scholarly journals Correlation Between Ultrasonic Testicular Volume and Seminal Fluid Analysis in Men with Infertility

2021 ◽  
Vol 3 (1) ◽  
pp. 103-107
Author(s):  
Sadiq Abu ◽  
Hadijat O. Kolade-Yunusa ◽  
Terkaa Atim ◽  
F. Ehimatie Obakeye ◽  
Nuhu K. Dakum
Keyword(s):  
Sperm Motility ◽  
Teaching Hospital ◽  
Seminal Fluid ◽  
Sperm Concentration ◽  
Testicular Volume ◽  
Seminiferous Tubules ◽  
Testicular Function ◽  
Semen Volume ◽  
Significant Difference ◽  
The Mean

Background: Infertility is a major cause of marital disharmony in Nigeria because of the high premium placed on childbearing. Unfortunately, the blame is on the woman most times in Nigeria. Seminiferous tubules comprise 80-90% of testicular mass. Thus, the testicular volume is an index of spermatogenesis. Therefore, accurate testicular volume will help in assessing testicular function if there is no obstruction. This study was conducted to evaluate the correlation between testicular volume, measured by ultrasound and conventional sperm parameters (semen volume, sperm concentration, sperm motility and sperm morphology) in men with infertility presenting to Urology Division, University of Abuja Teaching Hospital, Gwagwalada Abuja. Methodology: This was a descriptive cross sectional study, male subjects with infertility presenting to University of Abuja Teaching Hospital were recruited into the study. The testicular volume of all the subjects was measured by ultrasonography. The semen samples were collected by the process of masturbation on day five of sexual abstinence and analyzed according to WHO criteria 2010. Collated data were analyzed using SPSS version 20.0. P-value<0.05 was taken as statistically significant. Results: The average total testicular volume (TTV) of men with infertility was 19.83 ml while mean testicular volume (MTV) was 10.24 ml. There was a statistically significant difference between MTV and semen volume (r=0.391, p=0.000), and sperm concentration (r=0.639, p=0.000). There was a weak and insignificant correlation between MTV and sperm motility and MTV and morphology (r=0.216, p=0.055 and r=0.076, p=0.502) respectively. Linear regression analysis showed significant impact of MTV (P<0.001) on sperm concentration with a critical MTV of ≤10ml.0 indicating poor testicular function (severe oligospermia) Conclusion: The mean ultrasonic testicular volume in men with infertility in this study was 10.24ml and there was a significant positive correlation of MTV with semen volume and sperm concentration. Although, there was correlation of MTV with sperm motility and morphology, this was not significant. The critical mean ultrasonic testicular volume indicating poor testicular function (severe oligospermia) was found to be 10ml. This study has shown that the mean ultrasonic testicular volume can be a pointer to semen volume and sperm concentration in evaluating men with infertility.

Effect of different extenders on quality of frozen-thawed boar semen

2015 ◽  
Vol 49 (6) ◽  
Author(s):  
S Frydrychová ◽  
A Lustyková ◽  
E Václavková ◽  
J Lipenský ◽  
M Rozkot
Keyword(s):  
Sperm Motility ◽  
Aspartate Aminotransferase ◽  
Sperm Concentration ◽  
Holding Time ◽  
Holding Period ◽  
Semen Volume ◽  
Significant Difference ◽  
Boar Semen ◽  
Fresh Semen

The objective of this study was to investigate the effect of using different extenders <italic>viz.</italic> Androhep, Safecell Plus and SUS during cryopreservation on quality of frozen-thawed boar semen. Semen volume, sperm motility, sperm concentration, percentage of morphologically abnormal spermatozoa, total number of spermatozoa per ejaculate and activity of the enzyme aspartate aminotransferase (AST) were assessed in fresh semen collected from 39 fertile AI boars. Semen from each boar was divided into three portions and diluted 1:1.5 in extender Androhep, Safecell Plus and SUS and keep at 17°C for 15-h holding time before cryopreservation. Then sperm was cryopreserved. Straws were thawed in a water bath at 38°C for 40s and post-thaw sperm motility with AST activity was assessed. Significant difference in post-thaw sperm motility was found between extender Androhep and Safecell Plus (P<0.05). AST activity did not differ significantly between tested extenders (P>0.05). In conclusion, the results of the study indicate that using Safecell Plus extender during holding period before cryopreservation significantly affected post-thaw sperm motility.

