Characterization of hexose kinases from camellia and lily pollen grains

The pollen morphology of thirteen species of Cactaceae was studied: M. backebergiana F.G. Buchenau, M. decipiens Scheidw, M. elongata DC, M. gracilis Pfeiff., M. hahniana Werderm., M. marksiana Krainz, M. matudae Bravo, M. nejapensis R.T. Craig & E.Y. Dawson, M. nivosa Link ex Pfeiff., M. plumosa F.A.C. Weber, M. prolifera (Mill.) Haw, M. spinosissima var. “A Peak” Lem. and M. voburnensis Scheer. All analysed pollen grains are monads, with radial symmetry, medium size (M. gracilis, M. marksiana, M. prolifera, large), tricolpates (dimorphs in M. plumosa [3-6 colpus] and M. prolifera [3-6 colpus]), with circular-subcircular amb (quadrangular in M. prolifera and M. plumosa with six colpus). The pollen grains presented differences in relation to the shape and exine thickness. The exine was microechinate and microperforated. The pollen morphological data are unpublished and will aid in studies that use pollen samples. These pollen grains indicate ornamental cacti.

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Characterization of a Desiccation-Related Protein in Lily Pollen during Development and Stress

Plant and Cell Physiology ◽

10.1093/oxfordjournals.pcp.a029335 ◽

1998 ◽

Vol 39 (12) ◽

pp. 1307-1314 ◽

Cited By ~ 37

Author(s):

C.-S. Wang ◽

Y.-E. Liau ◽

J.-C. Huang ◽

T.-D. Wu ◽

C.-C. Su ◽

...

Keyword(s):

Related Protein ◽

Lily Pollen

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Reproductive characterization of interspecific hybrids among Capsicum species

Cropp Breeding and Applied Biotechnology ◽

10.1590/s1984-70332011000300006 ◽

2011 ◽

Vol 11 (3) ◽

pp. 241-249 ◽

Cited By ~ 11

Author(s):

Carlos Eduardo da Silva Monteiro ◽

Telma Nair Santana Pereira ◽

Karina Pereira de Campos

Keyword(s):

Male Sterility ◽

Interspecific Hybrids ◽

Pollen Viability ◽

Pollen Grains ◽

Male Sterile ◽

Viable Pollen ◽

Hybrid Combination ◽

Capsicum Species ◽

The Cross

The objective of this study was the reproductive characterization of Capsicum accessions as well as of interspecific hybrids, based on pollen viability. Hybrids were obtained between Capsicum species. Pollen viability was high in most accessions, indicating that meiosis is normal, resulting in viable pollen grains. The pollen viability of species C. pubescens was the lowest (27 %). The interspecific hybrids had varying degrees of pollen viability, from fertile combinations (C. chinense x C. frutescens and C. annuum x C. baccatum) to male sterile combinations. Pollen viability also varied within the hybrid combination according to accessions used in the cross. Results indicate that male sterility is one of the incompatibility barriers among Capsicum species since hybrids can be established, but may be male sterile.

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snRNP: Rich Nuclear Bodies inHyacinthus orientalisL. Microspores and Developing Pollen Cells

International Journal of Cell Biology ◽

10.1155/2009/209303 ◽

2009 ◽

Vol 2009 ◽

pp. 1-12 ◽

Cited By ~ 4

Author(s):

K. Zienkiewicz ◽

E. Bednarska

Keyword(s):

Pollen Development ◽

Rna Synthesis ◽

Generative Cell ◽

Pollen Grains ◽

Strong Relationship ◽

Nuclear Bodies ◽

Cajal Body ◽

Sm Proteins ◽

Hyacinthus Orientalis

The aim of the present work was the characterization of nuclear bodies in the microspore and developing pollen cells ofHyacinthus orientalisL.. The combination of Ag-NOR, immunofluorescence and immunogold techniques was used in this study. The obtained results showed the presence of highly agyrophylic extranucleolar bodies in microspore and developing pollen cells, which were finally identified as Cajal bodies. In all cases, a strong accumulation of snRNP-indicating molecules including TMG cap, Sm proteins and U2 snRNA, was observed in the examined nuclear bodies. In contrast to their number the size of the identified structures did not change significantly during pollen development. In the microspore and the vegetative cell of pollen grains CBs were more numerous than in the generative cell. At later stages of pollen development, a drastic decrease in CB number was observed and, just before anthesis, a complete lack of these structures was indicated in both pollen nuclei. On the basis of these results, as well as our previous studies, we postulate a strong relationship between Cajal body numbers and the levels of RNA synthesis and splicing machinery elements in microspore and developing pollen cells.

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Isolation and Characterization of Viable Sperm Cells from Tricellular Pollen Grains

Sexual Reproduction in Higher Plants ◽

10.1007/978-3-642-73271-3_39 ◽

1988 ◽

pp. 245-250 ◽

Cited By ~ 2

Author(s):

E. Matthys-Rochon ◽

S. Detchepare ◽

V. Wagner ◽

P. Roeckel ◽

C. Dumas

Keyword(s):

Pollen Grains ◽

Sperm Cells ◽

Isolation And Characterization ◽

Viable Sperm

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Molecular analysis of the maize wx-B3 allele indicates that precise excision of the transposable Ac element is rare.

Genetics ◽

10.1093/genetics/130.2.377 ◽

1992 ◽

Vol 130 (2) ◽

pp. 377-384 ◽

Cited By ~ 1

Author(s):

G Baran ◽

C Echt ◽

T Bureau ◽

S Wessler

Keyword(s):

Insertion Site ◽

Pollen Grains ◽

Genetic Maps ◽

Null Alleles ◽

P Elements ◽

Ac Element ◽

Precise Excision ◽

Wx Gene ◽

Low Levels

Abstract The somatic and germinal behavior of the maize wx-B3 mutation indicates that this Ac allele rarely reverts. Endosperms containing wx-B3 display tiny and infrequent Wx revertant sectors while no significant reversion is detected when wx-B3 pollen is stained with I/KI. Previous studies of other transposable element alleles that revert infrequently have implicated low levels of element excision. Unlike these other alleles, the wx-B3 Ac element is indistinguishable from fully active Ac elements with respect to its structure, and its ability to transpose from the Wx gene or to trans-activate a Ds element. Characterization of somatic and germinal excision events lead us to conclude that excision of the wx-B3 Ac element almost always produces null alleles. Furthermore, the excellent correlation between the position of the wx-B3 mutation on the physical and genetic maps indicates that the Ac insertion is the only lesion of wx-B3. As a result, precise excision of this Ac should restore Wx function. The fact that revertant sectors and pollen grains are rare indicates that precise excision of Ac is also rare. The finding that the wx-B3 reversion frequency is comparable whether wx-B3 is hemizygous or over a wx allele with a wild-type insertion site illustrates a fundamental difference between the excision mechanisms of Ac and Drosophila P elements.

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