Sperm motility and seminal fluid composition in the burbot, Lota lota

The role of Ca2+ in sturgeon sperm maturation and motility was investigated. Sperm from mature male sterlets (Acipenser ruthenus) were collected from the Wolffian duct and testis 24 h after hormone induction. Testicular spermatozoa (TS) were incubated in Wolffian duct seminal fluid (WDSF) for 5 min at 20°C and were designated ‘TS after IVM’ (TSM). Sperm motility was activated in media with different ion compositions, with motility parameters analysed from standard video microscopy records. To investigate the role of calcium transport in the IVM process, IVM was performed (5 min at 20°C) in the presence of 2 mM EGTA, 100 µM Verapamil or 100 µM Tetracaine. No motility was observed in the case of TS (10 mM Tris, 25 mM NaCl, 50 mM Sucr with or without the addition of 2 mM EGTA). Both incubation of TS in WDSF and supplementation of the activation medium with Ca2+ led to sperm motility. The minimal Ca2+ concentration required for motility activation of Wolffian duct spermatozoa, TS and TSM was determined (1–2 nM for Wolffian duct spermatozoa and TSM; approximately 0.6 mM for TS). Motility was obtained after the addition of verapamil to the incubation medium during IVM, whereas the addition of EGTA completely suppressed motility, implying Ca2+ involvement in sturgeon sperm maturation. Further studies into the roles of Ca2+ transport in sturgeon sperm maturation and motility are required.

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Correlation Between Ultrasonic Testicular Volume and Seminal Fluid Analysis in Men with Infertility

European Journal of Medical and Health Sciences ◽

10.24018/ejmed.2021.3.1.541 ◽

2021 ◽

Vol 3 (1) ◽

pp. 103-107

Author(s):

Sadiq Abu ◽

Hadijat O. Kolade-Yunusa ◽

Terkaa Atim ◽

F. Ehimatie Obakeye ◽

Nuhu K. Dakum

Keyword(s):

Sperm Motility ◽

Teaching Hospital ◽

Seminal Fluid ◽

Sperm Concentration ◽

Testicular Volume ◽

Seminiferous Tubules ◽

Testicular Function ◽

Semen Volume ◽

Significant Difference ◽

The Mean

Background: Infertility is a major cause of marital disharmony in Nigeria because of the high premium placed on childbearing. Unfortunately, the blame is on the woman most times in Nigeria. Seminiferous tubules comprise 80-90% of testicular mass. Thus, the testicular volume is an index of spermatogenesis. Therefore, accurate testicular volume will help in assessing testicular function if there is no obstruction. This study was conducted to evaluate the correlation between testicular volume, measured by ultrasound and conventional sperm parameters (semen volume, sperm concentration, sperm motility and sperm morphology) in men with infertility presenting to Urology Division, University of Abuja Teaching Hospital, Gwagwalada Abuja. Methodology: This was a descriptive cross sectional study, male subjects with infertility presenting to University of Abuja Teaching Hospital were recruited into the study. The testicular volume of all the subjects was measured by ultrasonography. The semen samples were collected by the process of masturbation on day five of sexual abstinence and analyzed according to WHO criteria 2010. Collated data were analyzed using SPSS version 20.0. P-value<0.05 was taken as statistically significant. Results: The average total testicular volume (TTV) of men with infertility was 19.83 ml while mean testicular volume (MTV) was 10.24 ml. There was a statistically significant difference between MTV and semen volume (r=0.391, p=0.000), and sperm concentration (r=0.639, p=0.000). There was a weak and insignificant correlation between MTV and sperm motility and MTV and morphology (r=0.216, p=0.055 and r=0.076, p=0.502) respectively. Linear regression analysis showed significant impact of MTV (P<0.001) on sperm concentration with a critical MTV of ≤10ml.0 indicating poor testicular function (severe oligospermia) Conclusion: The mean ultrasonic testicular volume in men with infertility in this study was 10.24ml and there was a significant positive correlation of MTV with semen volume and sperm concentration. Although, there was correlation of MTV with sperm motility and morphology, this was not significant. The critical mean ultrasonic testicular volume indicating poor testicular function (severe oligospermia) was found to be 10ml. This study has shown that the mean ultrasonic testicular volume can be a pointer to semen volume and sperm concentration in evaluating men with infertility.

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Hormonal induction of Brycon cephalus (Characiformes, Characidae) to spermiation using D-ala6, pro9net-mGnRH + metoclopramide

Zygote ◽

10.1017/s0967199415000210 ◽

2015 ◽

Vol 24 (3) ◽

pp. 319-325 ◽

Cited By ~ 3

Author(s):

Cristiane Bashiyo-Silva ◽

Raphael da Silva Costa ◽

Douglas de Castro Ribeiro ◽

José Augusto Senhorini ◽

Rosicleire Veríssimo-Silveira ◽

...