scholarly journals Conception rate following intra-cervical artificial insemination using frozen semen at field level in indigenous sheep of Bangladesh

2018 ◽  
Vol 4 (1) ◽  
pp. 55-62 ◽  
Author(s):  
Md Abdullah Al Mansur ◽  
Md Golam Shahi Alam ◽  
Pankaj Kumar Jha ◽  
Md Asaduzzaman Rimon ◽  
Nazmun Naher ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Sperm Concentration ◽  
Conception Rate ◽  
Indigenous Sheep ◽  
Field Level ◽  
Frozen Semen ◽  
Significant Difference ◽  
Conception Rates ◽  
Viability Percentage ◽  
The Mean

This study was undertaken to study the AI conception rate using frozen semen at field level. Five farms in Mymensingh, Bangladesh were selected for AI Trial in field ewes. Four rams were selected for semen collection, evaluation, and frozen semen production and further to study conception rate followed by intra-cervical AI in both natural and synchronized ewes. Conception rate were confirmed by non-returned rate and ultrasound scanning at 30-40 days of post insemination. The volume, colour, mass activity, sperm motility, viability, concentration, HOST +ve (%) and normal spermatozoa percentages were 0.8±0.3 ml, 3.9±0.3, 4.4±0.6, 81.3±5.0%, 90.0±4.0%, 3519.0±545.6x106/ml, 87.4±3.3% and 85.6±1.8%, respectively. The sperm concentration of ram R#6 was significantly higher (P<0.05) (4120.5±93.5x106/ml) compared with other rams. The mean motility and viability of pre-dilution, 120 minutes of addition of Part-A, 240 minutes of addition of Part-B and post-thaw were (83.8±4.8%, 81.3±2.5%, 80.0±4.1% and 41.3±9.5%) and (93.3±1.0%, 90.0±1.4%, 88.8±1.0% and 58.3±8.7%), respectively. There were no significant difference (P˃0.05) between pre-dilution and post-dilution sperm motility and viability percentage however, post-thaw sperm motility and viability significantly (P<0.05) decreased compare with the motility and viability of pre-dilution and post-dilution values. Motility and viability percentages of frozen semen did not decrease significantly (P> 0.05) with the increase of preservation time. The mean motility and viability at 24 hrs, day 7, day 15 and day 30 were 41.3±9.5%, 41.5±8.5%, 41.8±9.9% and 40.5±10.2%; and 59.0±10.1%, 58.5±7.7%, 59.0±8.8% and 57.8±8.3%, respectively. The conception rates in natural and synchronized estrous were 26.7% and 25%, respectively. There was no significant difference in conception rates between the natural and synchronized oestrous in field level. However, the present non-return rate and conception rate indicate the suitability of produced frozen semen application in the field level.Asian J. Med. Biol. Res. March 2018, 4(1): 55-62

Semen production of the American kestrel

1977 ◽  
Vol 55 (8) ◽  
pp. 1351-1358 ◽  
Author(s):  
David M. Bird ◽  
Paul C. Laguë
Keyword(s):  
Sperm Motility ◽  
Sperm Count ◽  
Sperm Concentration ◽  
Barometric Pressure ◽  
Weather Factors ◽  
American Kestrel ◽  
American Kestrels ◽  
Semen Volume ◽  
The Mean ◽  
Egg Fertility

In 1975, semen was collected by massage from 18 randomly selected captive male American kestrels (Falco sparverius). The period of semen production extended for 103 days, beginning on March 19, while the mean duration of semen production was 73.6 days. Under natural light, semen production began at [Formula: see text] of daily light, peaked about [Formula: see text], and decline considerably around June 21 at [Formula: see text]. Weather factors, i.e., temperature, barometric pressure, did not significantly (P > 0.05) affect semen collections or quality.The following means were calculated: semen volume, 12 μl; sperm concentration, 31 000/mm3; sperm count per ejaculate, 416 000; motility score, 78%; contamination with epithelial debris and urates, 67%; and semen colour, very pale to pale amber.The following significant (P < 0.05) correlations were found: body weight with semen volume (0.48) and sperm count per ejaculate (0.54); egg fertility with sperm count per ejaculate (0.65); semen volume with sperm count per ejaculate (0.84) and sperm motility (0.54); sperm count per ejaculate with sperm concentration (0.78); and sperm motility with sperm concentration (0.59) and sperm count per ejaculate (0.75).

