Sperm motility of Prochilodus lineatus in relation to dilution rate and temperature of the activating medium

2010 ◽  
Vol 26 (5) ◽  
pp. 678-681 ◽  
Author(s):  
E. Romagosa ◽  
B. E. Souza ◽  
E. A. Sanches ◽  
D. M. Baggio ◽  
R. A. Bombardelli
Keyword(s):  
Dilution Rate ◽  
Sperm Motility ◽  
Prochilodus Lineatus

Effect of dilution rate on feline urethral sperm motility, viability, and DNA integrity

2014 ◽  
Vol 82 (9) ◽  
pp. 1273-1280 ◽  
Author(s):  
Sylwia Prochowska ◽  
Wojciech Niżański ◽  
Małgorzata Ochota ◽  
Agnieszka Partyka
Keyword(s):  
Dilution Rate ◽  
Sperm Motility ◽  
Dna Integrity

scholarly journals Effect of Sperm Concentration and Storage Temperature on Goat Spermatozoa during Liquid Storage

Biology ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 300 ◽  
Author(s):  
Sara Sadeghi ◽  
Raquel Del Gallego ◽  
Balma García-Colomer ◽  
Ernesto A. Gómez ◽  
Jesús L. Yániz ◽  
...  
Keyword(s):  
Dilution Rate ◽  
Dna Fragmentation ◽  
Sperm Motility ◽  
Storage Temperature ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Effect Of Temperature ◽  
Sperm Dna Fragmentation ◽  
Liquid Storage ◽  
Duration Of Storage

The use of cooled semen is relatively common in goats. There are a number of advantages of cooled semen doses, including easier handling of artificial insemination (AI) doses, transport, more AI doses per ejaculate, and higher fertility rates in comparison with frozen AI doses. However, cooled semen has a short shelf life. The objective of this study was to examine the effect of temperature and sperm concentration on the in vitro sperm quality during liquid storage for 48 h, including sperm motility and kinetics, response to oxidation, mitochondrial membrane potential (MMP) and DNA fragmentation in goats. Three experiments were performed. In the first, the effects of liquid preservation of semen at different temperatures (5 °C or 17 °C), durations (0, 24 and 48 h) and sperm concentrations (250 × 106 sperm/mL (1:2 dilution rate), 166.7 × 106 sperm/mL (1:3 dilution rate) or 50 × 106 sperm/mL (1:10 dilution rate)) on sperm motility and kinetics were studied. In the second experiment, the effect of temperature, sperm washing and concentration on sperm motility and DNA fragmentation was studied. Finally, the effect of sperm concentration and duration of storage at 5 °C on sperm motility, response to oxidative stress and MMP was examined. We found that refrigerated liquid storage of goat sperm impaired sperm quality, such as motility, MMP and response to oxidation, as storage time increased; however, sperm DNA fragmentation index was not significantly affected. Liquid storage at 5 °C preserved higher total motility than at 17 °C. Moreover, we observed that the reduction of sperm concentration below 500 × 106 sperm/mL did not seem to improve the quality of spermatozoa conserved in milk-based extender in the conditions tested.

scholarly journals Short-term cold storage of sperm from six neotropical characiformes fishes

2004 ◽  
Vol 47 (5) ◽  
pp. 799-804 ◽  
Author(s):  
Simone Marques ◽  
Hugo Pereira Godinho
Keyword(s):  
Sperm Motility ◽  
Cold Storage ◽  
Storage Duration ◽  
Plastic Tube ◽  
Prochilodus Lineatus ◽  
Practical Applications ◽  
Reproductive Management ◽  
Sperm Sample ◽  
Plastic Bag ◽  
Motility Rate

Sperm of the following Neotropical Characiformes fish species were tested for cold storage: Brycon lundii, Piaractus mesopotamicus, Leporinus elongatus, Leporinus friderici, Prochilodus lineatus and Prochilodus marggravii. Each sperm sample was split into two aliquots. The first was placed into a plastic bag with air or oxygen and the second, in a plastic tube with air. The samples were maintained at temperatures between 1.7-4.9 ºC. The rate of sperm motility was estimated using a 50 mM NaCl solution as the activating solution. The shortest sperm storage duration (7 h) was recorded for L. friderici, when the sperm motility rate reached ~ 30%, whereas the longest duration (20 h) was obtained with the sperm of P. lineatus. A fertilisation test using Prochilodus marggravii sperm refrigerated for 8 h yielded 88-90% of viable embryos. The refrigerated storage method could be of practical applications, especially in fish reproductive management at hatchery stations.

