Ureaplasma Urealyticum and Male Infertility: An Animal Model*: II. Morphologic Changes of Testicular Tissue at Light Microscopic Level and Electron Microscopic Findings: Ureaplasma urealyticum und männliche Infertilität - ein Tiermodell: II. Morphologisch

Andrologia ◽  
2009 ◽  
Vol 21 (1) ◽  
pp. 66-75 ◽  
Author(s):  
H. Audring ◽  
H. Klug ◽  
R. Bollmann ◽  
W. Sokolowska-Köhler ◽  
S. Engel
Keyword(s):  
Animal Model ◽  
Male Infertility ◽  
Ureaplasma Urealyticum ◽  
Testicular Tissue ◽  
Microscopic Level ◽  
Männliche Infertilität ◽  
Light Microscopic Level ◽  
Electron Microscopic ◽  
Microscopic Findings ◽  
Morphologic Changes

Ureaplasma Urealyticum and Male Infertility: An Animal Model/Ureaplasma urealyticum und männliche Infertilität - ein Tiermodell

Andrologia ◽  
2009 ◽  
Vol 20 (6) ◽  
pp. 467-471 ◽  
Author(s):  
S. Engel ◽  
R. Bollmann ◽  
W. Sokolowska-Köhler ◽  
H. Audring ◽  
H. Klug
Keyword(s):  
Animal Model ◽  
Male Infertility ◽  
Ureaplasma Urealyticum ◽  
Männliche Infertilität

scholarly journals Immunogold probes for electron microscopy: evaluation of staining by fluorescence microscopy.

1985 ◽  
Vol 33 (10) ◽  
pp. 995-1000 ◽  
Author(s):  
R B Alexander ◽  
W B Isaacs ◽  
E R Barrack
Keyword(s):  
Electron Microscopy ◽  
Fluorescence Microscopy ◽  
Intermediate Filaments ◽  
Colloidal Gold ◽  
Microscopic Level ◽  
Secondary Antibody ◽  
Light Microscopic Level ◽  
Electron Microscopic ◽  
Cell Monolayers ◽  
Adenocarcinoma Cells

A method is presented whereby the staining of intracellular structures with immunogold probes for electron microscopy can be evaluated at the light microscopic level. Methanol-fixed monolayers of cultured Dunning R-3327-H rat prostatic adenocarcinoma cells were stained for cytokeratins using a two-step immunogold technique consisting of primary anti-keratin antibody followed by gold-labeled secondary antibody. Bound immunogold probe was then visualized with a fluorescent tertiary anti-immunogold probe antibody. Fluorescence microscopy of the whole cell monolayers showed a typical keratin cytoskeleton. The extra staining step did not interfere with subsequent fixation, embedding, and sectioning for electron microscopy, which showed cytoplasmic intermediate filaments decorated with colloidal gold. Using this method, it should be possible to manipulate parameters critical to staining with immunogold probes and to evaluate the labeling without necessitating repeated time-consuming electron microscopic processing. The method also provides a useful correlation between the light microscopic and ultrastructural labeling patterns of immunogold probes.

scholarly journals An approach to postembedding staining of protein (immunoglobulin) antigen embedded in plastic: prerequisites and limitations.

1980 ◽  
Vol 28 (10) ◽  
pp. 1041-1049 ◽  
Author(s):  
H Takamiya ◽  
S Batsford ◽  
A Vogt
Keyword(s):  
Biopsy Specimen ◽  
Picric Acid ◽  
Renal Tissue ◽  
Microscopic Level ◽  
Tissue Structure ◽  
Electron Microscopic Level ◽  
Light Microscopic Level ◽  
Electron Microscopic ◽  
Specific Localization ◽  
Ultrathin Sections

A method is described for performing postembedding staining of protein (immunoglobulin) antigen embedded in styrene-methacrylate resin. Fixation of specimens in a combination of 4% paraformaldehyde and 0.2% picric acid and washing in buffer containing 7% sucrose, followed by abrupt dehydration with absolute acetone in the cold preserved the antigenicity, although in a masked form. The masked antigenicity could be reexposed by treatment with nonspecific protease. Staining with fluorescent-, peroxidase-, or ferritin-labeled antibodies on semi- and ultrathin sections resulted in specific localization of the antigen. We applied this technique to the localization of rabbit immunoglobulin in specimens of renal tissue obtained from rats with anti-glomerular basement membrane nephritis; we also localized human IgG in a renal biopsy specimen. The prerequisites for recovery of antigenicity are such that preservation of tissue structure at the light microscopic level is good, but relatively poor at the electron microscopic level.

