CHANGES IN RHODANESE CONTENT DURING PRIMARY SPREADING CULTURE OF EMBRYONIC KIDNEY CELLS REVEALED BY IMMUNO-ENZYMOLOGICAL METHOD

An aliquot from a continuous fermenter culture of baby hamster kidney cells (BHK-21 Clone PD-4) (Wistar) maintained in Ca free Eagle's Basal Medium containing 2% Kaolin adsorbed fetal calf serum was planted in spinner flasks at 300,000 cells per ml, total volume 600 ml. After equilibration for one day at 35°C to insure that cells were in log phase, the culture was infected with the M-33-AGMK25 BHK-219 strain of rubella at an input multiplicity of about 6 TCID50 per cell. The virus was identified with specific rubella antiserum.Preliminary experiments had shown that such cultures would reach a peak or plateau HA titer of approximately 1:64, 24 hrs after inoculation and would continue to yield virus for 6 to 12 days. One hundred ml aliquot harvests were withdrawn daily and the culture was returned to volume with growth medium and incubation continued. The harvested cells were spun down rapidly at 2500 rpm per 15 mins., fixed in 3.7% gluteraldehyde in Ca free phosphate buffer saline, and post fixed in osmium tetraoxide. After dehydration, the cells were embedded in Epon 812 and cured approximately 20 hrs at 60°C.

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1107: Evaluation of Cystine Transport in Cultured Human Kidney Cells: Primer to Gene Therapy of Cystinuria

The Journal of Urology ◽

10.1016/s0022-5347(18)35263-7 ◽

2005 ◽

Vol 173 (4S) ◽

pp. 300-300

Author(s):

Sreedhar Sagi ◽

Lutz Trojan ◽

Peter Aiken ◽

Maurice S. Michel ◽

Thomas Knoll

Keyword(s):

Gene Therapy ◽

Human Kidney ◽

Kidney Cells

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Effect of sodium butyrate on induction of cellular and viral DNA syntheses in polyoma virus-infected mouse kidney cells.

Journal of Virology ◽

10.1128/jvi.38.3.973-981.1981 ◽

1981 ◽

Vol 38 (3) ◽

pp. 973-981 ◽

Cited By ~ 22

Author(s):

E Wawra ◽

E Pöckl ◽

E Müllner ◽

E Wintersberger

Keyword(s):

Infected Mouse ◽

Sodium Butyrate ◽

Mouse Kidney ◽

Polyoma Virus ◽

Kidney Cells ◽

Viral Dna

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Distinct pathogenic effects of group B coxsackieviruses on human glomerular and tubular kidney cells.

Journal of Virology ◽

10.1128/jvi.71.12.9180-9187.1997 ◽

1997 ◽

Vol 71 (12) ◽

pp. 9180-9187 ◽

Cited By ~ 45

Author(s):

P G Conaldi ◽

L Biancone ◽

A Bottelli ◽

A De Martino ◽

G Camussi ◽

...

Keyword(s):

Kidney Cells ◽

Group B ◽

Pathogenic Effects

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Preparation, spectral properties and biological activities of 5-bromo-6-methyl-2'-deoxyuridine and 5-iodo-6-methyl-2'-deoxyuridine

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19802364 ◽

1980 ◽

Vol 45 (8) ◽

pp. 2364-2370 ◽

Cited By ~ 3

Author(s):

Antonín Holý ◽

Erik De Clercq

Keyword(s):

Antiviral Activity ◽

Spectral Properties ◽

De Novo ◽

Biological Activities ◽

Rabbit Kidney ◽

Kidney Cells ◽

Cd Spectra ◽

Methyl Derivatives ◽

Primary Rabbit

Reaction of 3',5'-di-O-benzoyl-6-methyl-2'-deoxyuridine (IIa) with elementary bromine or iodine afforded 5-halogeno derivatives IIc and IId which on methanolysis gave 5-bromo-6-methyl-2'-deoxyurine (Ic) and 5-iodo-6-methyl-2'-deoxyurine (Id), respectively. The CD spectra of Ic, Id and 6-methyl-2'-deoxyuridine (Ia) are compared and discussed with regard to determination of the nucleoside conformation. Unlike 5-bromo- and 5-iodo-2'-deoxyuridine, the 6-methyl derivatives Ic and Id exhibit neither antibacterial nor antiviral activity. Nor do they exert any antimetabolic effect on the de novo DNA synthesis in primary rabbit kidney cells.

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Characterization of Ascorbic Acid Uptake by Isolated Rat Kidney Cells

Journal of Nutrition ◽

10.1093/jn/121.1.57 ◽

1991 ◽

Vol 121 (1) ◽

pp. 57-64 ◽

Cited By ~ 20

Author(s):

Delores M. Bowers-Komro ◽

Donald B. McCormick

Keyword(s):

Ascorbic Acid ◽

Rat Kidney ◽

Acid Uptake ◽

Kidney Cells ◽

Isolated Rat Kidney ◽

Isolated Rat

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Studies of calcium-45 desaturation from kidney slices in flow-through chambers

AJP Regulatory Integrative and Comparative Physiology ◽

10.1152/ajpregu.1978.234.1.r34 ◽

1978 ◽

Vol 234 (1) ◽

pp. R34-R38

Author(s):

T. Uchikawa ◽

A. B. Borle

Keyword(s):

Kinetic Parameters ◽

Rat Kidney ◽

Kidney Cells ◽

Tissue Slices ◽

Kidney Slices ◽

System A ◽

Order Of Magnitude ◽

Exponential Equations ◽

Flow Through ◽

Best Fit

This paper describes a method to measure calcium fluxes and calcium exchangeable pools in tissue slices by continuous perifusion in flow-through chambers. 45Ca desaturation from rat kidney slices can be analyzed as in an open three-compartment catenary system. A set of equations is given to calculate all the relevant kinetic parameters from the triple exponential equations which best fit the desaturation curves. The results show that the kinetic parameters obtained in kidney slices by this new method are in the same order of magnitude as those previously observed in cultured monkey kidney cells.

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Antibody-Dependent Enhancement of Ebola Virus Infection

Journal of Virology ◽

10.1128/jvi.77.13.7539-7544.2003 ◽

2003 ◽

Vol 77 (13) ◽

pp. 7539-7544 ◽

Cited By ~ 119

Author(s):

Ayato Takada ◽

Heinz Feldmann ◽

Thomas G. Ksiazek ◽

Yoshihiro Kawaoka

Keyword(s):

Virus Infection ◽

Ebola Virus ◽

Human Populations ◽

Hemorrhagic Disease ◽

Viral Infectivity ◽

Kidney Cells ◽

Emerging Pathogens ◽

Viral Glycoprotein ◽

Antibody Dependent Enhancement ◽

Ebola Virus Infection

ABSTRACT Most strains of Ebola virus cause a rapidly fatal hemorrhagic disease in humans, yet there are still no biologic explanations that adequately account for the extreme virulence of these emerging pathogens. Here we show that Ebola Zaire virus infection in humans induces antibodies that enhance viral infectivity. Plasma or serum from convalescing patients enhanced the infection of primate kidney cells by the Zaire virus, and this enhancement was mediated by antibodies to the viral glycoprotein and by complement component C1q. Our results suggest a novel mechanism of antibody-dependent enhancement of Ebola virus infection, one that would account for the dire outcome of Ebola outbreaks in human populations.

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