Temperature tolerance, thermal inactivation and ultraviolet light resistance of nuclear polyhedrosis virus of the army worm, Mythimna separata (Walk) (Lepid.; Noctuidae)

2009 ◽  
Vol 87 (1-4) ◽  
pp. 82-90 ◽  
Author(s):  
D. Manjunath ◽  
S. B. Mathad
Keyword(s):  
Ultraviolet Light ◽  
Thermal Inactivation ◽  
Nuclear Polyhedrosis Virus ◽  
Temperature Tolerance ◽  
Mythimna Separata ◽  
Light Resistance ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis ◽  
Army Worm

Ultraviolet light activation of insect nuclear polyhedrosis virus

Nature ◽  
1975 ◽  
Vol 253 (5493) ◽  
pp. 628-629 ◽  
Author(s):  
W. A. RAMOSKA ◽  
GORDON R. STAIRS ◽  
W. FRED HINK
Keyword(s):  
Ultraviolet Light ◽  
Nuclear Polyhedrosis Virus ◽  
Light Activation ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

scholarly journals Pengujian Bahan Formulasi MsNPV ( Mythimna separata Nuclear Polyhedrosis Virus) terhadap Ulat Grayak Padi, Mythimna separata Walker di Lapangan

2016 ◽  
Vol 6 (1) ◽  
pp. 15
Author(s):  
Trisnaningsih Trisnaningsih ◽  
Arifin Kartohardjono
Keyword(s):  
Sea Level ◽  
Nuclear Polyhedrosis Virus ◽  
Block Design ◽  
Mythimna Separata ◽  
Life And Death ◽  
Randomized Block Design ◽  
Nuclear Polyhedrosis ◽  
And Control ◽  
Rice Plantation ◽  
Army Worm

The objective of these studies were to observe efficiency material formulations of MsNPV and the influence to rice plantation on different locations (Indramayu (3 m above sea level), Sukabumi (400m above sea level) and Bogor (250 m above sea level) to larvae of rice army worm. This study used randomized block design with 4 treatments consisted of 3 formulation materials (talc, kaolin and gypsum) and control in five replications. Observations were done to life and death larvae on 5, 10, 15 days after inoculation and leaf damaged. Data were analysed with DMRT. Results from this study indicated that material talc formulation was more effective because all the material was dissolve as compare to material kaolin and gypsum formulations while the material were precipitated. Utilize these three material formulations on three different locations above sea level gave the same impact to the mortality army worm larvae and also to the plant damage.

Nuclear Polyhedrosis Virus of the Gypsy Moth (Lepidoptera: Lymantriidae): Potency and Effects of Selected Adjuvants on Insect Feeding Behavior2

1995 ◽  
Vol 30 (4) ◽  
pp. 417-428 ◽  
Author(s):  
Robert R. Farrar ◽  
Richard L. Ridgway ◽  
Stephen P. Cook ◽  
Kevin W. Thorpe ◽  
Ralph E. Webb
Keyword(s):  
Feeding Behavior ◽  
Ultraviolet Light ◽  
Gypsy Moth ◽  
Nuclear Polyhedrosis Virus ◽  
Forest Service ◽  
Detectable Effect ◽  
Larval Feeding ◽  
Wettable Powder ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

The potency of two formulations of the nuclear polyhedrosis virus of the gypsy moth (LdMNPV) was evaluated in the laboratory. Both formulations were prepared with the same batch of LdMNPV produced in live insects by the USDA Forest Service. A Forest Service-recommended tank-mix preparation (LdMNPV, molasses, ultraviolet light screen, and sticker in water) was found to be about 20 times more potent than an experimental wettable powder preparation. The wettable powder also deterred feeding; the 20-fold difference in potency, though, is based on actual doses consumed. The addition of a stilbenedisulfonic acid derivative, Blankophor BBH, to the tank-mix and wettable powder preparations at a concentration of 1% (wt./vol.) reduced the LD50s by 42- and 214-fold, respectively. Blankophor BBH was also a moderate feeding deterrent to gypsy moth, Lymantria dispar (L.), larvae which could limit its efficacy as an enhancer of the virus. However, the addition of molasses to Blankophor BBH at least partially overcame the feeding deterrence. Other adjuvants were also tested for effects on larval feeding behavior, including Bond (sticker), Lignosite AN (ultraviolet light screen), and Carrier 244 (spray carrier). Of the materials tested, molasses was the strongest feeding stimulant, followed by Carrier 244. Bond and Lignosite AN had no detectable effect on feeding behavior in these tests. These data demonstrate the importance of monitoring potency during any formulation process, the possibility of enhancing the virus with adjuvants, and of understanding the effects of adjuvants on feeding behavior.