Impact of Male Aging on Semen Parameters

2021 ◽  
Vol 5 (2) ◽  
Author(s):  
Arzu Yurci ◽  
Nur Dokuzeylul Gungor
Keyword(s):  
Regression Analysis ◽  
Sperm Motility ◽  
Sperm Concentration ◽  
Paternal Age ◽  
World Health ◽  
Semen Parameters ◽  
Age Group ◽  
Male Age ◽  
Semen Volume ◽  
The Mean

Aim: To investigate the effect of increasing male age on the semen parameters including semen volume, sperm concentration, and progressive sperm motility. Materials and Methods: 1038 patients who applied to our IVF clinic for semen analysis or for infertility treatment were included in the study. The participants were divided into three age categories as follows: 680 participants (65.5%) aged 22-30, 139 participants (13.4%) aged 31-45 years, and 219 participants (21.1%) aged ≥45 years. Demographic and baseline clinical characteristics were recorded. The variables considered in this study were age and the following semen parameters: volume (ml); sperm concentration (millions/ml); and progressive sperm motility A+B (%). Normal semen values were defined based on the WHO criteria (World Health Organization, 2010). Results: The mean age of the participants was 33.1(± 4.8). The mean BMI of the participants was 24.1(± 2.8). The mean semen volume was 3.3 (± 1.5), the mean semen concentration was 43.6 (± 31.7), and the mean progressive sperm motility was 43.6 (± 10.1). There was a moderate negative correlation between paternal age and semen volume (r=-0.13) and sperm motility (r=-0.32), while there was a moderate positive correlation between paternal age and sperm concentration (r=0.24). The proportion of likelihood ratio measured between semen volume, sperm concentration, sperm motility and age was found to be 1 in patients between the ages of 22-30. The 22-30 age group does not have a decreasing or increasing effect on semen volume (OR: 1), sperm concentration (OR: 1) and motility (OR: 1). Regression analysis of patients in the 31-45 age group revealed no change in semen volume (OR: 0.99, 95% CI: 0.43-2.3, p <0.9), but an increase in sperm concentration (OR: 1.74, 95% CI: 1.13-2.7, p <0.01), and decreased sperm motility (OR: 0.28, 95% CI: 0.16-0.47, p <0.00). In the regression analysis of participants over the age of 45, semen volume (OR: 0.31, 95% CI: 0.19-0.53, p <0.00) and sperm motility (OR: 0.05, 95% CI: 0.03-0.08, p <0.00) decreased with age. No change was detected in the concentration (OR: 0.87, 95% CI: 0.63- 1.2, p <0.3). Conclusion: With increasing male age, semen volume and sperm motility begin to decrease. However, a significant decrease in sperm concentration does not occur until the age of 45.

scholarly journals Cryopreservation of Semen of Mithun and Siri Bulls

2014 ◽  
Vol 12 (2) ◽  
pp. 147-153 ◽  
Author(s):  
P Dorji ◽  
V Pattarajinda ◽  
T Vongprolub
Keyword(s):  
Plasma Membrane ◽  
Sperm Concentration ◽  
Membrane Integrity ◽  
Plasma Membrane Integrity ◽  
Soya Lecithin ◽  
Semen Volume ◽  
Significant Difference ◽  
Semen Characteristics ◽  
The Mean ◽  
Mass Activity

