THE EFFECT OF NUTRITIONAL STATUS AND GROWTH HORMONE (GH) TREATMENT ON THE IN VITRO MITOGENIC RESPONSES OF MAMMARY GLAND TISSUE IN LAMBS

1989 ◽  
pp. 256
Author(s):  
S.D. Wheatley ◽  
J.M. Pell ◽  
I.A. Forsyth
Keyword(s):  
Growth Hormone ◽  
Mammary Gland ◽  
Nutritional Status ◽  
Gh Treatment ◽  
Mammary Gland Tissue ◽  
Gland Tissue

BIOASSAY OF OXYTOCIN BY CHANGES IN LIGHT PENETRATION THROUGH RAT MAMMARY GLAND TISSUE IN VITRO

1971 ◽  
Vol 51 (4) ◽  
pp. 771-NP ◽  
Author(s):  
J. BÍLEK ◽  
I. MIKULÁŠ ◽  
J. SLABA ◽  
I. SKÁLA
Keyword(s):  
Mammary Gland ◽  
Wheatstone Bridge ◽  
Light Penetration ◽  
Mammary Gland Tissue ◽  
Gland Tissue ◽  
Rat Mammary Gland

SUMMARY A method for the bioassay of oxytocin in the lactating rat mammary gland in vitro is described. The changes in transparency induced by oxytocin in a flat segment of the gland are recorded by means of a CdS photoresistor and a simple Wheatstone bridge. The sensitivity of the assay in routine use is about 0·5–10 μu. oxytocin, the index of precision being about 0·1.

scholarly journals Directed Expression of Tracheal Antimicrobial Peptide as a Treatment for Bovine-Associated Staphylococcus Aureus-Induced Mastitis in Mice

2021 ◽  
Vol 8 ◽  
Author(s):  
Zhipeng Zhang ◽  
Daijie Chen ◽  
Xubin Lu ◽  
Ruifeng Zhao ◽  
Zhi Chen ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Mammary Gland ◽  
Antimicrobial Peptide ◽  
Expression Vector ◽  
Bovine Mastitis ◽  
Specific Expression ◽  
Mammary Gland Tissue ◽  
Gland Tissue

Bovine mastitis is perplexing the dairy industry since the initiation of intensive dairy farming, which has caused a reduction in the productivity of cows and an escalation in costs. The use of antibiotics causes a series of problems, especially the formation of bacterial antimicrobial resistance. However, there are limited antibiotic-free therapeutic strategies that can effectively relieve bacterial infection of bovine mammary glands. Hence, in this study, we constructed a mammary gland tissue-specific expression vector carrying the antimicrobial peptide of bovine-derived tracheal antimicrobial peptide (TAP) and evaluated it in both primary bovine mammary epithelial cells (pBMECs) and mice. The results showed that the vector driven by the β-lactoglobulin gene (BLG) promoter could efficiently direct the expression of TAP in pBMECs and the mammary gland tissue of mice. In addition, significant antibacterial effects were observed in both in vitro and in vivo experiments when introducing this vector to bovine-associated Staphylococcus aureus-treated pBMECs and mice, respectively. This study demonstrated that the mammary gland tissue-specific expression vector could be used to introduce antimicrobial peptide both in in vitro and in vivo and will provide a new therapeutic strategy in the treatment of bovine mastitis.

A histological and biochemical study of hormone-dependent differentiation of mammary gland tissue in vitro

1966 ◽  
Vol 13 (2) ◽  
pp. 266-281 ◽  
Author(s):  
Frank E. Stockdale ◽  
William G. Juergens ◽  
Yale J. Topper
Keyword(s):  
Mammary Gland ◽  
Biochemical Study ◽  
Mammary Gland Tissue ◽  
Gland Tissue
Sign in / Sign up

Export Citation Format

Share Document