Keyword(s):

Single Dose ◽

Sperm Motility ◽

Significant Variation ◽

Seminal Fluid ◽

Physiological Solution ◽

Control Group ◽

Seminal Parameters ◽

Live Fish ◽

Hormonal Induction

SummaryThis study aimed to establish a hormonal induction protocol for spermiation of Brycon cephalus males, using Ala6, Pro9Net-mGnRH + metoclopramide (Ovopel®). Thus, 20 males were used divided into three inductor treatments [⅓ pellet/kg (T1), ⅔ pellet/kg (T2) and 1⅓ pellet/kg (T3)] and one control group (CO), which only received physiological solution applications (0.9% NaCl). All treatments were applied in a single dose. For evaluation of the availability of the treatment, the following seminal parameters were analyzed: seminal volume, subjective spermatic motility, duration of motility, pH, osmolality and spermatic concentration. T3 showed the highest seminal volume (4.66 ± 1.52 ml), and was significantly different in comparison with T1 (2.0 ± 0.9 ml), T2 (3.5 ± 1.3 ml) and CO (2.3 ± 1.2 ml). In relation to spermatic motility, T2 and T3 showed significantly higher levels [5, (81–100%)]. However, T3 showed significantly lower average sperm motility duration than T1, T2 and CO (30 ± 7 s; 28 ± 6 s; 32 ± 8 s, respectively). With regard to the seminal parameters of spermatic concentration, pH and osmolality, no significant variation was verified among treatments. In conclusion, mGnRH + metoclopramide used for hormonal induction of B. cephalus reproduction does not induce changes related to spermatic concentration, pH and osmolality parameters of the seminal fluid and the most adequate doses among tested treatments were ⅔ pellet/kg live fish.

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Social manipulation of sperm competition intensity reduces seminal fluid gene expression

Biology Letters ◽

10.1098/rsbl.2017.0659 ◽

2018 ◽

Vol 14 (1) ◽

pp. 20170659 ◽

Cited By ~ 20

Author(s):

Nadia S. Sloan ◽

Maxine Lovegrove ◽

Leigh W. Simmons

Keyword(s):

Gene Expression ◽

Sperm Competition ◽

Seminal Fluid ◽

Fertilization Success ◽

Accessory Gland ◽

Fluid Composition ◽

Competition Intensity ◽

Teleogryllus Oceanicus ◽

Sperm Competition Risk ◽

Considerable Body

A considerable body of evidence supports the prediction that males should increase their expenditure on the ejaculate in response to sperm competition risk. The prediction that they should reduce their expenditure with increasing sperm competition intensity is less well supported. Moreover, most studies have documented plasticity in sperm numbers. Here we show that male crickets Teleogryllus oceanicus exhibit reduced seminal fluid gene expression and accessory gland mass in response to elevated sperm competition intensity. Together with previous research, our findings suggest that strategic adjustments in seminal fluid composition contribute to competitive fertilization success in this species.

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TITRATIONS OF HUMAN SEMINAL FLUID WITH ACIDS AND ALKALIS AND THEIR EFFECTS ON THE SURVIVAL OF SPERM MOTILITY

American Journal of Physiology-Legacy Content ◽

10.1152/ajplegacy.1942.136.3.535 ◽

1942 ◽

Vol 136 (3) ◽

pp. 535-541 ◽

Cited By ~ 17

Author(s):

Leo Shedlovsky ◽

Donald Belcher ◽

Irving Levenstein

Keyword(s):

Sperm Motility ◽

Seminal Fluid

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Correlation Between Sperm Motility and Electrolyte Composition of Seminal Fluid in Normal and Infertile Men

Fertility and Sterility ◽

10.1016/s0015-0282(16)39498-5 ◽

1973 ◽

Vol 24 (2) ◽

pp. 150-154 ◽

Cited By ~ 15

Author(s):

Augusto Bondani ◽

Emilia Aspeitia ◽

Ramón Aznar ◽

Ernesto Gómez-Arzápalo ◽

Carlos Pascual ◽

...

Keyword(s):

Sperm Motility ◽

Seminal Fluid ◽

Electrolyte Composition ◽

Infertile Men

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Sperm competition risk drives plasticity in seminal fluid composition

BMC Biology ◽

10.1186/s12915-015-0197-2 ◽

2015 ◽

Vol 13 (1) ◽

Cited By ~ 49

Author(s):

Steven A. Ramm ◽

Dominic A. Edward ◽

Amy J. Claydon ◽

Dean E. Hammond ◽

Philip Brownridge ◽

...

Keyword(s):

Sperm Competition ◽

Seminal Fluid ◽

Fluid Composition ◽

Sperm Competition Risk

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Exposure to sperm competition risk improves survival of virgin males

Biology Letters ◽

10.1098/rsbl.2012.1188 ◽

2013 ◽

Vol 9 (2) ◽

pp. 20121188 ◽

Cited By ~ 6

Author(s):

Joshua P. Moatt ◽

Calvin Dytham ◽

Michael D. F. Thom

Keyword(s):

Sperm Competition ◽

Life History Traits ◽

Seminal Fluid ◽

Fluid Composition ◽

Short Term ◽

Investment In Reproduction ◽

Sperm Competition Risk ◽

Total Investment ◽

Improvement In Survival ◽

Striking Effect

Sperm competition between the ejaculates of multiple males for the fertilization of a given set of ova is taxonomically widespread. Males have evolved remarkable adaptations to increase their reproductive success under postcopulatory sexual selection, which in many species includes the ability to modify behaviour and ejaculate characteristics plastically to match the perceived level of sperm competition. Males of the model species Drosophila melanogaster increase mating duration and modify seminal fluid composition in response to short-term changes in sperm competition risk. If these responses increase a male's total investment in reproduction, he must either trade-off this cost against other life-history traits or suffer reduced survival. We tested whether mounting a plastic sperm competition response bears an instantaneous survival cost, and instead found that male D. melanogaster exposed to a high risk of sperm competition survive 12 per cent longer than those at low risk, equating to a 49 per cent reduction in the hourly hazard of death. This striking effect was found only among virgins: the high cost of mating in this species eliminates any such benefit among non-virgin males. Our results suggest that the improvement in survival found among virgins may be a product of males’ tactical responses to sperm competition.

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