Natural Ostiotomy Vs. Inferior Antrostomy in the Management of Sinusitis: An Animal Model

1993 ◽  
Vol 109 (6) ◽  
pp. 1034-1042 ◽  
Author(s):  
Ilsa Schwartz ◽  
Michael S. Benninger ◽  
Janet Kaczor ◽  
Chad Stone
Keyword(s):  
Animal Model ◽  
Surgical Treatment ◽  
Maxillary Sinus ◽  
Chronic Inflammation ◽  
Chronic Sinusitis ◽  
Electron Microscopic ◽  
Microscopic Findings

An animal model was used to compare the surgical treatment of chronic sinusitis by opening the natural ostium vs. inferior antrostomy. Fifteen rabbits had surgical occlusion of the natural maxillary sinus ostia to induce sinusitis. At a second operation, the sinuses were entered and In each animal the natural ostia was reopened on one side and an Inferior antrostomy was performed on the opposite sinus. Eight weeks after these operations the sinuses were evaluated. No differences were found in antrostomy patency rates, gross evidence of acute or chronic inflammation, or light or electron microscopic findings between sinuses with natural ostiotomy and those with lower antrostomy. The gross appearances and light and electron microscopic findings are discussed.

scholarly journals Localization of HPV-16 DNA sequence in CaSki cells by electron microscopic hybridocytochemistry.

1992 ◽  
Vol 40 (4) ◽  
pp. 467-473 ◽  
Author(s):  
C T Lin ◽  
C C Chen ◽  
S W How ◽  
W M Huang ◽  
K Peck
Keyword(s):  
Electron Microscopy ◽  
Dna Sequence ◽  
Chromatin Structure ◽  
Carcinoma Cell ◽  
Microscopic Level ◽  
Light Microscopic Level ◽  
Electron Microscopic ◽  
Hpv 16 ◽  
Cervical Carcinoma Cell Line ◽  
Integrated Or

We investigated the HPV-16 DNA sequence in the CaSki cervical carcinoma cell line by electron microscopic hybridocytochemistry using biotinylated HPV-16/18 probes. At the light microscopic level, reaction product of hybridized HPV-16 DNA sequence was not seen in the cytoplasm but appeared as spots or rods randomly distributed in the nuclei. By electron microscopy, reaction product was seen aggregated in several regions in the nuclei. Most of the stained areas did not reveal particular architecture but showed part of the chromatin structure. In other nuclei, reaction product was observed to be associated with strings of loop-like structure, and some stained loops were seen to be connected directly to the nuclear filamentous chromatin structure. The skeletonized images of hybridized HPV-16 DNA in the nuclei were illustrated by computerized image analysis. In conclusion, we have demonstrated the HPV-16 DNA sequence in the nuclei of CaSki cells by electron microscopy. The identification of stained areas localized only in the chromatin suggests an integrated form of HPV-16 DNA sequence in the cells. This method could be used to identify an integrated or episomal form of viral DNA in the virus-containing cells.

The Production of Neuroaxonal Dystrophy in the Lateral Vestibular Nucleus of Rats by Centrifugation

1975 ◽  
Vol 33 ◽  
pp. 370-371
Author(s):  
J.E. Johnson
Keyword(s):  
Vestibular Nucleus ◽  
Vestibular Nuclei ◽  
Microscopic Level ◽  
Neuroaxonal Dystrophy ◽  
Lateral Vestibular Nucleus ◽  
Light Microscopic Level ◽  
Electron Microscopic ◽  
Gravitational Forces ◽  
The Central Nervous System ◽  
System Data

