scholarly journals Analysis In Vitro of Capacity of Fetal Fat Pad to Support Mammary Gland Embryogenesis. (fetus/mouse mammary gland/tissue culture/in vitro morphogenesis/biomatrix)

1987 ◽  
Vol 29 (4) ◽  
pp. 351-362 ◽  
Author(s):  
YUTAKA INAGUMA ◽  
YOSHIMI NISHI ◽  
TERUYO SAKAKURA ◽  
MORIAKI KUSAKABE ◽  
HOWARD L. HOSICK
Keyword(s):  
Tissue Culture ◽  
Mammary Gland ◽  
Mouse Mammary Gland ◽  
Fat Pad ◽  
In Vitro Morphogenesis ◽  
Culture In Vitro ◽  
Mammary Gland Tissue ◽  
Gland Tissue

A STUDY ON THE EFFECT OF INSULIN ON DNA, RNA AND PROTEIN SYNTHESIS IN MOUSE MAMMARY GLAND TISSUE IN ORGAN CULTURE

1971 ◽  
Vol 50 (2) ◽  
pp. 241-249 ◽  
Author(s):  
D. Y. WANG ◽  
VICKY AMOR
Keyword(s):  
Protein Synthesis ◽  
Mammary Gland ◽  
Organ Culture ◽  
Mouse Mammary Gland ◽  
Metabolic Inhibitors ◽  
Synthetic Activity ◽  
Insulin Stimulation ◽  
Mammary Gland Tissue ◽  
Gland Tissue ◽  
Rna And Protein Synthesis

SUMMARY The rates of synthesis of DNA, RNA and protein of mouse mammary gland explants in organ culture have been determined. Stimulation with insulin resulted in maximal rates of synthesis of these components, all occurring between 18 and 22 h of culture. The use of metabolic inhibitors of DNA, RNA or protein synthesis showed that after insulin stimulation, inhibition of any one of these processes was associated with a reduction in the synthesis of the other two components. Also the maximal rate of protein synthesis is governed by the net amount of RNA formed throughout the period of culture. Evidence is presented that the stimulation of DNA, RNA or protein synthesis by insulin is not due to increased transport of amino acids and that insulin appears to act rapidly on processes which subsequently lead to enhanced synthetic activity.

Glutathione–insulin transhydrogenase activity in pregnant mouse mammary gland: hormonal influences studied in tissue culture

1977 ◽  
Vol 55 (4) ◽  
pp. 340-345 ◽  
Author(s):  
B. M. Ferrier ◽  
J. M. Hendrie ◽  
C. A. Cardy
Keyword(s):  
Tissue Culture ◽  
Enzyme Activity ◽  
Mammary Gland ◽  
Culture Medium ◽  
Pregnant Mouse ◽  
Mouse Mammary Gland ◽  
Milk Secretion

Glutathione–insulin transhydrogenase (GIT) activity has been shown to be stimulated in culture of explants of pregnant mouse mammary gland by a mixture of insulin, cortisol, and prolactin. Since this hormone mixture stimulates lactogenesis in vitro it is possible that the increase in GIT activity is functionally related to one of the processes of milk secretion or ejection. Oxytocin is degraded by GIT and the interaction of this hormone with its mammary gland receptors may be influenced by the change in enzyme activity. The increase in GIT activity caused by insulin, cortisol, and prolactin in vitro can be prevented by the addition of progesterone or oxytocin to the culture medium.

BIOASSAY OF OXYTOCIN BY CHANGES IN LIGHT PENETRATION THROUGH RAT MAMMARY GLAND TISSUE IN VITRO

1971 ◽  
Vol 51 (4) ◽  
pp. 771-NP ◽  
Author(s):  
J. BÍLEK ◽  
I. MIKULÁŠ ◽  
J. SLABA ◽  
I. SKÁLA
Keyword(s):  
Mammary Gland ◽  
Wheatstone Bridge ◽  
Light Penetration ◽  
Mammary Gland Tissue ◽  
Gland Tissue ◽  
Rat Mammary Gland

SUMMARY A method for the bioassay of oxytocin in the lactating rat mammary gland in vitro is described. The changes in transparency induced by oxytocin in a flat segment of the gland are recorded by means of a CdS photoresistor and a simple Wheatstone bridge. The sensitivity of the assay in routine use is about 0·5–10 μu. oxytocin, the index of precision being about 0·1.

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