scholarly journals EFEK PENYIMPANAN TERHADAP VIRULENSI NUCLEAR POLYHEDROSIS VIRUS YANG DIAPLIKASIKAN PADA TANAMAN KEDELAI TERSERANG ULAT GRAYAK (Spodoptera litura Fabr. )

2001 ◽  
Vol 1 (1) ◽  
pp. 29-32
Author(s):  
Sudi Pramono

One of the problem increased soybean production was Spodoptera litura (army worm), because army worm has been known to be resistant to many insec¬ticides.  Alternatif control techniques should be explored  to reduce population of the pest.  Nuclear Polyhedrosis Virus (NPV) could storage one or more year,  but must used technique cold storage so that not virulence decreased.  This experiment was conducted to study (1) NPV patho¬genicity to army worm in the field, and (2) the effect of storage on the pathogenicity of NPV to S.  litura.  The research consisted of two experiments i.e.  laboratory and field experiments.  Each experiment had  seven treatments arranged in a randomized completely block design.  Each treatment was replicated four times.  The results show  the highest mortality of S.  litura occurred in plot treated  with 1,0 x 107 PIBs /ml, the mortality was 48 to 49,33 spec/plot after ten days aplicated .  If comparative patogenicity the new and the old NPV was not significant.  Population of army worm decreased significantly by NPV and control.  So that patogenecity NPV storage  one  year as well as new NPV  against army worm.     

2016 ◽  
Vol 6 (1) ◽  
pp. 15
Author(s):  
Trisnaningsih Trisnaningsih ◽  
Arifin Kartohardjono

The objective of these studies were to observe efficiency material formulations of MsNPV and the influence to rice plantation on different locations (Indramayu (3 m above sea level), Sukabumi (400m above sea level) and Bogor (250 m above sea level) to larvae of rice army worm. This study used randomized block design with 4 treatments consisted of 3 formulation materials (talc, kaolin and gypsum) and control in five replications. Observations were done to life and death larvae on 5, 10, 15 days after inoculation and leaf damaged. Data were analysed with DMRT. Results from this study indicated that material talc formulation was more effective because all the material was dissolve as compare to material kaolin and gypsum formulations while the material were precipitated. Utilize these three material formulations on three different locations above sea level gave the same impact to the mortality army worm larvae and also to the plant damage.


2015 ◽  
Vol 2 (1) ◽  
pp. 646
Author(s):  
Desy Efriyani Anggraeny Nasution ◽  
Mia Miranti ◽  
Melanie .

<p>Helicoverpa armigera Nuclear Polyhedrosis Virus (HaNPV) is one of the biological agents that could be used for the development of organic farming in Indonesia. One of the organic agricultural food products which have high economic value is cabbage, HaNPV effectif to control the population of several species of insect pests, can be used instead of synthetic insecticides in agricultural development efforts of organic cabbage. HaNPV formulation can be used as a way to maintain the virulence properties of the virus and facilitate the storage and application. The preparation of subculture HaNPV formulations was in liquid, powders, cornstarch and talk carrier material. The design experimental was used randomized block design consists single factor (type HaNPV formulation consisting of powder formulations, liquid formulation, formulation in cornstarch, and talc formulation including water formulation without virus as a control), with eight replications. The results showed that all of the formulations of subculture HaNPV causing larvae mortality at range 97.4% -100%. The highest mortality of larvae occurred in cabbage plants are protected by HaNPV carrier liquid and talk preparations by 100% each. <br /><strong></strong></p><p><strong>Keywords</strong>: Formulations Helicoverpa armigera Nuclear Polyhedrosis Virus (HaNPV), subculture, Spodoptera litura, Brassica oleracea Var. capitata Linn.</p>


2000 ◽  
Vol 132 (3) ◽  
pp. 337-340 ◽  
Author(s):  
M.D. Monobrullah ◽  
Masao Nagata

Susceptibility of many insects to baculoviruses decreases with host development (Hochberg 1991), whereas the time required for infected hosts to die increases with larval age (Boucias et al. 1980; Bucher and Turnock 1983; Smits and Vlak 1988). The actual mechanism of this phenomenon, however, has not been established in many cases. The present study was undertaken to investigate the effect of larval age on the susceptibility of Spodoptera litura Fabricius to nuclear polyhedrosis virus (NPV) through oral and intrahaemocoelic routes of infection.


2019 ◽  
Vol 51 (2) ◽  
Author(s):  
Sumaira Maqsood ◽  
Muhammad Afzal ◽  
Muhammad Saleem Haider ◽  
Hafiz Azhar Ali Khan ◽  
Muhammad Ali ◽  
...  

2019 ◽  
Vol 2 (2) ◽  
pp. 46
Author(s):  
M Novel Ghufron Syahroni ◽  
Nanang Tri Haryadi

Spodoptera litura (armyworm) is an important leaf pest that is polyphagic and has a broad range of hosts. Spodoptera litura is the main pest in soybean plants and can cause up to 50% damage and often results in decreased productivity and even crop failure. Spodoptera litura is widespread in areas with hot and humid climates from the subtropics to the tropics. S. litura pests attack cultivation plants in the vegetative and generative phases. The prospect of insect pathogens as a substitute for chemical insecticides is quite good in terms of their effectiveness and impact on the environment. Nuclear Polyhedrosis Virus (NPV) is one of the Baculoviridae viruses that infect the larval stage. Spodoptera litura Nuclear Polyhedrosis Virus (SlNPV) is a pathogenic virus that infects armyworms. SlNPV JTM 97C is an effective isolate in controlling larvae of Spodoptera litura. The larvae used in this study were larval instar 4, instar 5, and instar 6. The study was conducted using a Completely Randomized Design (CRD) with 6 treatments and 3 replications. The treatments consisted of controls, 1 g / l, 2g / l, 3 g / l, 4 g / l, and 5 g / l. Each treatment was applied to instar 4, instar 5 and 6 instar larvae. The results showed that SlNPV JTM 97C affected mortality, stopped eating and pupa formation of instar 4, instar 5 larvae, but did not affect instar 6 larvae. The younger the larval instar the higher the pathogenicity response.


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