Tissue Typing of Cells in Culture. IV. Cell Surface Antigens of Tumor Cell Lines, not Obviously Belonging to the HLA-System

1978 ◽  
Vol 11 (3) ◽  
pp. 287-294 ◽  
Author(s):  
Åke Espmark
Keyword(s):  
Cell Surface ◽  
Tumor Cell ◽  
Cell Lines ◽  
Surface Antigens ◽  
Tumor Cell Lines ◽  
Cell Surface Antigens ◽  
Tissue Typing ◽  
Hla System ◽  
Cells In Culture

Surface Antigens on Transplantable Tumor Cell Lines Producing Mouse Type C Viruses

1977 ◽  
Vol 58 (4) ◽  
pp. 1069-1078 ◽  
Author(s):  
Tadao Aoki ◽  
Ronald B. Herberman ◽  
Janet W. Hartley ◽  
Margaret Liu ◽  
Mary Jane Walling ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cell Lines ◽  
Surface Antigens ◽  
Tumor Cell Lines ◽  
Transplantable Tumor ◽  
Type C

scholarly journals ALPPL2 is a highly specific and targetable tumor cell surface antigen

2020 ◽  
Author(s):  
Yang Su ◽  
Xin Zhang ◽  
Scott Bidlingmaier ◽  
Christopher R. Behrens ◽  
Nam-Kyung Lee ◽  
...  
Keyword(s):  
Cell Surface ◽  
Tumor Cell ◽  
Surface Antigen ◽  
Tissue Specificity ◽  
Surface Antigens ◽  
Cell Surface Antigen ◽  
Cell Surface Antigens ◽  
Normal Tissues ◽  
Specific Tumor ◽  
Therapy Development

AbstractIt has been challenging to identify tumor-specific cell surface antigens as the vast majority of tumor-associated antigens are also expressed by some normal tissues. In the course of our study on mesothelioma, we identified a highly specific tumor cell surface antigen that can be targeted for therapy development. Mesothelioma is caused by malignant transformation of the mesothelium, incurable and categorized into three histological subtypes, epithelioid, biphasic and sarcomatoid. To identity novel mesothelioma cell surface antigens with broad subtype coverage and high tissue specificity, we have previously selected phage antibody display libraries on live mesothelioma cells and tissues following counter-selection on normal cells, and identified a panel of human antibodies that bind all subtypes of mesothelioma but not normal mesothelium. One of the antibodies, M25, showed high specificity, and we hereby report the identification of the M25 antigen as ALPPL2. We performed immunohistochemistry on normal human tissues and found that ALPPL2 is expressed only on placental trophoblasts but not any other normal tissues. This exquisite tissue specificity and broad tumor type coverage suggests that ALPPL2 could be an excellent cell surface target for therapeutic development against mesothelioma. To evaluate therapeutic potential of ALPPL2 targeting, we developed an ALPPL2-targeted antibody-drug conjugate and demonstrated potent and specific tumor killing in vitro and in vivo against both epithelioid and sarcomatoid mesothelioma. Thus ALPPL2 belongs to a rare class of cell surface antigens that can be said as being truly tumor specific and is well suited for therapy development against ALPPL2 expressing tumors.

scholarly journals Cell surface antigens of chemically induced sarcomas of the mouse. I. Murine leukemia virus-related antigens and alloantigens on cultured fibroblasts and sarcoma cells: description of a unique antigen on BALB/c Meth A sarcoma.

1977 ◽  
Vol 146 (3) ◽  
pp. 720-734 ◽  
Author(s):  
A B DeLeo ◽  
H Shiku ◽  
T Takahashi ◽  
M John ◽  
L J Old
Keyword(s):  
Cell Surface ◽  
Cell Lines ◽  
Murine Leukemia Virus ◽  
Leukemia Virus ◽  
Surface Antigens ◽  
Cell Surface Antigens ◽  
Cultured Fibroblasts ◽  
Chemically Induced ◽  
Sarcoma Cells ◽  
Murine Leukemia

As background for a serological definition of the unique antigens of chemically induced sarcomas, we have typed a series of fibroblast and sarcoma cell lines of BALB/c and C57BL/6 origin by cytoxicity and absorption tests for murine leukemia virus (MuLV)-related cell surface antigens and known alloantigens. 7 of the 17 cultured lines expressed the range of cell surface antigens associated with MuLV (GIX, GCSA, gp70, p30), and this was invariably associated with MuLV production. In nonproducer lines of C57BL/6 (but not BALB/c) origin, a MuLV-gp70-like molecule was found on the surface of fibroblasts and sarcoma cells. The alloantigenic phenotype of these MuLV+ and MuLV- cell lines was H-2D+, H-2K+, Thy-1.2+ or -, PC.1+ or -, Lyt-1.2-, Lyt-2.2-, Ia.7-, and TL.2-. A unique antigen was defined on the BALB/c ascites sarcoma Meth A with antisera prepared in BALB/c or (BALB/c X C57BL/6)F1 mice. Tissue culture lines derived from this tumor were MuLV-, which facilitated serological study of the antigen. Absorption analysis indicated that the antigen was restricted to Meth A; it could not be detected in normal or fetal BALB/c tissue MuLV+ or MuLV- fibroblast lines, 12 syngeneic or allogeneic sarcomas, or normal lymphoid cells from 13 different inbred mouse strains.

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