In vitro interactions between amphotericin B and other antifungal agents and rifampin against Fusarium spp.

ABSTRACT We performed in vitro antifungal checkerboard testing on 12 Aspergillus fumigatus clinical isolates (6 transplant recipients and 6 nontransplant patients) with three antifungal agents (amphotericin B, voriconazole, and caspofungin) and three immunosuppressants (FK506, cyclosporine, and rapamycin). We were not able to detect a difference in calcineurin inhibitor antifungal activity against isolates from transplant recipients and nontransplant patients.

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In Vitro Synergy Testing of Anidulafungin with Itraconazole, Voriconazole, and Amphotericin B against Aspergillus spp. and Fusarium spp

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.49.8.3572-3574.2005 ◽

2005 ◽

Vol 49 (8) ◽

pp. 3572-3574 ◽

Cited By ~ 62

Author(s):

Annie Philip ◽

Zekaver Odabasi ◽

Jose Rodriguez ◽

Victor L. Paetznick ◽

Enuo Chen ◽

...

Keyword(s):

Amphotericin B ◽

Drug Combinations ◽

Fusarium Spp ◽

Synergy Testing ◽

In Vitro Interactions

ABSTRACT The in vitro interactions of anidulafungin with itraconazole, voriconazole, and amphotericin B were evaluated by using the checkerboard method. For Aspergillus spp., anidulafungin with amphotericin B showed indifference for 16/26 isolates, while anidulafungin with either azole showed a synergy trend for 18/26 isolates. All drug combinations showed indifference for 7/7 Fusarium sp. isolates.

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In Vitro Interactions of Amphotericin B Combined with Non-antifungal Agents Against Rhodotorula mucilaginosa Strains

Mycopathologia ◽

10.1007/s11046-019-0317-6 ◽

2019 ◽

Vol 184 (1) ◽

pp. 35-43 ◽

Cited By ~ 1

Author(s):

Tatiana Borba Spader ◽

Mauricio Ramírez-Castrillón ◽

Patricia Valente ◽

Sydney Hartz Alves ◽

Luiz Carlos Severo

Keyword(s):

Amphotericin B ◽

Antifungal Agents ◽

Rhodotorula Mucilaginosa ◽

In Vitro Interactions

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In vitro interactions of antifungal agents against clinical isolates of Fusarium spp.

International Journal of Antimicrobial Agents ◽

10.1016/j.ijantimicag.2007.09.005 ◽

2008 ◽

Vol 31 (2) ◽

pp. 171-174 ◽

Cited By ~ 36

Author(s):

Susana Córdoba ◽

Laura Rodero ◽

Walter Vivot ◽

Rubén Abrantes ◽

Graciela Davel ◽

...

Keyword(s):

Antifungal Agents ◽

Clinical Isolates ◽

Fusarium Spp ◽

In Vitro Interactions

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In Vitro Interactions between Amphotericin B, Itraconazole, and Flucytosine against 21 Clinical Aspergillus Isolates Determined by Two Drug Interaction Models

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.48.6.2007-2013.2004 ◽

2004 ◽

Vol 48 (6) ◽

pp. 2007-2013 ◽

Cited By ~ 28

Author(s):

D. T. A. Te Dorsthorst ◽

P. E. Verweij ◽

J. F. G. M. Meis ◽

N. C. Punt ◽

J. W. Mouton

Keyword(s):

