels ◽

Tissue Specific ◽

Foliar Tissue

Anthocyanin structural gene transcription requires the expression of at least one member of each of three transcription factor families: MYC, MYB, and WD40. These transcription factors form a complex that binds to structural gene promoters, thereby modulating gene expression. Capsicum annuum L. (pepper) displays a wide spectrum of tissue-specific anthocyanin pigmentation, making it a useful model for the study of anthocyanin accumulation. To determine the genetic basis for tissue-specific pigmentation, we used real-time polymerase chain reaction to evaluate the expression of anthocyanin biosynthetic (Chs, Dfr, and Ans) and regulatory (Myc, MybA , and Wd) genes in flower, fruit, and foliar tissue from pigmented and nonpigmented C. annuum genotypes. No differences were observed in expression of the Wd gene among these tissues. However, in all cases, biosynthetic gene transcript levels were significantly higher in anthocyanin-pigmented tissue than in nonpigmented tissues. MybA and Myc transcript levels were also substantially higher in anthocyanin-pigmented floral and fruit tissues. Our results demonstrate that differential expression of C. annuum MybA as well as Myc occurs coincident with anthocyanin accumulation in C. annuum flower and fruit tissues. In contrast to the situation in flowers and fruit, differential expression of MybA and Myc was not observed in foliar tissue, suggesting that different mechanisms contribute to the regulation of anthocyanin biosynthesis in different parts of the C. annuum plant. Cloning and sequencing of MybA genomic and cDNA clones revealed two introns of 249 and 441 bp between the R2R3 domains. Whereas the Myb R2R3 domains were conserved between C. annuum and Petunia ×hybrida Vilm., the sequence of the non-R2R3 domains was not conserved, with very little homology in these related Solanaceous species.

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The bZIP transcription factor MdHY5 regulates anthocyanin accumulation and nitrate assimilation in apple

Horticulture Research ◽

10.1038/hortres.2017.23 ◽

2017 ◽

Vol 4 (1) ◽

Cited By ~ 57

Author(s):

Jian-Ping An ◽

Feng-Jia Qu ◽

Ji-Fang Yao ◽

Xiao-Na Wang ◽

Chun-Xiang You ◽

...

Keyword(s):

Transcription Factor ◽

Nitrate Reductase ◽

Leucine Zipper ◽

Anthocyanin Biosynthesis ◽

Nitrate Assimilation ◽

Bzip Transcription Factor ◽

Anthocyanin Accumulation ◽

Mobility Shift ◽

Basic Leucine Zipper ◽

Transgenic Apple

Abstract The basic leucine zipper (bZIP) transcription factor HY5 plays a multifaceted role in plant growth and development. Here the apple MdHY5 gene was cloned based on its homology with Arabidopsis HY5. Expression analysis demonstrated that MdHY5 transcription was induced by light and abscisic acid treatments. Electrophoretic mobility shift assays and transient expression assays subsequently showed that MdHY5 positively regulated both its own transcription and that of MdMYB10 by binding to E-box and G-box motifs, respectively. Furthermore, we obtained transgenic apple calli that overexpressed the MdHY5 gene, and apple calli coloration assays showed that MdHY5 promoted anthocyanin accumulation by regulating expression of the MdMYB10 gene and downstream anthocyanin biosynthesis genes. In addition, the transcript levels of a series of nitrate reductase genes and nitrate uptake genes in both wild-type and transgenic apple calli were detected. In association with increased nitrate reductase activities and nitrate contents, the results indicated that MdHY5 might be an important regulator in nutrient assimilation. Taken together, these results indicate that MdHY5 plays a vital role in anthocyanin accumulation and nitrate assimilation in apple.

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Loss of the R2R3 MYB Transcription Factor RsMYB1 Shapes Anthocyanin Biosynthesis and Accumulation in Raphanus sativus

International Journal of Molecular Sciences ◽

10.3390/ijms222010927 ◽

2021 ◽

Vol 22 (20) ◽

pp. 10927

Author(s):

Da-Hye Kim ◽

Jundae Lee ◽

JuHee Rhee ◽

Jong-Yeol Lee ◽

Sun-Hyung Lim

Keyword(s):