The objective of the study was to assess the semen characteristics of Jersey, Mithun and Siri breeds of bulls and to compare the suitability of Tris Eggyolk Citrate and commercial soya lecithin based BIOXcell extenders for cryopreservation of semen from the these breeds of bulls. The mean semen volume was 4.55± 1.57, 4.86±1.33 and 4.83±1.72 ml in Jersey, Mithun and Siri bulls, respectively. Mean mass activity of 3, mean initial motility of 78.0±4.22, 80±4.47 and 78.33±4.08%, mean sperm concentration of 1416±484.19, 1530.91±517.41, 1122.83±293.68 x106 and mean livability of 69.20±6.81, 70.45±8.42, 76.67±8.17% was found in Jersey, Mithun and Siri bulls, respectively. There is no significant difference in all above mentioned variables among the breeds. The post-thaw motility of semen cryopreserved in Tris Eggyolk Citrate extender was found to be 45.50 ± 4.38, 46.82 ± 6.81 and 47.50 ± 8.22% in Jersey, Mithun and Siri bulls, respectively. Whereas in BIOXcell extender, it was 45.50 ± 5.99, 46.36 ± 5.05 and 49.17 ± 5.85 % in Jersey, Mithun and Siri bulls, respectively. Similarly, the plasma membrane integrity of thawed spermatozoa in Tris eggyolk extender was 17.50±2.32, 21.00±3.12 and 21.58±3.83% in Jersey, Mithun and Siri bulls, respectively. Whereas in BIOXcell extender, it was found to be 20.45±2.86, 20.86±2.281 and 22.33±3.24% in Jersey, Mithun and Siri bulls, respectively. No significant difference (p>0.05) was observed in post-thaw motility as well as in plasma membrane integrity between extenders and between breeds, and there was no significant effect (p>0.05) of breed x extender interaction. It can be concluded that the semen characteristics of Siri and Mithun bulls fall with the range established for other breeds of bulls. Furthermore, both Tris eggyolk and BIOXcell extenders are equally suitable for the cryopreservation of semen from these three breeds of Bulls.DOI: http://dx.doi.org/10.3329/bjvm.v12i2.21277 Bangl. J. Vet. Med. (2014). 12 (2): 143-153 

scholarly journals The relationship between serum hormone levels (follicle-stimulating hormone, luteinizing hormone, total testosterone) and semen parameters

2015 ◽  
Vol 87 (3) ◽  
pp. 194 ◽  
Author(s):  
Mehmet Zeynel Keskin ◽  
Salih Budak ◽  
Tuǧba Zeyrek ◽  
Orçun Çelik ◽  
Oguz Mertoglu ◽  
...  
Keyword(s):  
Sperm Concentration ◽  
Reference Value ◽  
Semen Parameters ◽  
Total Testosterone ◽  
Testosterone Levels ◽  
Progressive Motility ◽  
Semen Volume ◽  
Significant Difference ◽  
Serum Hormone ◽  
Serum Gonadotropin

Objective: The aim of this study was to investigate the effect of serum gonadotropin and total testosterone levels on semen parameters. Materials and Methods: Three hundred and eighty-two patients that applied to a male infertility polyclinic were included in our study. Serum gonadotropin and total testosterone levels and semen parameters of the patients were analyzed during the first visit to the clinic. The reference FSH value was 1.5-12.4 mIU/mL, that of LH was 1.7-8.6 mIU/mL and the reference value for total testosterone was 249-836 ng/dL. Results: While there was no statistically significant difference between the patients with low gonadotropin levels and the controls regarding any of the semen parameters (p &gt; 0.05), there was a strong statistically significant difference between the patients with high gonadotropin levels and the controls regarding sperm concentration (p = 0.000), total motility (p = 0.000), progressive motility (p = 0.000), and morphology (p = 0.000). There was a strong statistically significant difference between the patients with low testosterone levels and the controls regarding total motility (p = 0.012) and progressive motility (p = 0.010), and a weak statistically significant difference in morphology (p = 0.042). There was no statistically significant difference in semen volume or sperm concentration (p &gt; 0.05). There was no statistically significant difference in any of the semen parameters between the patients with high testosterone levels and the controls (p &gt; 0.05). Conclusions: Our findings especially regarding LH and T levels are not in agreement with previous reports. In this regard, there is a need for larger-scale and randomized trials to resolve this discrepancy.

