Correlation Between Sperm Motility and Electrolyte Composition of Seminal Fluid in Normal and Infertile Men

1973 ◽  
Vol 24 (2) ◽  
pp. 150-154 ◽  
Author(s):  
Augusto Bondani ◽  
Emilia Aspeitia ◽  
Ramón Aznar ◽  
Ernesto Gómez-Arzápalo ◽  
Carlos Pascual ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Seminal Fluid ◽  
Electrolyte Composition ◽  
Infertile Men

scholarly journals Beneficial Role of Platelet –Rich Plasma (PRP) combined with Glass Wool Filtration for Best Recovery of Spermatozoa in Infertile Men

2021 ◽  
Vol 2 (5) ◽  
pp. 11-13
Author(s):  
Watfaa A. Abduljabar ◽  
Hayder A. L. Mossa ◽  
Muayad S. Abood
Keyword(s):  
Sperm Motility ◽  
Assisted Reproductive Technologies ◽  
Seminal Fluid ◽  
Platelet Rich Plasma ◽  
Reproductive Technologies ◽  
Glass Wool ◽  
Activation Technique ◽  
Fluid Analysis ◽  
Infertile Men ◽  
Before And After

Background: Platelet –Rich Plasma (PRP) is a novel therapeutic agent used in multiple medical fields and one of these fields is the reproduction for best spermatozoa preparation and activation for upgrading activity and motility of the spermatozoa and filtered with glass wool filtration to eliminate any round cells and leukocytes from the sample. Objectives: The aim of this research is to study some sperm characteristics in two groups, the normozoospermic infertile men and th asthenozoospermic infertile group before and after activation with Glass wool Filtration and PRP and compare between both. Patients and Methods: In this study 60 infertile men were enrolled and divided into 2 groups,15 normozoospermic infertile men, and 45 ashenozoospermic infertile men during their attendance to the Infertility Clinic in High Institute for Infertility Diagnosis and Assisted Reproductive Technologies Al-Nahrain University. The collected semen samples, and seminal fluid analysis were assessed, each semen sample was divided into 3 tubes and labelled as the 1st tube which was before activation, the 2nd tube was after glass wool filtration activation technique and the 3rd tube was for semen activated by adding PRP to the medium used for glass wool filtration activation. Results: Both techniques showed increased and improved sperm motility, but the PRP was superior to the glass wool alone in upgrading Sperm Grade A Motility %, Sperm Grade B Motility % and decreasing Sperm Grade C Motility %, and Sperm Grade D Motility (Immotile Sperm). Conclusion: The PRP was significantly effective in improving the sperm activity and upgrading sperm motility more than Glass Wool Filtration technique.

Role of Ca2+ in the IVM of spermatozoa from the sterlet Acipenser ruthenus

2017 ◽  
Vol 29 (7) ◽  
pp. 1319 ◽  
Author(s):  
Olga Bondarenko ◽  
Borys Dzyuba ◽  
Marek Rodina ◽  
Jacky Cosson
Keyword(s):  
Sperm Motility ◽  
Seminal Fluid ◽  
Mature Male ◽  
Sperm Maturation ◽  
Wolffian Duct ◽  
Testicular Spermatozoa ◽  
Acipenser Ruthenus ◽  
Sterlet Acipenser Ruthenus ◽  
Motility Parameters

The role of Ca2+ in sturgeon sperm maturation and motility was investigated. Sperm from mature male sterlets (Acipenser ruthenus) were collected from the Wolffian duct and testis 24 h after hormone induction. Testicular spermatozoa (TS) were incubated in Wolffian duct seminal fluid (WDSF) for 5 min at 20°C and were designated ‘TS after IVM’ (TSM). Sperm motility was activated in media with different ion compositions, with motility parameters analysed from standard video microscopy records. To investigate the role of calcium transport in the IVM process, IVM was performed (5 min at 20°C) in the presence of 2 mM EGTA, 100 µM Verapamil or 100 µM Tetracaine. No motility was observed in the case of TS (10 mM Tris, 25 mM NaCl, 50 mM Sucr with or without the addition of 2 mM EGTA). Both incubation of TS in WDSF and supplementation of the activation medium with Ca2+ led to sperm motility. The minimal Ca2+ concentration required for motility activation of Wolffian duct spermatozoa, TS and TSM was determined (1–2 nM for Wolffian duct spermatozoa and TSM; approximately 0.6 mM for TS). Motility was obtained after the addition of verapamil to the incubation medium during IVM, whereas the addition of EGTA completely suppressed motility, implying Ca2+ involvement in sturgeon sperm maturation. Further studies into the roles of Ca2+ transport in sturgeon sperm maturation and motility are required.

