Heavy Metal Concentration (Pb, Cu, Cd, Zn) In Water And Sediments In Serangan Waters, Bali

Heavy metal pollution can disturb aquatic ecosystems and indicator of water quality. This study aims to determine the concentration of heavy metals (Pb, Cu, Cd, Zn) in water and sediments. This study was conducted in the waters of Serangan, South Denpasar District, Bali in June-August 2019. The research with each location taking 8 points of water and sediment sampling and measuring the quality of its waters. Water samples taken at the surface of the water as much as 330 ml and sediment samples taken as much as 500 grams. The method used is wet ashing preparation and analyzed by means of AAS (Atomic Absorbsion Spektrophotometri). The results of the average analysis of heavy metal concentrations in water are Pb 0 mg / L, Cd 0.438 mg / L, Cu 0 mg./L, and Zn 0.284 mg / L. The results of the analysis are compared with the standard quality value of sea water that the heavy metals Cd and Zn have exceeded the threshold. While the average concentration of heavy metals in sediments are Pb 17.444 mg / kg, Cd 18.093 mg / kg, Cu 113.981 mg / kg, and Zn 53.505 mg / kg. The concentration of Cd and Cu heavy metals exceeds the limits set by the ANZECC / ARMCANZ quality guidelines and the Canadian Council of Ministers of the Environment.

Analisis Filogenetik Cyprinus carpio Ruang Lingkup Asia Timur Dan Eropa Berdasarkan Genom Mitokondria

Carp (Cyprinus carpio L.) is one of the oldest and most commercially cultivated freshwater fish in the World. However, there are still many undetermined phylogenetic relationships and the origins of common goldfish lineages, which are an obstacle to the conservation and genetic reproduction of this species. In the process of phylogenetic analysis, researchers used mitochondrial genomes where the genomic DNA was obtained from homozygous double haploid clonal lines from domesticated Songpu strains, and the total genomic DNA was extracted and sequenced using next-generation sequencing technology. Complete mitochondrial genome sequencing of 11 individuals representing East Asia and Europe and phylogenetic analysis was performed. The purpose of this study is to provide information about the phenology of Cyprinus carpio fish, which is expected to be the basis for increasing understanding to determine the kinship relationship between Cyprinus carpio in East Asia and Europe. The results showed that the relationship between Cyprinus carpio color, Cyprinus carpio songpu mirror carp, Cyprinus carpio carpio, and Cyprinus carpio wuyuanensis was very close, this can be seen from the bootstrap value of 100%. Meanwhile, with Cyprinus carpio haematopterus has a bootstrap value of 70%. Meanwhile, with Cyprinus xingguonensis it has a bootstrap value of 66%. Furthermore, it can also be said that the Songpu mirror goldfish is closely related to C. carpio carpio which is also a member of the genus Cyprinu. The genus Cyprinu has a close relationship with the genus Catla. In the phylogenetic tree, this species of fish forms a very coherent group, supported by a high bootstrap value of 100% and an average genetic distance of 0.02. Key words: Mitochondrial genome, Phylogenetics, general Cyprinus carpio

Profil Metabolit Daun Kesambi (Schleichera oleosa) Berdasarkan Analisis Histokimia dan In Silico

Kesambi (Schleichera oleosa) is a plant belonging to the Sapindaceae familia. This study aims to determine the secondary metabolite compounds found in kesambi leaves through histochemical analysis and derivatives of active compounds in silico. Descriptive experimental research method was conducted in January using samples of kesambi plants that grow on the island of Madura. Histochemical analysis was carried out by preparing fresh leaves through lower leaf incisions with secondary metabolite detection reagents (CuSO4, FeCl3, Wagner, Sudan III, AlCl3 & FeCl3+NaCO3) then microscopic color changes were observed. In silico testing aims to determine the interaction of active compounds with ER? as a target for ER + breast cancer therapy through molecular docking. Supporting software used is KNApSAcK, Pubchem, Pass Online, PDB ID, PyRx, PyMol and Chimera 1.14. The results showed that through histochemical analysis six secondary metabolite compounds were identified, namely terpenoids, flavonoids, alkaloids, tannins, lipophils and phenols. While in silico analysis, the active compound is scopoletin which is derived from phenol, beta-sitosterol, betulin, betulinic acid, lupeol, lupeol acetate, schleicheol 1&2, schleicherastatin 1-7 which are derivatives of terpenoids. Based on the results of molecular docking, there are interactions of active compounds with 3ERT protein, the compounds that provide the most effective results as candidates for breast cancer drugs are lupeol acetate with a value of Root Mean Square Deviation (RMSD) lb 1,588 Å and ub 2,219 Å . Lupeol acetate compound is predicted to have activity as an Er? inhibitor against ER+ breast cancer. Keywords: Kesambi (Schleichera oleosa), histochemistry, molecular docking and ER?.

Deteksi Level Antibodi Pada Serum Darah Anjing Kintamani Pasca Vaksinasi Rabies dengan Direct ELISA

The success of rabies vaccination is characterized by the growth of seropositive antibody titers (?0.5 IU) after vaccination. One of the tests conducted to monitor antibody growth is ELISA (Enzyme Linked Immunosorbent Assay). This study aimed to determine the effect of different vaccine doses on the growth of rabies antibodies in kintamani dogs. The study was conducted using an experimental method with a study design using 2 variables, namely the difference in vaccine doses (0.5 and 0.75 cc) and the times of blood collection (1, 2 and 3 months after vaccination). Each vaccine dose was given to three kintamani dogs. Antibodies were measured using ELISA and data were analyzed with paired sample t-test between the treatment dose of 0.75 cc and 0.5 cc at months 1.2 and 3 after vaccination. The results showed that at first month the antibody titer were 2,12 IU at a dose of 0.75 cc and 3.72 IU at a dose of 0.5 cc. At second months, antibody titers were 7.74 IU (0.75 cc dose) and 10.85 IU (0.5 cc dose), while at third month, antibody titers were 5.73 IU (0.75 cc) and 9.00 IU (0.5 cc). Vaccine doses administered produce antibody titer levels that did not differ significantly between 0.75 cc and 0.5 cc doses. Keyword: Antibody, ELISA, Kintamani Dog, Rabies