scholarly journals Evaluating the ability of semen cryopreservation of Blance Blue Belge bull cattle in Vietnam

2021 ◽  
Vol 19 (2) ◽  
pp. 237-244
Author(s):  
Nguyen Huu Duc ◽  
Pham Thu Giang ◽  
Tran Thi Binh Nguyen ◽  
Nguyen Thi Mai ◽  
Bui Dai Phong
Keyword(s):  
Sperm Motility ◽  
Sperm Concentration ◽  
Joint Stock Company ◽  
Stock Company ◽  
Cattle Breeding ◽  
Semen Cryopreservation ◽  
Semen Collection ◽  
Frozen Semen ◽  
Semen Volume ◽  
Fresh Semen

The objective of this study was to determine the semen cryopreservation capacity of BBB bulls in Hanoi-Vietnam. Research conducted on the fresh semen collected from 05 BBB bulls. Results showed that semen color was normal (milky white, ivory white, ivory yellow), semen volume ranged from 6.35 mL to 7.48 mL (P <0.05), initial motility of semen ranged from 80.53% to 82.92% (P <0.05), sperm concentration in semen  ranged from 1.02 x 109 sperms/ml to 1.12 x 109 sperms/mL (P <0.05), abnormal sperm ratio ranged from 6.45% to 8.12% (P <0.05), alive sperm ratio ranged from 76.34% to 82.97% (P <0.05), sperm motility after thawing from straw semen ranged from 71.33% to 75.92% (P<0.05). In conclusion, successfully semen collection from 05 breeding BBB bulls at Hanoi Cattle Breeding Joint Stock Company, semen samples had normal color and good quantity and quality, suitable for production of frozen semen; and semen cryopreservation of straws of the 05 bull BBB semen mentioned at -196oC, sperm motility after freezing-thawing reached the economic and technical norms of 675/2014 of the Ministry of Agriculture and Rural Development.

scholarly journals The Effect of Cyperus esculentus on Sperm Function Parameters in Prepubertal Mice as a Model for Human

2010 ◽  
Vol 7 (1) ◽  
pp. 389-393 ◽  
Author(s):  
Baghdad Science Journal
Keyword(s):  
Body Weight ◽  
Sperm Motility ◽  
Sperm Concentration ◽  
Sperm Function ◽  
Alcoholic Extract ◽  
Cyperus Esculentus ◽  
Alcohol Extract ◽  
The Mean ◽  
Group 2 ◽  
And Control

The objective of this work was to study the effect of oral administration of Cyperus esculentus (CE) and its alcoholic extract on sperm function parameters in prepubertal mice as a model for human .The animals were divided into three groups each contains 6 animals .Group 1 was treated with 150 mg/ kg body weight /day of crude CE, group 2 was treated with same dose of alcohol extract of CE and group 3 regarded as control throughout six weeks period. The results showed a significant (p> 0.05) increase in the mean of sperm concentration ,sperm motility percent and progressive sperm motility between treated groups and control . There was no differences among groups in the mean of sperm normal morphology and sperm viability . No significant differences was recorded in the mean of body weight among groups throughout the study. The results revealed that the administration of Cyperus esculentus may enhance certain sperm characters in prepubertal mice without affecting body weight.

scholarly journals Cross Sectional Study on Exposure to BPA and Phthalates and Semen Parameters in Men Attending a Fertility Center

2020 ◽  
Vol 17 (2) ◽  
pp. 489 ◽  
Author(s):  
Lidia Caporossi ◽  
Alessandra Alteri ◽  
Giovanni Campo ◽  
Enrico Paci ◽  
Giovanna Tranfo ◽  
...  
Keyword(s):  
High Performance ◽  
Seminal Fluid ◽  
Sperm Concentration ◽  
Chemical Exposure ◽  
Cross Sectional Study ◽  
Male Reproduction ◽  
Food Storage ◽  
Semen Parameters ◽  
Cross Sectional ◽  
Semen Volume

