scholarly journals Pengujian Bahan Formulasi MsNPV ( Mythimna separata Nuclear Polyhedrosis Virus) terhadap Ulat Grayak Padi, Mythimna separata Walker di Lapangan

2016 ◽  
Vol 6 (1) ◽  
pp. 15
Author(s):  
Trisnaningsih Trisnaningsih ◽  
Arifin Kartohardjono

The objective of these studies were to observe efficiency material formulations of MsNPV and the influence to rice plantation on different locations (Indramayu (3 m above sea level), Sukabumi (400m above sea level) and Bogor (250 m above sea level) to larvae of rice army worm. This study used randomized block design with 4 treatments consisted of 3 formulation materials (talc, kaolin and gypsum) and control in five replications. Observations were done to life and death larvae on 5, 10, 15 days after inoculation and leaf damaged. Data were analysed with DMRT. Results from this study indicated that material talc formulation was more effective because all the material was dissolve as compare to material kaolin and gypsum formulations while the material were precipitated. Utilize these three material formulations on three different locations above sea level gave the same impact to the mortality army worm larvae and also to the plant damage.

2001 ◽  
Vol 1 (1) ◽  
pp. 29-32
Author(s):  
Sudi Pramono

One of the problem increased soybean production was Spodoptera litura (army worm), because army worm has been known to be resistant to many insec¬ticides.  Alternatif control techniques should be explored  to reduce population of the pest.  Nuclear Polyhedrosis Virus (NPV) could storage one or more year,  but must used technique cold storage so that not virulence decreased.  This experiment was conducted to study (1) NPV patho¬genicity to army worm in the field, and (2) the effect of storage on the pathogenicity of NPV to S.  litura.  The research consisted of two experiments i.e.  laboratory and field experiments.  Each experiment had  seven treatments arranged in a randomized completely block design.  Each treatment was replicated four times.  The results show  the highest mortality of S.  litura occurred in plot treated  with 1,0 x 107 PIBs /ml, the mortality was 48 to 49,33 spec/plot after ten days aplicated .  If comparative patogenicity the new and the old NPV was not significant.  Population of army worm decreased significantly by NPV and control.  So that patogenecity NPV storage  one  year as well as new NPV  against army worm.     


2018 ◽  
Vol 35 (1) ◽  
pp. 143-150
Author(s):  
S. Sharma ◽  
G. S. Bhandari ◽  
S. Neupane ◽  
A. Pathak ◽  
S. Tiwari

The armyworm, Mythimna separata Walker is the potential insect pest of maize, causes a severe damage on the vegetative and reproductive stages of that plant. Insecticides are the common practices to manage the broad categories of the pest in a maize field. Further, these practices have been linked to farmers health, biodiversity loss and declining of predatory arthropods. However, pest management by adopting a tool of integrated pest management is one potential option to reduce the pesticide in maize fields. Field experiments were conducted in winter maize with the aim of evaluating the potential bio-rational pesticides to manage the armyworm. The studies were conducted for two consecutive years in 2015/16 and 2016/17 at Rampur, Chitwan. Treatments were: Metarrhizium anisopliae (0.2ml/lit water), Nuclear Polyhedrosis Virus (NPV) ( 2.5gm/lit water), Spinosad 45% SC (0.25ml/liter of water), Multineem (Azadriachta indica) (2 .0 ml/ liter water), Furadan 3G (3-4g/ Whorl application), Lara (Chloropyriphos 50 EC + Cypermethrin 5 EC) (1.0 ml/liter of water), Magik (Imidacloprid 17.8% (0.5ml/liter of water) and Control. The experiments were completely randomized block design with three replicates. Spinosad treated plots demonstrated the lowest damage (0.884%) followed by Magik (1.27%) which was significantly lower (P< 0.05) than other treatments in 2015/2016. However, in 2016/2017, Spinosad treated plot also showed the significantly lowest damage (1.67%) followed by Lara plots (2.67%), both of these were significantly different (P< 0.05) to Metarhizium and control plots, but similar to the other treatments, but not significantly so. Maximum yield was recorded in spinosad treated plots (9.8 t/ha and 9.12 t/ha) followed by Lara treated plots (9.17t/ha and 8.69t/ha) and were lowest in control plots (4.55 t/ha and 7.18 t/ha) for both years. Hence, Spinosad (soil actinomycete bacterium, Saccharopolyspora spinose ) has the potentiality to maximize the maize yield and also potentially to the reduce the pesticide consumption and incurred cost caused by the pesticide use. Such a safe and eco-friendly insecticide has the potential to replace the synthetic pesticide and provide the benign environment to the natural predators and service providing units of multiple ecosystem services in maize agro-ecosystem.