Transcription Factor ◽

Raphanus Sativus ◽

Anthocyanin Biosynthesis ◽

Antioxidant Properties ◽

Myb Transcription Factor ◽

Anthocyanin Accumulation ◽

Truncated Protein ◽

Frame Shift ◽

Frame Shift Mutation ◽

R2r3 Myb

The red or purple color of radish (Raphanus sativus L.) taproots is due to anthocyanins, which have nutritional and aesthetic value, as well as antioxidant properties. Moreover, the varied patterns and levels of anthocyanin accumulation in radish roots make them an interesting system for studying the transcriptional regulation of anthocyanin biosynthesis. The R2R3 MYB transcription factor RsMYB1 is a key positive regulator of anthocyanin biosynthesis in radish. Here, we isolated an allele of RsMYB1, named RsMYB1Short, in radish cultivars with white taproots. The RsMYB1Short allele carried a 4 bp insertion in the first exon causing a frame-shift mutation of RsMYB1, generating a truncated protein with only a partial R2 domain at the N-terminus. Unlike RsMYB1Full, RsMYB1Short was localized to the nucleus and the cytoplasm and failed to interact with their cognate partner RsTT8. Transient expression of genomic or cDNA sequences for RsMYB1Short in radish cotyledons failed to induce anthocyanin accumulation, but that for RsMYB1Full activated it. Additionally, RsMYB1Short showed the lost ability to induce pigment accumulation and to enhance the transcript level of anthocyanin biosynthetic genes, while RsMYB1Full promoted both processes when co-expressed with RsTT8 in tobacco leaves. As the result of the transient assay, co-expressing RsTT8 and RsMYB1Full, but not RsMYB1Short, also enhanced the promoter activity of RsCHS and RsDFR. We designed a molecular marker for RsMYB1 genotyping, and revealed that the RsMYB1Short allele is common in white radish cultivars, underscoring the importance of variation at the RsMYB1 locus in anthocyanin biosynthesis in the radish taproot. Together, these results indicate that the nonsense mutation of RsMYB1 generated the truncated protein, RsMYB1Short, that had the loss of ability to regulate anthocyanin biosynthesis. Our findings highlight that the frame shift mutation of RsMYB1 plays a key role in anthocyanin biosynthesis in the radish taproot.

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The Photomorphogenic Transcription Factor PpHY5 Regulates Anthocyanin Accumulation in Response to UVA and UVB Irradiation

Frontiers in Plant Science ◽

10.3389/fpls.2020.603178 ◽

2021 ◽

Vol 11 ◽

Author(s):

Yun Zhao ◽

Ting Min ◽

Miaojin Chen ◽

Hongxun Wang ◽

Changqing Zhu ◽

...

Keyword(s):

Transcription Factor ◽

Uv Irradiation ◽

White Light ◽

Chalcone Synthase ◽

Anthocyanin Biosynthesis ◽

Light Signaling ◽

Anthocyanin Content ◽

Anthocyanin Accumulation ◽

Uvb Irradiation ◽

Genes Encoding

Red coloration contributes to fruit quality and is determined by anthocyanin content in peach (Prunus persica). Our previous study illustrated that anthocyanin accumulation is strongly regulated by light, and the effect of induction differs according to light quality. Here we showed that both ultraviolet-A (UVA) and ultraviolet-B (UVB) irradiation promoted anthocyanin biosynthesis in “Hujingmilu” peach fruit, and a combination of UVA and UVB had additional effects. The expression of anthocyanin biosynthesis and light signaling related genes, including transcription factor genes and light signaling elements, were induced following UV irradiation as early as 6 h post-treatment, earlier than apparent change in coloration which occurred at 72 h. To investigate the molecular mechanisms for UVA- and UVB-induced anthocyanin accumulation, the genes encoding ELONGATED HYPOCOTYL 5 (HY5), CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1), Cryptochrome (CRY), and UV RESISTANCE LOCUS 8 (UVR8) in peach were isolated and characterized through functional complementation in corresponding Arabidopsis (Arabidopsis thaliana) mutants. PpHY5 and PpCOP1.1 restored hypocotyl length and anthocyanin content in Arabidopsis mutants under white light; while PpCRY1 and PpUVR8.1 restored AtHY5 expression in Arabidopsis mutants in response to UV irradiation. Arabidopsis PpHY5/hy5 transgenic lines accumulated higher amounts of anthocyanin under UV supplementation (compared with weak white light only), especially when UVA and UVB were applied together. These data indicated that PpHY5, acting as AtHY5 counterpart, was a vital regulator in UVA and UVB signaling pathway. In peach, the expression of PpHY5 was up-regulated by UVA and UVB, and PpHY5 positively regulated both its own transcription by interacting with an E-box in its own promoter, and the transcription of the downstream anthocyanin biosynthetic genes chalcone synthase 1 (PpCHS1), chalcone synthase 2 (PpCHS2), and dihydroflavonol 4-reductase (PpDFR1) as well as the transcription factor gene PpMYB10.1. In summary, functional evidence supports the role of PpHY5 in UVA and UVB light transduction pathway controlling anthocyanin biosynthesis. In peach this is via up-regulation of expression of genes encoding biosynthetic enzymes, as well as the transcription factor PpMYB10.1 and PpHY5 itself.