THE PERSISTENCE OF A NUCLEAR POLYHEDROSIS VIRUS IN THE HABITAT OF THE HOST INSECT, TRICHOPLUSIA NI: I. POLYHEDRA DEPOSITED ON FOLIAGE

1967 ◽  
Vol 99 (8) ◽  
pp. 785-794 ◽  
Author(s):  
R. P. Jaques
Keyword(s):  
Ultraviolet Light ◽  
Trichoplusia Ni ◽  
Laboratory Tests ◽  
Nuclear Polyhedrosis Virus ◽  
Cabbage Looper ◽  
Host Insect ◽  
Aqueous Suspensions ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

AbstractField, glasshouse, and laboratory tests showed that deposits of polyhedra of the nuclear polyhedrosis virus of the cabbage looper, Trichoplusia ni (Hübner), on foliage are practically non-infective after a 1-month exposure to weathering. Inactivation by sunlight appeared to be more important than removal by washing by rain in causing this loss of activity. The virus was readily inactivated by exposure to ultraviolet light. Virus exposed in aqueous suspensions of polyhedra or in wetted deposits was inactivated by shorter exposures than was virus in dry deposits of polyhedra.

Laboratory Evaluation of Selected Spray Adjuvants as Ultraviolet Light Protectants for the Nuclear Polyhedrosis Virus of the Celery Looper (Lepidoptera: Noctuidae)2

2000 ◽  
Vol 35 (3) ◽  
pp. 239-250 ◽  
Author(s):  
Robert R. Farrar ◽  
Richard L. Ridgway
Keyword(s):  
Ultraviolet Light ◽  
Nuclear Polyhedrosis Virus ◽  
Beet Armyworm ◽  
Laboratory Evaluation ◽  
Disulfonic Acid ◽  
Feeding Stimulant ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis ◽  
The Cost ◽  
Spray Adjuvants

Four spray adjuvants were tested in the laboratory as ultraviolet light (UV) protectants for the nuclear polyhedrosis virus of the celery looper, Anagraphs falcifera (Kirby) (AfMNPV), against the beet armyworm, Spodoptera exigua (Hübner). We tested a sodium lignin sulfonate (Lignosite AN®), two diaminostilbene disulfonic acid-derived fluorescent brighteners (Blankophor BBH® and Blankophor HRS®), and a nutrient-based feeding stimulant (Coax®). Lignosite AN was active as a UV protectant; Blankophor BBH, as both an enhancer and a UV protectant; Blankophor HRS, as an enhancer only, and; Coax, as a UV protectant only. Lack of an effect of Coax as a feeding stimulant may be due to the design of the bioassay, in which larvae were confined on small pieces of foliage. However, the practical utility of some, if not all, of these materials may be limited by the cost and/or bulk of the amounts required to achieve the desired effects.

scholarly journals EFEK PENYIMPANAN TERHADAP VIRULENSI NUCLEAR POLYHEDROSIS VIRUS YANG DIAPLIKASIKAN PADA TANAMAN KEDELAI TERSERANG ULAT GRAYAK (Spodoptera litura Fabr. )

2001 ◽  
Vol 1 (1) ◽  
pp. 29-32
Author(s):  
Sudi Pramono
Keyword(s):  
Spodoptera Litura ◽  
Field Experiments ◽  
Nuclear Polyhedrosis Virus ◽  
Block Design ◽  
Control Techniques ◽  
Polyhedrosis Virus ◽  
One Year ◽  
Nuclear Polyhedrosis ◽  
And Control ◽  
Army Worm

One of the problem increased soybean production was Spodoptera litura (army worm), because army worm has been known to be resistant to many insec¬ticides.  Alternatif control techniques should be explored  to reduce population of the pest.  Nuclear Polyhedrosis Virus (NPV) could storage one or more year,  but must used technique cold storage so that not virulence decreased.  This experiment was conducted to study (1) NPV patho¬genicity to army worm in the field, and (2) the effect of storage on the pathogenicity of NPV to S.  litura.  The research consisted of two experiments i.e.  laboratory and field experiments.  Each experiment had  seven treatments arranged in a randomized completely block design.  Each treatment was replicated four times.  The results show  the highest mortality of S.  litura occurred in plot treated  with 1,0 x 107 PIBs /ml, the mortality was 48 to 49,33 spec/plot after ten days aplicated .  If comparative patogenicity the new and the old NPV was not significant.  Population of army worm decreased significantly by NPV and control.  So that patogenecity NPV storage  one  year as well as new NPV  against army worm.     

The inactivation of the nuclear polyhedrosis virus of Trichoplusia ni by gamma and ultraviolet radiation

1968 ◽  
Vol 14 (10) ◽  
pp. 1161-1163 ◽  
Author(s):  
R. P. Jaques
Keyword(s):  
Ultraviolet Radiation ◽  
Gamma Radiation ◽  
Ultraviolet Light ◽  
Trichoplusia Ni ◽  
Nuclear Polyhedrosis Virus ◽  
Aqueous Suspension ◽  
Aqueous Suspensions ◽  
Bacterial Contaminants ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

The activity of the nuclear polyhedrosis virus of Trichoplusia ni was reduced by 90% when exposed as an aqueous suspension of polyhedra to a dose of 3 × 105 rads of gamma radiation. A dose in excess of 1 × 107 rads completely inactivated the virus in suspensions. The virus was more readily inactivated when exposed as dry deposits of polyhedra than as aqueous suspensions. Bacterial contaminants in the viral suspensions were more sensitive to gamma radiation than was the virus but the virus was the more sensitive to ultraviolet light.

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