Effect of Caffeine Added to the Activating Solution on Sperm Motility of Fresh and Thawed Semen of Pacu,Piaractus mesopotamicus, and Curimba,Prochilodus lineatus

2014 ◽  
Vol 45 (1) ◽  
pp. 75-81 ◽  
Author(s):  
Aline F. S. Carvalho ◽  
Monica R. Ferreira Machado ◽  
Estefânia S. Andrade ◽  
Luis D. S. Murgas ◽  
Márcio G. Zangeronimo ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Prochilodus Lineatus ◽  
Piaractus Mesopotamicus ◽  
Activating Solution

scholarly journals Spermatozoa motility in the Persian sturgeon, Acipenser persicus: effects of pH, dilution rate, ions and osmolality

Reproduction ◽  
2004 ◽  
Vol 128 (6) ◽  
pp. 819-828 ◽  
Author(s):  
Sayyed Mohammad Hadi Alavi ◽  
Jacky Cosson ◽  
Mahmoud Karami ◽  
Bagher Mojazi Amiri ◽  
Mohammd Ali Akhoundzadeh
Keyword(s):  
Dilution Rate ◽  
Sperm Motility ◽  
Seminal Plasma ◽  
Seminal Fluid ◽  
Semen Quality ◽  
Total Duration ◽  
Basic Knowledge ◽  
Acipenser Persicus ◽  
High Osmolality

Sperm motility is a prerequisite factor determining semen quality and fertilizing capacity. The effects of environmental factors including pH, cations and osmolality as well as the role of dilution rate on sperm motility parameters in Acipenser persicus were studied. The best pH and dilution rate for activation of spermatozoa were pH 8.0 and dilution ratio 1:50. Ionic factors can stimulate the initiation of sperm activation. The maximum percentage of motile sperm and total duration of sperm motility were observed in solutions containing 25 mM NaCl, 0.2 mM KCl, 3 mM CaSO4, 10 mM MgSO4 and sucrose with an osmolality of 50 mosmol kg−1. The present study provides us with some basic knowledge about sturgeon spermatozoa biosensitivity to ionic and osmolality effects. A sensitivity of A. persicus sperm was observed after induction of activation of sperm motility in solution containing cations or sucrose with high osmolality. Concentrations more than 50 mM Na+, more than 1 mM K+, more than 3 mM Ca2+ and more than 10 mM Mg2+ had negative effects on sperm motility. Also, osmolality more than 100 mosmol kg−1 had an inhibitory effect. It is clear that ions and osmolality stimulate the motility of spermatozoa by changes in the properties of the plasma membrane including its potential and its ionic conductance. The inhibitory role of high osmolality of the swimming medium (more than 100 mosmol kg−1) and insufficient osmolality of the seminal plasma to inhibit semen motility suggested that osmolality is not the principal factor preventing sperm motility in seminal fluid but that K+ is a major inhibitory factor of sperm motility in seminal plasma.

scholarly journals Boar semen quality of the Přeštice black-pied breed (Short Communication)

2011 ◽  
Vol 54 (4) ◽  
pp. 374-380 ◽  
Author(s):  
S. Frydrychová ◽  
A. Lustyková ◽  
J. Lipenský ◽  
M. Rozkot
Keyword(s):  
Dilution Rate ◽  
Sperm Motility ◽  
Short Communication ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Quality Parameters ◽  
Test Period ◽  
Boar Semen ◽  
Natural Mating

Abstract. The objective of this study was to examine semen quality parameters for the Přeštice black-pied breed over a test period of 8 years while considering the potential effects of collection month and boar age. Ninety-nine ejaculates were collected using the gloved-hand technique from healthy and fertile mature boars from selected farms. Ejaculate volumes were relatively low because the boars were accustomed to natural mating. Sperm motility, sperm concentration, percentage of morphologically abnormal spermatozoa (MAS), and sperm motility after 24 h of storage in Androhep extender (dilution rate 1:1.5) were assessed. Significant differences were found in sperm concentration and MAS rate in relation to collection months and boar age in the monitored years (P<0.05). A tendency for MAS to increase with monitored years was observed. Significant differences in sperm motility and motility after 24 h of storage were only observed in relation to collection months (P<0.05). Results of this study detected effects due to collection month and boar age on boar semen quality during the monitored years.

