THE INFLUENCE OF AGE OF TEMPLATE DNA DERIVED FROM ARCHIVAL TISSUE ON THE OUTCOME OF THE POLYMERASE CHAIN REACTION

1994 ◽  
Vol 64 (8) ◽  
pp. 558-559 ◽  
Author(s):  
P. J. Hewett ◽  
F. Firgaira ◽  
A. Morley
Keyword(s):  
Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Archival Tissue ◽  
Polymerase Chain ◽  
Template Dna

scholarly journals A method for mapping germ line sequences in Tetrahymena thermophila using the polymerase chain reaction.

Genetics ◽  
1994 ◽  
Vol 137 (1) ◽  
pp. 95-106 ◽  
Author(s):  
D Cassidy-Hanley ◽  
M C Yao ◽  
P J Bruns
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Sequences ◽  
Tetrahymena Thermophila ◽  
Germ Line ◽  
Repetitive Sequences ◽  
Mapping Technique ◽  
Ciliated Protozoan ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Template Dna

Abstract A method for mapping DNA sequences to specific germinal chromosomes in the ciliated protozoan Tetrahymena thermophila has been developed. This mapping technique (PCR mapping) utilizes the polymerase chain reaction and template DNA derived from nullisomic strains to directly assign micronuclear DNA sequences to specific micronuclear chromosomes. Using this technique, a number of unique sequences and short repetitive sequences flanked by unique sequences have been mapped to four of the five germinal chromosomes.

Preparation of Polymerase Chain Reaction Template DNA from Mouse Tail Tissue

2019 ◽  
Vol 2019 (4) ◽  
pp. pdb.prot092700
Author(s):  
Richard Behringer ◽  
Marina Gertsenstein ◽  
Kristina Vintersten Nagy ◽  
Andras Nagy
Keyword(s):  
Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Polymerase Chain Reaction Template ◽  
Polymerase Chain ◽  
Template Dna

scholarly journals MDR-1 GENE 1199 VARIANT PRIMER DESIGN TECHNIQUES IN PEDIATRIC PATIENT BUFFY COAT SAMPLES WITH LLA

2018 ◽  
Vol 5 (1) ◽  
pp. 105
Author(s):  
Putu Desy Yustinadewi ◽  
Putu Sanna Yustiantara ◽  
Inna Narayani
Keyword(s):  
Polymerase Chain Reaction ◽  
Single Nucleotide Polymorphism ◽  
Pediatric Patient ◽  
Buffy Coat ◽  
Nucleotide Polymorphism ◽  
Chain Reaction ◽  
Single Nucleotide ◽  
Polymerase Chain ◽  
Template Dna ◽  
Mdr 1

Single Nucleotide Polymorphism (SNP) 1199 dapat diidentifikasi menggunakan sampel buffy coat dengan metode Polymerase Chain Reaction (PCR). Komponen- komponen yang diperlukan pada proses PCR adalah template DNA; sepasang primer, yaitu suatu oligonukleotida pendek yang mempunyai urutan nukleotida yang komplementer dengan urutan nukleotida DNA templat; dNTPs (Deoxynucleotide triphosphates); buffer PCR; magnesium klorida (MgCl2) dan enzim polimerase DNA (Handoyo dan Rudiretna, 2001). Primer sangat mempengaruhi spesifitas dan sensitivitas reaksi PCR. Rancangan suatu primer merupakan salah satu parameter penentu keberhasilan suatu proses PCR (Ebd-Elsalam, 2003). Primer untuk sekuensing gen MDR-1 variant 1199 berhasil didesain dalam kondisi terbaik. Panjang sekuen primer forward sejumlah 21 oligonukleotida dan reverse sejumlah 20 oligonukleotida dengan fragmen sebesar 225 pb.

scholarly journals Evaluation of quantitative polymerase chain reaction to assessnosZgene prevalence in mixed microbial communities

2007 ◽  
Vol 53 (5) ◽  
pp. 636-642 ◽  
Author(s):  
Steven D. Siciliano ◽  
Wai Ma ◽  
Shane Powell
Keyword(s):  
Polymerase Chain Reaction ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Ralstonia Eutropha ◽  
Quantitative Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Pcr Products ◽  
Gradient Gel Electrophoresis ◽  
Total Community ◽  
Polymerase Chain ◽  
Template Dna

The usefulness of quantitative polymerase chain reaction (QPCR) to measure nosZ gene prevalence in a multi-template reaction was assessed by comparing 19 nosZ template DNA samples and 91 model communities. Efficiencies of the QPCR varied but were not significantly different among nosZ genotypes and were not linked to genetic distance from Ralstonia eutropha . nosZ genotype QPCR efficiencies obtained from isolated denitrifiers were higher (84.8%) than those obtained from excised denaturing gradient gel electrophoresis bands or clones of PCR products from total community DNA (ca. 60%). Analysis of the model communities indicated that QPCR accurately predicts gene prevalence in communities composed of up to six templates.