scholarly journals Correlation Between Ultrasonic Testicular Volume and Seminal Fluid Analysis in Men with Infertility

2021 ◽  
Vol 3 (1) ◽  
pp. 103-107
Author(s):  
Sadiq Abu ◽  
Hadijat O. Kolade-Yunusa ◽  
Terkaa Atim ◽  
F. Ehimatie Obakeye ◽  
Nuhu K. Dakum
Keyword(s):  
Sperm Motility ◽  
Teaching Hospital ◽  
Seminal Fluid ◽  
Sperm Concentration ◽  
Testicular Volume ◽  
Seminiferous Tubules ◽  
Testicular Function ◽  
Semen Volume ◽  
Significant Difference ◽  
The Mean

Background: Infertility is a major cause of marital disharmony in Nigeria because of the high premium placed on childbearing. Unfortunately, the blame is on the woman most times in Nigeria. Seminiferous tubules comprise 80-90% of testicular mass. Thus, the testicular volume is an index of spermatogenesis. Therefore, accurate testicular volume will help in assessing testicular function if there is no obstruction. This study was conducted to evaluate the correlation between testicular volume, measured by ultrasound and conventional sperm parameters (semen volume, sperm concentration, sperm motility and sperm morphology) in men with infertility presenting to Urology Division, University of Abuja Teaching Hospital, Gwagwalada Abuja. Methodology: This was a descriptive cross sectional study, male subjects with infertility presenting to University of Abuja Teaching Hospital were recruited into the study. The testicular volume of all the subjects was measured by ultrasonography. The semen samples were collected by the process of masturbation on day five of sexual abstinence and analyzed according to WHO criteria 2010. Collated data were analyzed using SPSS version 20.0. P-value<0.05 was taken as statistically significant. Results: The average total testicular volume (TTV) of men with infertility was 19.83 ml while mean testicular volume (MTV) was 10.24 ml. There was a statistically significant difference between MTV and semen volume (r=0.391, p=0.000), and sperm concentration (r=0.639, p=0.000). There was a weak and insignificant correlation between MTV and sperm motility and MTV and morphology (r=0.216, p=0.055 and r=0.076, p=0.502) respectively. Linear regression analysis showed significant impact of MTV (P<0.001) on sperm concentration with a critical MTV of ≤10ml.0 indicating poor testicular function (severe oligospermia) Conclusion: The mean ultrasonic testicular volume in men with infertility in this study was 10.24ml and there was a significant positive correlation of MTV with semen volume and sperm concentration. Although, there was correlation of MTV with sperm motility and morphology, this was not significant. The critical mean ultrasonic testicular volume indicating poor testicular function (severe oligospermia) was found to be 10ml. This study has shown that the mean ultrasonic testicular volume can be a pointer to semen volume and sperm concentration in evaluating men with infertility.

scholarly journals Sperm Na+ K+-ATPase and Ca2+-ATPase Activities: A Potential Predictive Parameter of Sperm Motility Disorder in Infertile Men

2018 ◽  
Vol 11 (1) ◽  
pp. 411-416
Author(s):  
Silvia W Lestari ◽  
Manggiasih D. Larasati ◽  
Indra G. Mansur ◽  
Ria Margiana
Keyword(s):  
Sperm Motility ◽  
Motility Disorder ◽  
Infertile Men

scholarly journals Evaluation of the Relationship between the Level of DHEA-S and Sex Hormones in Some Infertile Iraqi Men

2021 ◽  
Vol 8 (5) ◽  
pp. 130-136
Author(s):  
Khaled S. Abd ◽  
Adnan F. AL-Azzawie
Keyword(s):  
Sex Hormones ◽  
Seminal Fluid ◽  
Age Groups ◽  
Serum Levels ◽  
Dehydroepiandrosterone Sulfate ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Infertile Men ◽  
The Relationship