Drug Interaction ◽

Response Surface ◽

Amphotericin B ◽

Antifungal Agents ◽

Inhibitory Concentration ◽

Interaction Coefficient ◽

Surface Approach ◽

In Vitro Interaction ◽

In Vitro Interactions

ABSTRACT Combination therapy of flucytosine (5FC) with other antifungal agents could be of use for the treatment of invasive aspergillosis. However, interpretation of the results of in vitro interactions is problematic. The fractional inhibitory concentration (FIC) index is the most commonly used method, but it has several major drawbacks in characterizing antifungal drug interaction. Alternatively, a response surface approach using the concentration-effect relationship over the whole concentration range instead of just the MIC can be used. We determined the in vitro interactions between amphotericin B (AMB), itraconazole, and 5FC against 21 Aspergillus isolates with a broth microdilution checkerboard method that employs the dye MTT [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide]. FIC indices based on three different MIC endpoints (MIC-0, MIC-1, and MIC-2) and the interaction coefficient alpha were determined, the latter by estimation from the response surface approach described by Greco et al. (W. R. Greco, G. Bravo, and J. C. Parsons, Pharmacol. Rev. 47:331-385, 1995). The value obtained for the FIC index was found to be dependent on the MIC endpoint used and could be either synergistic, indifferent, or antagonistic. The response surface approach gave more consistent results. Of the three combinations tested, the AMB-5FC combination was the most potent in vitro against Aspergillus spp. We conclude that the use of the response surface approach for the interpretation of in vitro interaction studies of antifungals may be helpful in order to predict the nature and intensity of the drug interaction. However, the correlation of these results with clinical outcome remains difficult and needs to be further investigated.

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In Vitro Interactions of Approved and Novel Drugs against Paecilomyces spp

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.48.7.2727-2729.2004 ◽

2004 ◽

Vol 48 (7) ◽

pp. 2727-2729 ◽

Cited By ~ 33

Author(s):

Montserrat Ortoneda ◽

Javier Capilla ◽

F. Javier Pastor ◽

Isabel Pujol ◽

Clara Yustes ◽

...

Keyword(s):

Amphotericin B ◽

Antifungal Agents ◽

Antifungal Drugs ◽

Vitro Activity ◽

In Vitro Activity ◽

Paecilomyces Variotii ◽

Novel Drugs ◽

In Vitro Interactions

ABSTRACT We have evaluated the in vitro activity of 15 combinations of antifungal drugs (amphotericin B, itraconazole, voriconazole, albaconazole, ravuconazole, terbinafine, and micafungin) against four isolates of Paecilomyces variotii and three of P. lilacinus. The interaction of terbinafine with the four azoles was synergistic for 53% of the combinations, while the interactions of both amphotericin B and micafungin with the rest of antifungal agents were mainly indifferent.

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In Vitro Activity of Chlorhexidine Compared with Seven Antifungal Agents against 98 Fusarium Isolates Recovered from Fungal Keratitis Patients

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.02669-18 ◽

2019 ◽

Vol 63 (8) ◽

Cited By ~ 3

Author(s):

Claudy Oliveira dos Santos ◽

Eva Kolwijck ◽

Henrich A. van der Lee ◽

Marlou C. Tehupeiory-Kooreman ◽

Abdullah M. S. Al-Hatmi ◽

...

Keyword(s):

Amphotericin B ◽

Antifungal Agents ◽

Culture Collection ◽

Fungal Keratitis ◽

Molecular Techniques ◽

Temperate Climate ◽

Eye Drops ◽

Fungal Culture ◽

Content Type

ABSTRACT Fungal keratitis is a common but severe eye infection in tropical and subtropical areas of the world. In regions with a temperate climate, the frequency of infection is rising in patients with contact lenses and following trauma. Early and adequate therapy is important to prevent disease progression and loss of vision. The management of Fusarium keratitis is complex, and the optimal treatment is not well defined. We investigated the in vitro activity of chlorhexidine and seven antifungal agents against a well-characterized collection of Fusarium isolates recovered from patients with Fusarium keratitis. The fungus culture collection of the Center of Expertise in Mycology Radboudumc/CWZ was searched for Fusarium isolates that were cultured from cornea scrapings, ocular biopsy specimens, eye swabs, and contact lens fluid containers from patients with suspected keratitis. The Fusarium isolates that were cultured from patients with confirmed keratitis were all identified using conventional and molecular techniques. Antifungal susceptibility testing was performed according to the EUCAST broth microdilution reference method. The antifungal agents tested included amphotericin B, voriconazole, posaconazole, miconazole, natamycin, 5-fluorocytosine, and caspofungin. In addition, the activity of chlorhexidine was determined. The fungal culture collection contained 98 Fusarium isolates of confirmed fungal keratitis cases from 83 Dutch patients and 15 Tanzanian patients. The isolates were collected between 2007 and 2017. Fusarium oxysporum (n = 24, 24.5%) was the most frequently isolated species followed by Fusarium solani sensu stricto (n = 18, 18.4%) and Fusarium petroliphilum (n = 11, 11.2%). Amphotericin B showed the most favorable in vitro inhibition of Fusarium species followed by natamycin, voriconazole, and chlorhexidine, while 5-fluorocytosine, posaconazole, miconazole, and caspofungin showed no relevant inhibiting effect. However, chlorhexidine showed fungicidal activity against 90% of F. oxysporum strains and 100% of the F. solani strains. Our study supports the clinical efficacy of chlorhexidine and therefore warrants its further clinical evaluation for primary therapy of fungal keratitis, particularly in low and middle income countries where fungal keratitis is much more frequent and, currently, antifungal eye drops are often unavailable.