Detection of Pork in High Tenperature Processed Food by Taqman Real-Time Polymerase Chain Reaction

2012 ◽  
Vol 550-553 ◽  
pp. 1919-1923
Author(s):  
Li Li Fan ◽  
Hong Liu Ding ◽  
Chun Ling Fu ◽  
Pei Li
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Processed Food ◽  
Chain Reaction ◽  
Highly Sensitive ◽  
Mitochondrial Cytochrome B ◽  
Polymerase Chain ◽  
Processed Products ◽  
Template Dna

A rapid real-time polymerase chain reaction (PCR) based on Taqman technique has been developed for the qualitative analysis of pork in high temperature processed food. Specific primers and probe were designed to the conserved region of the mitochondrial cytochrome b gene, amplifying a 98bp fragment. Speciation was achieved using this assay, showing no cross-amplification with cattle, sheep, chicken and duck DNA while Ct (cycle threshold) of PCR for negative sample was limited to 35 cycles. This assay was sensitive to detect 1pg of pork template DNA. Meat mixtures spiked with 1-10% pork were successfully tested, which demonstrated the suitability of the assay for determination of swine-derived ingredient in food. The system is highly sensitive and specific, and will be useful for swine species identification of animal-derived processed products.

A polymerase chain reaction method for detecting dwarf mistletoe infection in Douglas-fir and western larch

1999 ◽  
Vol 29 (9) ◽  
pp. 1317-1321 ◽  
Author(s):  
M Marler ◽  
D Pedersen ◽  
T Mitchell-Olds ◽  
R M Callaway
Keyword(s):  
Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Method ◽  
Positive Control ◽  
Rbcl Gene ◽  
Larix Occidentalis ◽  
Chain Reaction ◽  
Dwarf Mistletoe ◽  
Pcr Product ◽  
Polymerase Chain ◽  
Template Dna

Early detection and management of dwarf mistletoe (Arceuthobium spp.) is currently limited by the inability to rapidly detect infection during the 2- to 5-year endophyte phase of the parasite. We describe a polymerase chain reaction (PCR) technique for detecting Arceuthobium douglasii Engelm. and Arceuthobium laricis Engelm. in tissues of its hosts, Pseudotsuga menziesii (Mirb.) Franco and Larix occidentalis Nutt. DNA was extracted from branches of 15 infected and 15 uninfected P. menziesii. The PCR product amplified by using the Arceuthobium specific primer in the rbcL gene from Arceuthobium template DNA was a fragment of 708 pairs of bases in length. This product was amplified from all branches that were visibly infected, but the fragment was not generated from any samples known to be uninfected. The PCR product from conifer DNA was a fragment of 385 pairs of bases in length and was not amplified from pure mistletoe DNA; this was amplified as an internal positive control. The primers developed for P. menziesii and A. douglasii also worked on L. occidentalis and A. laricis. This method detected mistletoe DNA in 7 of 29 P. menziesii branches and 3 of 21 L. occidentalis branches that did not have external symptoms of infection and are presumed to be the result of the endophyte phase. This method provides a useful tool for experimental applications and for managing the spread of dwarf mistletoe.

scholarly journals DIRECT AND SENSITIVE DETECTION OF TRYPANOSOMA EVANSI BY POLYMERASE CHAIN REACTION

1999 ◽  
Vol 47 (3) ◽  
pp. 351-359 ◽  
Author(s):  
S. Omanwar ◽  
J. R. Rao ◽  
G. Butchaiah ◽  
S. H. Basagoudanavar ◽  
R. K. Singh
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Sequences ◽  
Animal Health ◽  
Trypanosoma Evansi ◽  
Chain Reaction ◽  
Wasting Disease ◽  
Specificity And Sensitivity ◽  
Sensitivity Level ◽  
Polymerase Chain ◽  
Template Dna

The mechanically transmitted haemoflagellate,Trypanosoma evansicauses 'surra', a wasting disease of domestic animals and is highly endemic in distribution in Southeast Asia. The detection ofT. evansiis important for improving the epizootiological and animal health status of the region. The specificity and sensitivity of polymerase chain reaction (PCR) using oligonucleotide primers constructed fromT. evansirepetitive DNA sequences were studied in the present investigation. Using the assay, it was possible to amplify template DNA ofT. evansiderived from buffaloes, camels and horses to a threshold sensitivity level of 0.5 pg and to detect DNA from as few as five organisms in 10 (l crude blood samples. Following experimental infection of calves with 5 × 105T. evansi, positive signals could be observed as early as 12 h post-infection. DNAs from two common haemoflagellates of cattle,Babesia bigeminaandTheileria annulatawere not amplified with the primers.

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