This study aims to evaluate serum levels of the Dehydroepiandrosterone sulfate (DHEA-S) and its relationship with some sex hormones such as Follicle stimulating hormone (FSH), Luteinizing hormone (LH), prolactin and testosterone hormone and the infertility type in some infertile Iraqi men. Blood and seminal fluid samples from (60) idiopathic male infertile and (60) healthful individuals as a control group aged (18 to 60 year) were collected from private clinics. Serum hormones (DHEA-S, FSH, LH, prolactin and testosterone) were measurement using Enzyme Linked Immunosorbent Assay (ELISA). The levels of DHEA-S, FSH, LH and prolactin are significantly higher (P≤0.01) expect of the testosterone level was significantly decreased (P≤ 0.01) in the infertile men as compared with control group. High significant differences (P≤0.01) were recorded when comparing the hormonal levels (DHEA-S, FSH, LH, prolactin and testosterone) according to the age groups. There are significant differences (P≤ 0.01) in levels of (DHEA-S, FSH, LH, prolactin and testosterone) in the infertility period.  Smoker infertile men have high levels (P≤ 0.05) in the DHEA-S and LH while have low levels in the prolactin hormones compared with control. Patients with family history have shown significant differences (P≤0.05) in the levels of DHEA-S, FSH, LH and prolactin. In conclusion, this study revealed significantly increase in the DHEA-S levels in the infertile men and negative correlation between DHEA-S and FSH. Therefore, DHEA-S has important role in the diagnosis and follow up of the male infertility.

scholarly journals The Presence of a Motility Inhibitor Within Spermatozoa May Explain the Poor Sperm Motility of Some Infertile Men

1986 ◽  
Vol 7 (4) ◽  
pp. 215-219 ◽  
Author(s):  
EVE de LAMIRANDE ◽  
RICHARD J. SHERINS ◽  
CLAUDE GAGNON
Keyword(s):  
Sperm Motility ◽  
The Poor ◽  
Infertile Men

Alpha-lipoic acid improves sperm motility in infertile men after varicocelectomy: a triple-blind randomized controlled trial

2020 ◽  
Vol 41 (6) ◽  
pp. 1084-1091
Author(s):  
Behzad Abbasi ◽  
Newsha Molavi ◽  
Marziyeh Tavalaee ◽  
Homayoun Abbasi ◽  
Mohammad H. Nasr-Esfahani
Keyword(s):  
Randomized Controlled Trial ◽  
Sperm Motility ◽  
Lipoic Acid ◽  
Controlled Trial ◽  
Alpha Lipoic Acid ◽  
Randomized Controlled ◽  
Infertile Men

Hormonal induction of Brycon cephalus (Characiformes, Characidae) to spermiation using D-ala6, pro9net-mGnRH + metoclopramide

Zygote ◽  
2015 ◽  
Vol 24 (3) ◽  
pp. 319-325 ◽  
Author(s):  
Cristiane Bashiyo-Silva ◽  
Raphael da Silva Costa ◽  
Douglas de Castro Ribeiro ◽  
José Augusto Senhorini ◽  
Rosicleire Veríssimo-Silveira ◽  
...  
Keyword(s):  
Single Dose ◽  
Sperm Motility ◽  
Significant Variation ◽  
Seminal Fluid ◽  
Physiological Solution ◽  
Control Group ◽  
Seminal Parameters ◽  
Live Fish ◽  
Hormonal Induction

SummaryThis study aimed to establish a hormonal induction protocol for spermiation of Brycon cephalus males, using Ala6, Pro9Net-mGnRH + metoclopramide (Ovopel®). Thus, 20 males were used divided into three inductor treatments [⅓ pellet/kg (T1), ⅔ pellet/kg (T2) and 1⅓ pellet/kg (T3)] and one control group (CO), which only received physiological solution applications (0.9% NaCl). All treatments were applied in a single dose. For evaluation of the availability of the treatment, the following seminal parameters were analyzed: seminal volume, subjective spermatic motility, duration of motility, pH, osmolality and spermatic concentration. T3 showed the highest seminal volume (4.66 ± 1.52 ml), and was significantly different in comparison with T1 (2.0 ± 0.9 ml), T2 (3.5 ± 1.3 ml) and CO (2.3 ± 1.2 ml). In relation to spermatic motility, T2 and T3 showed significantly higher levels [5, (81–100%)]. However, T3 showed significantly lower average sperm motility duration than T1, T2 and CO (30 ± 7 s; 28 ± 6 s; 32 ± 8 s, respectively). With regard to the seminal parameters of spermatic concentration, pH and osmolality, no significant variation was verified among treatments. In conclusion, mGnRH + metoclopramide used for hormonal induction of B. cephalus reproduction does not induce changes related to spermatic concentration, pH and osmolality parameters of the seminal fluid and the most adequate doses among tested treatments were ⅔ pellet/kg live fish.

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