220. Equine growth hormone enhances motility and extends longevity of ram spermatozoa in vitro

2005 ◽  
Vol 17 (9) ◽  
pp. 85 ◽  
Author(s):  
B. J. King ◽  
V. V. Da Silva ◽  
J. D. Harper ◽  
C. J. Scott ◽  
M. N. Sillence
Keyword(s):  
Growth Hormone ◽  
Dilution Rate ◽  
Sperm Motility ◽  
Room Temperature ◽  
Final Concentration ◽  
Bovine Growth Hormone ◽  
Semen Preservation ◽  
Sperm Survival ◽  
Motility Of Sperm

Sperm survival in vitro decreases with time at room temperature, but may be improved by treatment with recombinant bovine growth hormone in rats1, bulls2 and horses3. Two experiments investigated the effect of equine growth hormone (eGH) on the longevity of ram spermatozoa in vitro. The aim of experiment 1 was to determine if the addition eGH would improve the motility of ram spermatozoa after 24 h and identify any interaction with semen dilution rates used for ram semen preservation. Semen was collected from five mature Merino rams. Ejaculates were assessed for good quality and were diluted 1+50, 1+4, 1+3, 1+2 (semen+diluent) with a Tris-based cryoprotectant. Aliquots from each ram were mixed with eGH to achieve a final concentration of 100 ng/mL eGH and stored at 20°C for 24 h. Motility of spermatozoa was then assessed manually. eGH improved the motility of spermatozoa at all dilution rates compared to controls (P < 0.0001) but most markedly in the 1 + 3 and 1 + 2 samples (42.6 ± 0.8% eGH v. 22.7 ± 2.6% control; 40.5 ± 1.4% eGH v. 22.7 ± 2.2% control, respectively, P < 0.01). The aim of experiment 2 was to determine the optimum eGH concentration for improving sperm motility. eGH was added to aliquots of diluted semen (1+3 dilution rate) to produce samples with final concentrations of 1000, 100, 10 and 1 ng eGH/mL. The samples were placed in a water bath at 20°C for 24 h at which time the motility of sperm was assessed as before. Sperm motility was higher in the 100 ng/mL eGH sample (P < 0.05; 39.6 ± 0.7%) compared to other concentrations (1000 ng/mL 11.8 ± 0.7%, 10 ng/mL 21.5 ± 0.7% and 1 ng/mL 11.3±0.7%). We conclude that growth hormone is effective in promoting the longevity in vitro of ram spermatozoa stored at room temperature, and that this effect is concentration dependent. (1)Breier et al. (1996). Endocrinology 137, 4061–4064.(2)Sauerwein et al. (2000). Dom. Anim. Endocrinol. 18, 145–158.(3)Champion et al. (2002). Theriogenology 57, 1793–1800.

Extension of sperm motility leads to increased rates of fertilization and hatching in curimba, Prochilodus lineatus

2013 ◽  
Vol 30 (2) ◽  
pp. 329-333 ◽  
Author(s):  
E. E. Hernández Cuadrado ◽  
L. D. Solis Murgas ◽  
V. de Oliveira Felizardo ◽  
M. Rodrigues Ferreira ◽  
E. de Souza Andrade
Keyword(s):  
Sperm Motility ◽  
Prochilodus Lineatus

Arrangement of Mitochondria in Spermatozoa of the Mexican Axolotl, Ambystoma Mexicanum

1973 ◽  
Vol 31 ◽  
pp. 626-627
Author(s):  
Ezzatollah Keyhani ◽  
Larry F. Lemanski ◽  
Sharon L. Lemanski
Keyword(s):  
Plasma Membrane ◽  
Sperm Motility ◽  
Substrate Oxidation ◽  
Ambystoma Mexicanum ◽  
Axial Filament ◽  
Mexican Axolotl ◽  
Middle Piece

Energy for sperm motility is provided by both glycolytic and respiratory pathways. Mitochondria are involved in the latter pathway and conserve energy of substrate oxidation by coupling to phosphorylation. During spermatogenesis, the mitochondria undergo extensive transformation which in many species leads to the formation of a nebemkem. The nebemkem subsequently forms into a helix around the axial filament complex in the middle piece of spermatozoa.Immature spermatozoa of axolotls contain numerous small spherical mitochondria which are randomly distributed throughout the cytoplasm (Fig. 1). As maturation progresses, the mitochondria appear to migrate to the middle piece region where they become tightly packed to form a crystalline-like sheath. The cytoplasm in this region is no longer abundant (Fig. 2) and the plasma membrane is now closely apposed to the outside of the mitochondrial layer.

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