Among the possible risk factors for male reproduction, exposure to phthalates and alkylphenols is widely documented. This study evaluated the possible association between chemical exposure and the quality of the seminal fluid of 105 subjects in a fertility clinic. The urinary levels of seven phthalate metabolites (monoethylphthalate, MEP; monobenzylphthalate, MBzP; mono n-butylphthalate, MnBP; mono-(2-ethylhexyl) phthalate, MEHP; mono(2-ethyl-5-hydroxyhexyl) phthalate, MEHHP; mono-n-octylphthalate, MnOP; mono-isononylphthalate, MiNP) and bisphenol A (BPA), were analysed by high performance liquid chromatography/tandem mass spectrometry HPLC/MS/MS. The regression analysis showed that the semen volume was positively associated with MnBP, MnOP and BPA levels while was negatively associated with MiNP levels. The sperm concentration had a significant inverse relationship with MEP levels. A negative association was found between the use of plastic containers for food storage (p = 0.037) and semen volume (3.06 vs. 2.30 mL as average values, never vs daily). A significant positive correlation emerged (p < 0.005) between the consumption of canned food and the levels of BPA (2.81 vs. 0.14 µg/g creat as average values, daily vs. never) and between the use of perfumes and levels of MEP (389.86 vs. 48.68 µg/g creat, as average values, daily vs. never). No further statistically significant associations were found, even considering the working activity. Some evidence emerged about the possible link between exposure and seminal fluid quality: further case/control or prospective studies will allow us to confirm this causality hypothesis.

scholarly journals From Spermiogram to Bio-Functional Sperm Parameters: When and Why Request Them?

2020 ◽  
Vol 9 (2) ◽  
pp. 406 ◽  
Author(s):  
Rosita A. Condorelli ◽  
Aldo E. Calogero ◽  
Giorgio I. Russo ◽  
Sandro La Vignera
Keyword(s):  
Normal Form ◽  
Sperm Motility ◽  
Sperm Count ◽  
Semen Analysis ◽  
Sperm Concentration ◽  
Sperm Parameters ◽  
Sperm Parameter ◽  
Progressive Motility ◽  
Semen Volume ◽  
Chromatin Compactness

The aim of this experimental study was to evaluate whether infertile patients may benefit from the evaluation of bio-functional sperm parameters in addition to the conventional semen analysis. To accomplish this, we evaluated the correlation between conventional and bio-functional sperm parameters based on their percentile distribution in search of a potential threshold of these latter that associates with conventional sperm parameter abnormalities. The study was conducted on 577 unselected patients with infertility lasting at least 12 months. We identified cut-off values according to the median of the population for mitochondrial membrane potential (MMP), number of alive spermatozoa, and chromatin abnormality. High MMP (HMMP) (≥46.25%) was associated with sperm concentration, sperm count, progressive motility, and normal form. Low MMP (LMMP) (≥36.5%) was found to be associated with semen volume, sperm concentration, total sperm count, progressive motility, total motility, and normal form. The number of alive spermatozoa (≥71.7%) was associated with sperm concentration and progressive motility whereas abnormal chromatin compactness (≥21.10%) was associated with sperm concentration, total sperm count, and progressive motility. The data would suggest that, for every increase in the percentile category of sperm concentration, the risk of finding an HMMP≤46.25 is reduced by 0.4 and by 0.66 for a total sperm count. This risk is also reduced by 0.60 for every increase in the percentile category of sperm progressive motility and by 0.71 for total sperm motility. Each increment of percentile category of the following sperm parameter was followed by a decrease in the risk of finding an LMMP≤36.5: sperm concentration 1.66, total sperm count 1.28, sperm progressive motility 1.27, total sperm motility 1.76, and normal form 1.73. Lastly, the data showed that, for every increase in the percentile category of total sperm count, the risk of finding an abnormal chromatin compactness ≤21.10 is reduced by 1.25 (1.04–1.51, p < 0.05) and an increase of total sperm motility is associated with a reduced risk by 1.44 (1.12–1.85, p < 0.05). Results suggest a correlation between bio-functional and conventional sperm parameters that impact the sperm fertilizing potential. Therefore, the evaluation of bio-functional sperm parameters by flow cytometry may be useful to explain some cases of idiopathic male infertility.

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