2015 ◽  
Vol 2 (1) ◽  
pp. 649
Author(s):  
Anasthasia Vehuliza Surbakti ◽  
Mia Miranti ◽  
Melanie .

<p>The biologicaltest of formulation of subculture Helicoverpa armigera Nuclear Polyhedrosis Virus(HaNPV) against Crocidolomia pavonana Fab.larvae population that exposed to cabbage (Brassica oleracea var. capitata L.) has been done. The subculture HaNPV was formulated in liquid preparations, powders, cornstarch and talc carrier materials, sprayed to C. pavonana larvae population that exposed at cabbage as a pilot project. The method of research used an experimental method, with randomized block design consists of single factor HaNPV and five level formulation there were (p) (liquid dosage forms (p1), powder (p2), and mixed with a carrier such as cornstarch (p3) and talc powder (p4) as well as the provision of water control without virus (p0)) with 8 replications. The density of virus 4x107 polyhedral/ml. The results was statistically analyzed with ANOVA (p&lt;0,05), showed that all formulation HaNPV subculture have equal ability to caused high mortality of C. pavonana larvae population. <br /><strong></strong></p><p><strong>Keywords</strong>: Cabbage, Crocidolomia pavonana, Formulation,HaNPV, Mortality</p>


Author(s):  
Ikhsan Gatot Aji Prasetio ◽  
Wawan Hermawan ◽  
Mia Miranti ◽  
Camellia Panatarani ◽  
I Made Joni ◽  
...  

The constraints on the effectiveness of nuclear polyhedrosis virus (NPV) as biocontrol are usually due to environmental factors such as temperature and ultraviolet (UV) exposure. Zeolite has been commonly used as a carrier or delivery system for nuclear polyhedrosis viruses. In this study, zeolite powder was reduced into nanosized particles by beads milling method and was investigated for the effect of its concentration in the delivery system of Helicoverpa armigera nuclear polyhedrosis virus (HaNPV1) on the lethal time against the larvae Crocidolomia pavonana. The formulation used three concentrations of nanozeolite suspension, 0.5, 1, 1.5, and 2 wt.% applied for each 4 × 107 of HaNPV1. A randomized block design (RBD) method was applied with 3 replications. The results showed that the scanning electron microscope (SEM) from nanozeolite was seen coating the entire surface of the HaNPV1 polyhedra and an increase of zeolite concentration caused acceleration of the lethal time of C. pavonana instar III. Thus, the fastest lethal time was 1.2 days receiving a concentration of 2 wt.%, which was significantly higher compared to without delivery (2.9 days). The increase of the zeolite concentration up to 2 wt.% in the delivery system for HaNPV1 improved their performance on lethal time and mortality against C. pavonana. It was concluded that nanozeolite as a delivery system enhanced and created a synergy in infecting C. pavonana.


Author(s):  
Nihad H. Mutlag ◽  
Ameer S. A. Al-Haddad

A field study was conducted to evaluate the efficiency of four microbial insecticides viz. Beauveria bassiana; HaNPV (Helicoverpa armigera Nuclear Polyhedrosis virus); (Bacillus thuringiensisvar.kurstaki 2 gm/L); HaNPV+Bt; neem oil; neem cake and D.D.V.P EC 76% @0.05% at Research Farm SHIATS,Allahabad during rabi season of 2011-2012. The experiment was laid out in randomized block design with seven treatment and replicated thrice. The observation larval populations of H. armigera were recorded one day before treatment was recorded at 3,7, and 10 days after treatments. The larva population of H. armigera appeared in the third week of February (8 the Standard week) and reached its peak of 14.65 larvae in first week of April and decline rapidly with maturation of crop. There was only one peak in the larval population observation in the 1st week. Bacillus thuringiensis was the most effective chemical by D.D.V.P.76%@0.05% . Among the microbial insecticides. HaNPV ,was the most effective followed by HaNPV+Bt and neem cake . The combination treatments were less effective than the individual treatment neem oil and B. bassiana were the least effective treatment in reducing the larval population of Heliverpa armigera.


Author(s):  
Lia Amalia ◽  
Ai Komariah ◽  
Ilma Hilmayanti

Experiments to study the stability and adaptability genotype of hybrid rice were conducted in two locations in West Java: 1. the Jelekong Village Baleendah Bandung region, 2. the Arjasari Village Leuwisari Tasikmalaya region. The altitude of the Baleendah Bandung region is 650 and that of the Leuwisari Tasikmalaya region is 450 meters above sea level. The experiments were arranged in a randomized block design and used with 9 genotypes and 3 replications. The 9 genotypes of hybrid rice are SW-907, SW-804, SW-902, US-915, SW-82, SW-923, Intani-2, SL 8 SHS, and SHS 04 WM. The result of the experiment showed that the whole genotypes A = SW-907, B = SW-804, C = SW-902, D = US-915, E = SW-82, F = SW- 923, G = WM 4 SHS, H = SL 8 SHS, and I = Intani 2 measured the height of the plants, the number of productive tillers, the length of the panicles, the number of grains per panicle, the number of filled grains per panicle, the weight of 1000 grains, and the grain yield per plot, and were unstable and unadaptive in Bandung and Tasikmalaya.