Efek Ekstrak Daun Seledri (Apium graveolens L.) Terhadap Struktur Histologi Ginjal Tikus (Rattus norvegi

Ginjal adalah organ ekskresi penting untuk membuang sisa metabolisme tubuh. Batu ginjal dapat mempengaruhi fungsi ginjal. Seledri mengandung senyawa flavonoid yang dapat memperbaiki struktur histologis ginjal. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak seledri terhadap struktur histologis ginjal yaitu diameter glomerulus dan tubulus dengan endapan protein. Penelitian ini merupakan penelitian eksperimental dengan Rancangan Acak Lengkap (RAL). Kelompok perlakuan dibagi menjadi 4 kelompok yaitu, kontrol negatif (K-), tikus hanya diberi makan dan minum; kontrol positif (K +), tikus diinduksi Etilen glikol (EG) 0.75% + Ammonium klorida (AK) 2%, dosis 1 (D1), tikus diinduksi EG 0.75% + AK 2% dan ekstrak seledri 100 mg / kg, dosis 2 ( D2), tikus diinduksi EG 0,75% + AK 2% dan ekstrak seledri 200 mg / kgBB. Pemberian ekstrak seledri selama 14 hari secara gavage. Ekstrak seledri dengan dosis 100 dan 200 mg / kgBB mampu memperbaiki struktur histologis ginjal tikus yang diinduksi oleh EG 0,75% dan AK 2%. Kata kunci: seledri, diameter glomerulus, endapan protein

Kupu-Kupu Sebagai Daya Tarik Ekowisata Di Kawasan Pariwisata Lebih, Gianyar, Bali

Penelitian tentang kupu-kupu sebagai daya tarik ekowisata di kawasan pariwisata Lebih, Gianyar dilaksanakan pada tahun 2019. Tujuan penelitian ini adalah untuk mengetahui jenis-jenis kupu-kupu yang ditemukan di kawasan tersebut serta mengetahui potensinya sebagai daya tarik ekowisata. Sampel kupu-kupu diambil melalui eksplorasi lokasi-lokasi penelitian, serta data lain diambil melalui wawancara. Kupu-kupu yang ditemukan di lokasi ini diidentifikasi kemudian dicatat. Potensi kupu-kupu sebagai daya tarik ekowisata dianalisis berdasarkan persepsi wisatawan. Lokasi dimana kupu-kupu telah dipakai sebagai daya tarik ekowisata, serta bagaimana membuat paket ekowisata berbasis kupu-kupu (jika ada) dilaporkan. Hasil studi ini menunjukkan bahwa ada 30 species kupu-kupu teridentifikasi di Kawasan Pariwisata Lebih. Berdasarkan hasil observasi serta wawancara dapat disimpulkan, bahwa ekowisata berbasis kupu-kupu atau produk ekowisata berbasis kupu-kupu sudah ada satu buah di Kawasan Pariwisata Lebih. Kawasan Pariwisata Lebih memiliki potensi untuk mengembangkan ekowisata berbasis kupu-kupu. Potensi ini dapat dilihat dari adanya kupu-kupu beragam, 30 jenis sebagai atraksi ekowisata. Potensi ini dapat ditingkatkan dengan keberadaan atraksi lainnya termasuk diantaranya keberadaan atraksi budaya dan atraksi buatan. Dari segi aksesibilitas: Kawasan Pariwisata Lebih termasuk relatif mudah diakses, khususnya oleh fasilitas transport yang dikelola berbasis online. Keberadaan fasilitas pariwisata di kawasan Lebih telah meningkat. Dukungan untuk pengembangan Kawasan Pariwisata Lebih telah diberikan oleh pemerintah. Setelah menetapkan sembilan desa serta beberapa desa di sekitarnya sebagai kawasan pariwisata, pemerintah serta pihak-pihak lainnya telah mengembangkan fasilitas dan menyelenggarakan even-even pariwisata pada kawasan ini. Kata Kunci— Bali, ekowisata, Kawasan Pariwisata Lebih, kupu-kupu, potensi ekowisata

Kandungan Vitamin C dan Uji Organoleptik Lulur Rosella (Hibiscus sabdariffa L.)

Rosella tea is a ingredient that can be used for making scrubs because of its antioxidant content, but yet limited in cosmetic science. This research was aimed to find out the amount value of vitamin C and antioxidant activity in scrub that contains Rosella tea (Hibiscus sabdariffa L.) with variation of rice flour concentrations. This research was conducted at Analitic Laboratory, Faculty of Agricultural Technology and Soil Mechanical Laboratory, Faculty of Engineering, Udayana University from May until August 2018. The experiment was conducted using completely randomized design with seven treatments. Parameters observed were the amount values of vitamin C, antioxidant activities and organoleptic consists texture, colour, aroma and impression of Rosella scrub. The results of this results is the highest vitamin C and antioxidant content were found in rosella scrub with the percentage of 10% white rice flour and 80% red rice flour. The difference in body scrub treatment has a significant effect on proband judgments seen from the texture, color and aroma, while the difference in lulur treatment has no significant effect on proband judgments seen from the impression. Keyword: scrub, Rosella, vitamin C, antioxidant, organoleptic