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Correction: Constitutive expression of an A-5 subgroup member in the DREB transcription factor subfamily from Ammopiptanthus mongolicus enhanced abiotic stress tolerance and anthocyanin accumulation in transgenic Arabidopsis

PLoS ONE ◽

10.1371/journal.pone.0227290 ◽

2019 ◽

Vol 14 (12) ◽

pp. e0227290

Author(s):

Meiyan Ren ◽

Zhilin Wang ◽

Min Xue ◽

Xuefeng Wang ◽

Feng Zhang ◽

...

Keyword(s):

Transcription Factor ◽

Abiotic Stress ◽

Stress Tolerance ◽

Transgenic Arabidopsis ◽

Abiotic Stress Tolerance ◽

Constitutive Expression ◽

Anthocyanin Accumulation ◽

Ammopiptanthus Mongolicus ◽

Dreb Transcription Factor ◽

Subgroup Member

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Genome-wide identification and expression analysis of the MYB transcription factor in Japanese plum (Prunus salicina)

Genomics ◽

10.1016/j.ygeno.2020.08.018 ◽

2020 ◽

Vol 112 (6) ◽

pp. 4875-4886

Author(s):

Chaoyang Liu ◽

Jingjing Hao ◽

Mengqing Qiu ◽

Jianjun Pan ◽

Yehua He

Keyword(s):

Transcription Factor ◽

Expression Analysis ◽

Myb Transcription Factor ◽

Japanese Plum ◽

Prunus Salicina ◽

Genome Wide

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FvbHLH9 Functions as a Positive Regulator of Anthocyanin Biosynthesis by Forming a HY5–bHLH9 Transcription Complex in Strawberry Fruits

Plant and Cell Physiology ◽

10.1093/pcp/pcaa010 ◽

2020 ◽

Vol 61 (4) ◽

pp. 826-837 ◽

Cited By ~ 4

Author(s):

Yang Li ◽

Pengbo Xu ◽

Guanqun Chen ◽

Jun Wu ◽

Zhongchi Liu ◽

...

Keyword(s):

Transcription Factor ◽

Promoter Region ◽

Anthocyanin Biosynthesis ◽

Anthocyanin Synthesis ◽

Bhlh Transcription Factor ◽

Anthocyanin Accumulation ◽

Positive Regulator ◽

Key Enzyme ◽

Synergistic Regulation ◽

Transient Overexpression

Abstract Anthocyanin accumulation is transcriptionally regulated by the MYB–bHLH–WD40 complex. Light is indispensable for anthocyanin accumulation, and light-inducible MYB and HY5 were considered to promote anthocyanin accumulation in many fruits. Whether and how light-inducible bHLH transcription factor and HY5 regulate anthocyanin synthesis in strawberry is unknown. In this study, we identified a bHLH transcription factor, FvbHLH9, which was induced by light as well as FvHY5, and found that, similar to FvHY5, the transient overexpression and interference FvbHLH9 in strawberry fruits can promote and decrease anthocyanin accumulation, respectively, indicating FvbHLH9 functions as a positive regulator of anthocyanin biosynthesis. Furthermore, we confirmed that both FvHY5 and FvbHLH9 specifically bind to the promoter region of some key enzyme genes, including FvDFR, and the expression of FvDFR was activated through the heterodimer formation between FvHY5 and FvbHLH9. Finally, we confirmed that FvbHLH9-promoted anthocyanin accumulation is dependent on HY5–bHLH heterodimerisation in Arabidopsis. Our findings provide insights into a mechanism involving the synergistic regulation of light-dependent coloration and anthocyanin biosynthesis via a HY5–bHLH heterodimer formed by the interaction of FvHY5 and FvbHLH9 in strawberry fruits.

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