2018 ◽  
Vol 10 (2) ◽  
pp. 152-158
Author(s):  
Hari Yani Fadillah

The research aims to know the effect of tomato juice on blood pressure in pre-elderly pre hypertension at Posbindu Puskesmas Nusa Indah Bengkulu City.The design of research was a quasi experiment include pre test post test. This population was all patients posbindu aged 45-59 years. Sampling of this research randomized block design of one factorial were 6 samples divided into treatment and control group. The data which have been analyzed using univariate and bivariate. From this research were obtained the results such as the mean of sistole blood pressure before and after intervention in treatment group p = 0,273 and diastole p = 0,27. Mean systole blood pressure control group p = 0.060 and diastole p = 2.14. Distribution of tomato juice (Lycopersicum grandifilum) did not given affect the decrease in blood pressure of systole diastole before and after intervention in pre-elderly pre hypertension. Whereas the difference in mean systole of blood pressure treatment and control group p = 0.935 and diastolic of blood pressure p = 0.678. There ware not difference in mean of blood pressure in the tomato juice treatment group and control of Vitamin C. The result concluded there were not effect of tomato juice (Lycopersicum grandifilum) on systolic of blood pressure in pre-elderly pre hypertension at Posbindu Puskesmas Nusa Indah Bengkulu City.


2017 ◽  
Vol 4 (1) ◽  
pp. 59
Author(s):  
Yudi Yusdian ◽  
Ridwan Haris

The objective of this research was to study the response on the growth of clove seedling cultivar Zanzibar as the result of using  NPK Phonska fertilizer dosage and liquid organic fertilizer  concentration and to find out the acurate dosage of  NPK Phonska and liquid organic fertilizer  “Getoe”. The experiment was conducted at Kampung Ciherang Desa Gunungtua Kecamatan Cijambe Kabupaten Subang, West Java. The altitude is about 600 m above sea level, the soil type is Ultisol (pH 5,4), the average of rainfall was 1622,10 mm/year and the type of rainfall is C according to Schmidt and Ferrguson (1951). The experiment from March 2014 until June 2014. The experiment was arranged in Randomized Block Design (RBD) consisted of  six treatments, i.e :  A (2,5 g NPK + 0 cc/L of water POC Getoe), B (1,5 g NPK + 2,5 cc/L of water POC Getoe), C (2,0 g NPK + 2 cc/L of water POC Getoe), D (2,5 g NPK + 1,5 cc/L of water POC Getoe), E (3,0 g NPK + 1,0 cc/L of water POC Getoe) and F (3,5 g NPK + 0,5 cc/L of water POC Getoe) and replicated six times respectively. The result of this research showed that combined 2,5 g NPK fertilizer and 1,5 cc Getoe liquid organic fertilizer  concentration  gave the better effect on stem height, leaves of number, leaf area, dry weight of plant and dry weight of root the clove seedling cultivar Zanzibar. 


2018 ◽  
Vol 35 (1) ◽  
pp. 203-209
Author(s):  
M. Basnet ◽  
H. Gurung

An experiment was conducted at Salyan, Nepal to assess the mother rhizome retrieval and organic treatment of the same retrieved plant on economic yield of ginger during 2015/16.‘ Kapurkot Aduwa-1’, a superior Nepalese variety was used for this experiment. Two dates i.e. 1 September and 7 September were used as a time of retrieval factor and seven organic treatments i.e. Trichoderma, Jeevatu, Bordeaux paste, mixture of neem and timur dust, vermicompost, jholmal and control as second factor and these 14 treatment combinations were laid out in factorial randomized block design with three replications. The plant height, leaf number, tiller number was found to be significantly highest with vermicompost treatment on all days 5 days interval after harvesting of the mother rhizome whereas the lowest was observed at control. The mother rhizome yield and fresh rhizome was found to be significantly highest on vermicompost applied field with 1.7 t/ha and 22.8 mt/ha respectively and lowest fresh rhizome yield (15.5. t/ha) was found with control. The disease yield was significantly lowest (0 t/ha) and highest (0.2 t/ha) with the control. Benefit cost ratio was found to be significantly highest (1.74) and lowest at control (1.32).


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