Very little is known about the effects of altered gravitational forces on the morphology of the central nervous system. Data so far published have been at the light microscopic level. The present abstract and corresponding article (in preparation) represent initial electron microscopic observations of changes in the lateral vestibular nuclei (VL) of rats centrifuged for varying lengths of time. Several groups of rats were employed in the study. Two groups were placed on a centrifuge (4.15 g's) at weaning and removed after 4 days. One group was conceived, born and raised on the centrifuge (4.15 g's) for 1 month. Other rats were placed on at weaning and left on for up to 21 months (2.76-4.l5 g's). All the centrifuged rats were sacrificed by aldehyde perfusion immediately after removal from the centrifuge.In the rat lateral vestibular nucleus, some synaptic terminals contain spherical (S) vesicles and others flattened (F) vesicles.

scholarly journals Photoconversion and electron microscopic localization of the fluorescent axon tracer fluoro-ruby (rhodamine-dextran-amine).

1993 ◽  
Vol 41 (5) ◽  
pp. 777-782 ◽  
Author(s):  
L C Schmued ◽  
L F Snavely
Keyword(s):  
Electron Microscopy ◽  
Green Light ◽  
Microscopic Level ◽  
Synaptic Connectivity ◽  
Electron Microscopic Level ◽  
Light Microscopic Level ◽  
Electron Microscopic ◽  
Axon Transport ◽  
Conventional Electron Microscopy ◽  
Electron Microscopic Localization

Fluoro-Ruby, the fluorescent tetramethylrhodamine-dextran-amine used to demonstrate anterograde axon transport, has been successfully photoconverted and subsequently localized by electron microscopy. The photoconversion was accomplished by irradiating the tissue with green light while bathing it in a solution containing DAB. The tissue could then be examined by brightfield microscopy or processed for conventional electron microscopy. Potential advantages of the technique include greater permanence and contrast at the light microscopic level and the ability to resolve synaptic connectivity at the electron microscopic level.

scholarly journals Mitochondrial associations with specific microtubular components of the cortex of Tetrahymena thermophila. I. Cortical patterning of mitochondria

1979 ◽  
Vol 39 (1) ◽  
pp. 299-312
Author(s):  
K. Aufderheide
Keyword(s):  
Tetrahymena Thermophila ◽  
Microscopic Level ◽  
The Other ◽  
Cell Cortex ◽  
Cortical Microtubules ◽  
Light Microscopic Level ◽  
Electron Microscopic ◽  
Cortical Patterning ◽  
A Cell ◽  
Nutrient Conditions

Many of the mitochondria of Tetrahymena thermophila are localized in the cell cortex in regular, identifiable patterns. Two different mitochondrial patterns are seen; whether a cell expresses one or the other type apparently depends upon nutrient conditions in the culture and not upon other factors tested. Consistent associations between cortical mitochondria and certain of the cortical microtubular bands are seen at the light-microscopic level. Electron-microscopic observations confirm this and, furthermore, identify ultrastructural associations between cortical microtubules and the cortically located mitochondria. It appears that the cortical microtubular arrays serve as a guide for the localization (and thus patterning) of the cortical mitochondria.

scholarly journals Immunocytochemical localization of renin in the submandibular gland of the mouse.

1978 ◽  
Vol 26 (10) ◽  
pp. 855-861 ◽  
Author(s):  
E Gresik ◽  
A Michelakis ◽  
T Barka ◽  
T Ross
Keyword(s):  
Submandibular Gland ◽  
Adult Mouse ◽  
Secretory Granules ◽  
Microscopic Level ◽  
Electron Microscopic Level ◽  
Light Microscopic Level ◽  
Electron Microscopic ◽  
Granular Convoluted Tubule ◽  
Enzyme Method ◽  
Unlabeled Antibody

Renin was localized in the submandibular gland of the adult mouse at light and electron microscopic levels by the unlabeled antibody enzyme method of Sternberger. At the light microscopic level, renin was confined to the granular convoluted tubule (GCT) segment of the gland with considerable variation among GCT cells in intensity of staining. Some GCT cells failed to stain for renin. The pattern of staining was the same in the gland of male and female mice, but in the glands of females GCT segments were smaller and less numerous. At the electron microscopic level, staining for renin was also confined to the GCT cells, and was localized exclusively to the secretory granules. The intensity of staining of the secretory granules within a given GCT cell varied; some cells contained only minimally reactive or negative secretory granules. All other organelles within the GCT cell, except condensing vacuoles, failed to stain.

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