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In Vitro Activities of Isavuconazole and Other Antifungal Agents against Candida Bloodstream Isolates

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.01217-06 ◽

2007 ◽

Vol 51 (5) ◽

pp. 1818-1821 ◽

Cited By ~ 61

Author(s):

H. Seifert ◽

U. Aurbach ◽

D. Stefanik ◽

O. Cornely

Keyword(s):

Amphotericin B ◽

Antifungal Agents ◽

Tertiary Care ◽

Common Species ◽

University Hospital ◽

Phase Iii ◽

Highly Active ◽

Fluconazole Resistant ◽

Resistant Strains

ABSTRACT Isavuconazole is the active component of the new azole antifungal agent BAL8557, which is entering phase III clinical development. This study was conducted to compare the in vitro activities of isavuconazole and five other antifungal agents against 296 Candida isolates that were recovered consecutively from blood cultures between 1995 and 2004 at a tertiary care university hospital. Microdilution testing was done in accordance with CLSI (formerly NCCLS) guideline M27-A2 in RPMI-1640 MOPS (morpholinepropanesulfonic acid) broth. The antifungal agents tested were amphotericin B, flucytosine, fluconazole, itraconazole, voriconazole, and isavuconazole. C. albicans was the most common species, representing 57.1% of all isolates. There was no trend found in favor of non-Candida albicans species over time. In terms of MIC50s, isavuconazole was more active (0.004 mg/liter) than amphotericin B (0.5 mg/liter), itraconazole (0.008 mg/liter), voriconazole (0.03 mg/liter), flucytosine (0.125 mg/liter), and fluconazole (8 mg/liter). For isavuconazole, MIC50s/MIC90s ranged from 000.2/0.004 mg/liter for C. albicans to 0.25/0.5 mg/liter for C. glabrata. Two percent of isolates (C. glabrata and C. krusei) were resistant to fluconazole; C. albicans strains resistant to fluconazole were not detected. There were only two isolates with MICs for isavuconazole that were >0.5 mg/liter: both were C. glabrata isolates, and the MICs were 2 and 4 mg/liter, respectively. In conclusion, isavuconazole is highly active against Candida bloodstream isolates, including fluconazole-resistant strains. It was more active than itraconazole and voriconazole against C. albicans and C. glabrata and appears to be a promising agent against systemic Candida infections.

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Comparison of In Vitro Activity of Liposomal Nystatin againstAspergillus Species with Those of Nystatin, Amphotericin B (AB) Deoxycholate, AB Colloidal Dispersion, Liposomal AB, AB Lipid Complex, and Itraconazole

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.43.5.1264 ◽

1999 ◽

Vol 43 (5) ◽

pp. 1264-1266 ◽

Cited By ~ 37

Author(s):

Karen L. Oakley ◽

Caroline B. Moore ◽

David W. Denning

Keyword(s):

Amphotericin B ◽

Aspergillus Terreus ◽

Antifungal Agents ◽

Geometric Mean ◽

Colloidal Dispersion ◽

Vitro Activity ◽

In Vitro Activity ◽

Lipid Complex

ABSTRACT We compared the in vitro activity of liposomal nystatin (Nyotran) with those of other antifungal agents against 60Aspergillus isolates. Twelve isolates were itraconazole resistant. For all isolates, geometric mean (GM) MICs (micrograms per milliliter) were 2.30 for liposomal nystatin, 0.58 for itraconazole, 0.86 for amphotericin B (AB) deoxycholate, 9.51 for nystatin, 2.07 for liposomal AB, 2.57 for AB lipid complex, and 0.86 for AB colloidal dispersion. Aspergillus terreus (GM, 8.72 μg/ml; range, 8 to 16 μg/ml) was significantly less susceptible to all of the polyene drugs than all other species (P = 0.0001).

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